Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4236/ae.2014.22013 http://hdl.handle.net/11449/132986 |
Resumo: | Several studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28˚C ± 2˚C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment. |
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Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculationHaematobia irritansHorn FlyHatched EggMethodsBreedingSeveral studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28˚C ± 2˚C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária, Araçatuba, Rua Clóvis Pestana, 793, Jardim Dona Amélia, CEP 16050680, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Apoio, Produção e Saúde Animal, Faculdade de Medicina Veterinária, Araçatuba, Rua Clóvis Pestana, 793, Jardim Dona Amélia, CEP 16050680, SP, BrasilScientific ResearchUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Lima, Luiz Gustavo Ferraz [UNESP]Beloti, Carolina Aparecida Carlin [UNESP]Perri, SÍlvia Helena Venturoli [UNESP]Kaneto, Carlos Noriyuki [UNESP]Bresciani, Katia Denise Saraiva [UNESP]Prado, Ângelo Pires do2016-01-28T16:53:01Z2016-01-28T16:53:01Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article76-80application/pdfhttp://dx.doi.org/10.4236/ae.2014.22013Advances in Entomology, v. 2, n. 2, p. 76-80, 2014.2331-1991http://hdl.handle.net/11449/13298610.4236/ae.2014.22013ISSN2331-1991-2014-02-02-76-80.pdf081083759820263474004660277046145950594366829647Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAdvances in Entomologyinfo:eu-repo/semantics/openAccess2023-11-23T06:12:23Zoai:repositorio.unesp.br:11449/132986Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-23T06:12:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
title |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
spellingShingle |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation Lima, Luiz Gustavo Ferraz [UNESP] Haematobia irritans Horn Fly Hatched Egg Methods Breeding |
title_short |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
title_full |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
title_fullStr |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
title_full_unstemmed |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
title_sort |
Raising of horn fly haematobia irritans (L.) (diptera: muscidae) in laboratory by means of egg and larva inoculation |
author |
Lima, Luiz Gustavo Ferraz [UNESP] |
author_facet |
Lima, Luiz Gustavo Ferraz [UNESP] Beloti, Carolina Aparecida Carlin [UNESP] Perri, SÍlvia Helena Venturoli [UNESP] Kaneto, Carlos Noriyuki [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Prado, Ângelo Pires do |
author_role |
author |
author2 |
Beloti, Carolina Aparecida Carlin [UNESP] Perri, SÍlvia Helena Venturoli [UNESP] Kaneto, Carlos Noriyuki [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Prado, Ângelo Pires do |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Lima, Luiz Gustavo Ferraz [UNESP] Beloti, Carolina Aparecida Carlin [UNESP] Perri, SÍlvia Helena Venturoli [UNESP] Kaneto, Carlos Noriyuki [UNESP] Bresciani, Katia Denise Saraiva [UNESP] Prado, Ângelo Pires do |
dc.subject.por.fl_str_mv |
Haematobia irritans Horn Fly Hatched Egg Methods Breeding |
topic |
Haematobia irritans Horn Fly Hatched Egg Methods Breeding |
description |
Several studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28˚C ± 2˚C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2016-01-28T16:53:01Z 2016-01-28T16:53:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4236/ae.2014.22013 Advances in Entomology, v. 2, n. 2, p. 76-80, 2014. 2331-1991 http://hdl.handle.net/11449/132986 10.4236/ae.2014.22013 ISSN2331-1991-2014-02-02-76-80.pdf 0810837598202634 7400466027704614 5950594366829647 |
url |
http://dx.doi.org/10.4236/ae.2014.22013 http://hdl.handle.net/11449/132986 |
identifier_str_mv |
Advances in Entomology, v. 2, n. 2, p. 76-80, 2014. 2331-1991 10.4236/ae.2014.22013 ISSN2331-1991-2014-02-02-76-80.pdf 0810837598202634 7400466027704614 5950594366829647 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Advances in Entomology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
76-80 application/pdf |
dc.publisher.none.fl_str_mv |
Scientific Research |
publisher.none.fl_str_mv |
Scientific Research |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965040484286464 |