Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29

Detalhes bibliográficos
Autor(a) principal: Da Silva, Gervásio Paulo
Data de Publicação: 2015
Outros Autores: De Lima, Cristian J. Bolner [UNESP], Contiero, Jonas [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cattod.2014.05.016
http://hdl.handle.net/11449/172080
Resumo: Interest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5°C, a stirrer speed of 110-180 rpm, and a glycerol concentration of 39-49 g l<sup>-1</sup>. Batch fermentation carried out at a pH of 7.0, a temperature of 35°C, a stirrer speed of 150 rpm, and a glycerol concentration of 40 g l<sup>-1</sup> produced 20.4 g 1,3-propanediol l<sup>-1</sup>, with a maximum volumetric productivity of 2.92 g l<sup>-1</sup> h<sup>-1</sup> and a yield of 0.51 g g<sup>-1</sup>. The main byproducts were acetic acid (approximately 7.0 g l<sup>-1</sup>) and formate (approximately 3.7 g l<sup>-1</sup>). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.
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spelling Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC291,3-PropanediolBiodieselFermentationGlycerolKlebsiella pneumoniae GLC29Interest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5°C, a stirrer speed of 110-180 rpm, and a glycerol concentration of 39-49 g l<sup>-1</sup>. Batch fermentation carried out at a pH of 7.0, a temperature of 35°C, a stirrer speed of 150 rpm, and a glycerol concentration of 40 g l<sup>-1</sup> produced 20.4 g 1,3-propanediol l<sup>-1</sup>, with a maximum volumetric productivity of 2.92 g l<sup>-1</sup> h<sup>-1</sup> and a yield of 0.51 g g<sup>-1</sup>. The main byproducts were acetic acid (approximately 7.0 g l<sup>-1</sup>) and formate (approximately 3.7 g l<sup>-1</sup>). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.Microorganism Biotechnology Laboratory, Education Department, Bahia State University (UNEB), BR 407, Km 127, CEP 48970-000Industrial Microbiology Laboratory, Biochemistry and Microbiology Department, Biological Sciences Institute, São Paulo State University (UNESP), Av 24A, 1515, Bela Vista, CEP 13506-900Industrial Microbiology Laboratory, Biochemistry and Microbiology Department, Biological Sciences Institute, São Paulo State University (UNESP), Av 24A, 1515, Bela Vista, CEP 13506-900Microorganism Biotechnology Laboratory, Education Department, Bahia State University (UNEB)Universidade Estadual Paulista (Unesp)Da Silva, Gervásio PauloDe Lima, Cristian J. Bolner [UNESP]Contiero, Jonas [UNESP]2018-12-11T16:58:34Z2018-12-11T16:58:34Z2015-09-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article259-266application/pdfhttp://dx.doi.org/10.1016/j.cattod.2014.05.016Catalysis Today, v. 257, n. P2, p. 259-266, 2015.0920-5861http://hdl.handle.net/11449/17208010.1016/j.cattod.2014.05.0162-s2.0-849418031702-s2.0-84941803170.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCatalysis Today1,347info:eu-repo/semantics/openAccess2024-01-21T06:28:15Zoai:repositorio.unesp.br:11449/172080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:02.672030Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
title Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
spellingShingle Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
Da Silva, Gervásio Paulo
1,3-Propanediol
Biodiesel
Fermentation
Glycerol
Klebsiella pneumoniae GLC29
title_short Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
title_full Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
title_fullStr Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
title_full_unstemmed Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
title_sort Production and productivity of 1,3-propanediol from glycerol by Klebsiella pneumoniae GLC29
author Da Silva, Gervásio Paulo
author_facet Da Silva, Gervásio Paulo
De Lima, Cristian J. Bolner [UNESP]
Contiero, Jonas [UNESP]
author_role author
author2 De Lima, Cristian J. Bolner [UNESP]
Contiero, Jonas [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Microorganism Biotechnology Laboratory, Education Department, Bahia State University (UNEB)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Da Silva, Gervásio Paulo
De Lima, Cristian J. Bolner [UNESP]
Contiero, Jonas [UNESP]
dc.subject.por.fl_str_mv 1,3-Propanediol
Biodiesel
Fermentation
Glycerol
Klebsiella pneumoniae GLC29
topic 1,3-Propanediol
Biodiesel
Fermentation
Glycerol
Klebsiella pneumoniae GLC29
description Interest in the development of the bioproduction of 1,3-propanediol, an important chemical intermediate with various industrial applications, has increased in recent years. Klebsiella pneumoniae is one of the most studied and efficient bacteria for 1,3-propanediol production from glycerol. A new isolate of K. pneumoniae was investigated using response surface methodology by central composite design for the production of 1,3-propanediol using glycerol. The effects of pH, temperature, stirrer speed, and glycerol concentration on the production and productivity of 1,3-propanediol were examined. Considering both production and productivity, the best conditions for glycerol conversion in 1,3-propanediol are: a pH range of 6.9-7.1, a temperature between 33 and 38.5°C, a stirrer speed of 110-180 rpm, and a glycerol concentration of 39-49 g l<sup>-1</sup>. Batch fermentation carried out at a pH of 7.0, a temperature of 35°C, a stirrer speed of 150 rpm, and a glycerol concentration of 40 g l<sup>-1</sup> produced 20.4 g 1,3-propanediol l<sup>-1</sup>, with a maximum volumetric productivity of 2.92 g l<sup>-1</sup> h<sup>-1</sup> and a yield of 0.51 g g<sup>-1</sup>. The main byproducts were acetic acid (approximately 7.0 g l<sup>-1</sup>) and formate (approximately 3.7 g l<sup>-1</sup>). The newly isolated K. pneumoniae GLC29 showed potential for the conversion of glycerol into 1,3-propanediol, with high production and productivity.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-18
2018-12-11T16:58:34Z
2018-12-11T16:58:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cattod.2014.05.016
Catalysis Today, v. 257, n. P2, p. 259-266, 2015.
0920-5861
http://hdl.handle.net/11449/172080
10.1016/j.cattod.2014.05.016
2-s2.0-84941803170
2-s2.0-84941803170.pdf
url http://dx.doi.org/10.1016/j.cattod.2014.05.016
http://hdl.handle.net/11449/172080
identifier_str_mv Catalysis Today, v. 257, n. P2, p. 259-266, 2015.
0920-5861
10.1016/j.cattod.2014.05.016
2-s2.0-84941803170
2-s2.0-84941803170.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Catalysis Today
1,347
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 259-266
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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