Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2014.969249 http://hdl.handle.net/11449/171717 |
Resumo: | Well-established biofilms formed by Streptococcus mutans via exopolysaccharide matrix synthesis are firmly attached to tooth surfaces. Enhanced understanding of the physical properties of mature biofilms may lead to improved approaches to detaching or disassembling these highly organized and adhesive structures. Here, the mechanical stability of S. mutans biofilms was investigated by determining their ability to withstand measured applications of shear stress using a custom-built device. The data show that the initial biofilm bulk (~ 50% biomass) was removed after exposure to 0.184 and 0.449 N m−2 for 67 and 115 h old biofilms. However, removal of the remaining biofilm close to the surface was significantly reduced (vs initial bulk removal) even when shear forces were increased 10-fold. Treatment of biofilms with exopolysaccharide-digesting dextranase substantially compromised their mechanical stability and rigidity, resulting in bulk removal at a shear stress as low as 0.027 N m−2 and > a two-fold reduction in the storage modulus (G′). The data reveal how incremental increases in shear stress cause distinctive patterns of biofilm detachment, while demonstrating that the exopolysaccharide matrix modulates the resistance of biofilms to mechanical clearance. |
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Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forcesdextranaseexopolysaccharide matrixmechanical stabilityrheometryS. mutans biofilmshear stressWell-established biofilms formed by Streptococcus mutans via exopolysaccharide matrix synthesis are firmly attached to tooth surfaces. Enhanced understanding of the physical properties of mature biofilms may lead to improved approaches to detaching or disassembling these highly organized and adhesive structures. Here, the mechanical stability of S. mutans biofilms was investigated by determining their ability to withstand measured applications of shear stress using a custom-built device. The data show that the initial biofilm bulk (~ 50% biomass) was removed after exposure to 0.184 and 0.449 N m−2 for 67 and 115 h old biofilms. However, removal of the remaining biofilm close to the surface was significantly reduced (vs initial bulk removal) even when shear forces were increased 10-fold. Treatment of biofilms with exopolysaccharide-digesting dextranase substantially compromised their mechanical stability and rigidity, resulting in bulk removal at a shear stress as low as 0.027 N m−2 and > a two-fold reduction in the storage modulus (G′). The data reveal how incremental increases in shear stress cause distinctive patterns of biofilm detachment, while demonstrating that the exopolysaccharide matrix modulates the resistance of biofilms to mechanical clearance.Center for Oral Biology, University of Rochester Medical CenterDepartment of Microbiology and Immunology, University of Rochester Medical CenterBiofilm Research Labs, Levy Center for Oral Health, Department of Orthodontics, School of Dental Medicine, University of PennsylvaniaDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, Univ Estadual Paulista, UNESPDepartment of Dental Materials and Prosthodontics, Araraquara Dental School, Univ Estadual Paulista, UNESPCenter for Oral Biology, University of Rochester Medical CenterBiofilm Research Labs, Levy Center for Oral Health, Department of Orthodontics, School of Dental Medicine, University of PennsylvaniaUniversidade Estadual Paulista (Unesp)Hwang, GeelsuKlein, Marlise I. [UNESP]Koo, Hyun2018-12-11T16:56:44Z2018-12-11T16:56:44Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1079-1091application/pdfhttp://dx.doi.org/10.1080/08927014.2014.969249Biofouling, v. 30, n. 9, p. 1079-1091, 2014.1029-24540892-7014http://hdl.handle.net/11449/17171710.1080/08927014.2014.9692492-s2.0-849119825852-s2.0-84911982585.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2023-12-16T06:17:19Zoai:repositorio.unesp.br:11449/171717Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:40.515709Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
title |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
spellingShingle |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces Hwang, Geelsu dextranase exopolysaccharide matrix mechanical stability rheometry S. mutans biofilm shear stress |
title_short |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
title_full |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
title_fullStr |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
title_full_unstemmed |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
title_sort |
Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces |
author |
Hwang, Geelsu |
author_facet |
Hwang, Geelsu Klein, Marlise I. [UNESP] Koo, Hyun |
author_role |
author |
author2 |
Klein, Marlise I. [UNESP] Koo, Hyun |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Center for Oral Biology, University of Rochester Medical Center Biofilm Research Labs, Levy Center for Oral Health, Department of Orthodontics, School of Dental Medicine, University of Pennsylvania Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hwang, Geelsu Klein, Marlise I. [UNESP] Koo, Hyun |
dc.subject.por.fl_str_mv |
dextranase exopolysaccharide matrix mechanical stability rheometry S. mutans biofilm shear stress |
topic |
dextranase exopolysaccharide matrix mechanical stability rheometry S. mutans biofilm shear stress |
description |
Well-established biofilms formed by Streptococcus mutans via exopolysaccharide matrix synthesis are firmly attached to tooth surfaces. Enhanced understanding of the physical properties of mature biofilms may lead to improved approaches to detaching or disassembling these highly organized and adhesive structures. Here, the mechanical stability of S. mutans biofilms was investigated by determining their ability to withstand measured applications of shear stress using a custom-built device. The data show that the initial biofilm bulk (~ 50% biomass) was removed after exposure to 0.184 and 0.449 N m−2 for 67 and 115 h old biofilms. However, removal of the remaining biofilm close to the surface was significantly reduced (vs initial bulk removal) even when shear forces were increased 10-fold. Treatment of biofilms with exopolysaccharide-digesting dextranase substantially compromised their mechanical stability and rigidity, resulting in bulk removal at a shear stress as low as 0.027 N m−2 and > a two-fold reduction in the storage modulus (G′). The data reveal how incremental increases in shear stress cause distinctive patterns of biofilm detachment, while demonstrating that the exopolysaccharide matrix modulates the resistance of biofilms to mechanical clearance. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2018-12-11T16:56:44Z 2018-12-11T16:56:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2014.969249 Biofouling, v. 30, n. 9, p. 1079-1091, 2014. 1029-2454 0892-7014 http://hdl.handle.net/11449/171717 10.1080/08927014.2014.969249 2-s2.0-84911982585 2-s2.0-84911982585.pdf |
url |
http://dx.doi.org/10.1080/08927014.2014.969249 http://hdl.handle.net/11449/171717 |
identifier_str_mv |
Biofouling, v. 30, n. 9, p. 1079-1091, 2014. 1029-2454 0892-7014 10.1080/08927014.2014.969249 2-s2.0-84911982585 2-s2.0-84911982585.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1079-1091 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129205430386688 |