Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/ar.20863 http://hdl.handle.net/11449/17099 |
Resumo: | Here, we evaluated collagen distribution and matrix metalloproteinases (MMPs) MMP-2 and MMP-9 activities in skeletal muscle of pacu (Piaractus mesopotamicus) during juvenile and adult growth phases. Muscle samples from juvenile and adult fishes were processed by histochemistry for collagen system fibers and for gelatin-zymography for MMP-2 and MMP-9 activities analysis. Picrosirius staining revealed a myosept, endomysium, and perimysium-like structures in both growth phases and muscle types, with increased areas of collagen fibers in adults, mainly in red muscle. Reticulin staining showed that reticular fibers in the endomysium-like structure were thinner and discontinuous in the red muscle fibers. The zymography revealed clear bands of the pro-MMP-9, active-MMP-9, intermediate-MMP-2, and active-MMP-2 forms in red and white muscle in both growth phases. MMP-2 activity was more intense in juvenile than adult muscle fibers. Comparing the red and white muscle types, MMP-2 activity was significantly higher in red muscle in adult phase only. The activity of MMP-9 forms was similar in juvenile red and white muscles and in the adult red muscle, without any activity in adult white muscle. In conclusion, our results show that, in pacu, the higher activities of MMP-2 and -9 are associated with the rapid muscle growth in juvenile age and in adult fish, these activities are related with a different red and white muscle physiology. This study may contribute to the understanding muscle growth mechanisms and may also contribute to analyse red and the white muscle parameters of firmness and softness, respectively, of the commercial product. Anat Rec, 292:387-395, 2009. (C) 2009 Wiley-Liss, Inc. |
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Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phasesfishskeletal musclecollagenextracellular matrixmetalloproteinaseszymographyHere, we evaluated collagen distribution and matrix metalloproteinases (MMPs) MMP-2 and MMP-9 activities in skeletal muscle of pacu (Piaractus mesopotamicus) during juvenile and adult growth phases. Muscle samples from juvenile and adult fishes were processed by histochemistry for collagen system fibers and for gelatin-zymography for MMP-2 and MMP-9 activities analysis. Picrosirius staining revealed a myosept, endomysium, and perimysium-like structures in both growth phases and muscle types, with increased areas of collagen fibers in adults, mainly in red muscle. Reticulin staining showed that reticular fibers in the endomysium-like structure were thinner and discontinuous in the red muscle fibers. The zymography revealed clear bands of the pro-MMP-9, active-MMP-9, intermediate-MMP-2, and active-MMP-2 forms in red and white muscle in both growth phases. MMP-2 activity was more intense in juvenile than adult muscle fibers. Comparing the red and white muscle types, MMP-2 activity was significantly higher in red muscle in adult phase only. The activity of MMP-9 forms was similar in juvenile red and white muscles and in the adult red muscle, without any activity in adult white muscle. In conclusion, our results show that, in pacu, the higher activities of MMP-2 and -9 are associated with the rapid muscle growth in juvenile age and in adult fish, these activities are related with a different red and white muscle physiology. This study may contribute to the understanding muscle growth mechanisms and may also contribute to analyse red and the white muscle parameters of firmness and softness, respectively, of the commercial product. Anat Rec, 292:387-395, 2009. (C) 2009 Wiley-Liss, Inc.Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Univ Estadual Paulista, Dept Morphol, Inst Biosci, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Bioestat, Inst Biosci, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Morphol, Inst Biosci, BR-18618000 São Paulo, BrazilUniv Estadual Paulista, Dept Bioestat, Inst Biosci, BR-18618000 São Paulo, BrazilFUNDUNESP: 00333/05-DFPFUNDUNESP: 557-01-DCPWiley-lissUniversidade Estadual Paulista (Unesp)Michelin, Aline Cristina [UNESP]Justulin, Luis Antonio [UNESP]Delella, Flávia Karina [UNESP]Padovani, Carlos Roberto [UNESP]Felisbino, Sergio Luis [UNESP]Dal-Pai-Silva, Maeli [UNESP]2014-05-20T13:47:59Z2014-05-20T13:47:59Z2009-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article387-395http://dx.doi.org/10.1002/ar.20863Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 3, p. 387-395, 2009.1932-8486http://hdl.handle.net/11449/1709910.1002/ar.20863WOS:00026398340001187278970805222897263490918934874Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnatomical Record-advances In Integrative Anatomy and Evolutionary Biology1.3730,766info:eu-repo/semantics/openAccess2021-10-22T18:27:22Zoai:repositorio.unesp.br:11449/17099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:27:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
title |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
spellingShingle |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases Michelin, Aline Cristina [UNESP] fish skeletal muscle collagen extracellular matrix metalloproteinases zymography |
title_short |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
title_full |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
title_fullStr |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
title_full_unstemmed |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
title_sort |
Differential MMP-2 and MMP-9 Activity and Collagen Distribution in Skeletal Muscle from pacu (Piaractus mesopotamicus) During Juvenile and Adult Growth Phases |
author |
Michelin, Aline Cristina [UNESP] |
author_facet |
Michelin, Aline Cristina [UNESP] Justulin, Luis Antonio [UNESP] Delella, Flávia Karina [UNESP] Padovani, Carlos Roberto [UNESP] Felisbino, Sergio Luis [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author_role |
author |
author2 |
Justulin, Luis Antonio [UNESP] Delella, Flávia Karina [UNESP] Padovani, Carlos Roberto [UNESP] Felisbino, Sergio Luis [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Michelin, Aline Cristina [UNESP] Justulin, Luis Antonio [UNESP] Delella, Flávia Karina [UNESP] Padovani, Carlos Roberto [UNESP] Felisbino, Sergio Luis [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
dc.subject.por.fl_str_mv |
fish skeletal muscle collagen extracellular matrix metalloproteinases zymography |
topic |
fish skeletal muscle collagen extracellular matrix metalloproteinases zymography |
description |
Here, we evaluated collagen distribution and matrix metalloproteinases (MMPs) MMP-2 and MMP-9 activities in skeletal muscle of pacu (Piaractus mesopotamicus) during juvenile and adult growth phases. Muscle samples from juvenile and adult fishes were processed by histochemistry for collagen system fibers and for gelatin-zymography for MMP-2 and MMP-9 activities analysis. Picrosirius staining revealed a myosept, endomysium, and perimysium-like structures in both growth phases and muscle types, with increased areas of collagen fibers in adults, mainly in red muscle. Reticulin staining showed that reticular fibers in the endomysium-like structure were thinner and discontinuous in the red muscle fibers. The zymography revealed clear bands of the pro-MMP-9, active-MMP-9, intermediate-MMP-2, and active-MMP-2 forms in red and white muscle in both growth phases. MMP-2 activity was more intense in juvenile than adult muscle fibers. Comparing the red and white muscle types, MMP-2 activity was significantly higher in red muscle in adult phase only. The activity of MMP-9 forms was similar in juvenile red and white muscles and in the adult red muscle, without any activity in adult white muscle. In conclusion, our results show that, in pacu, the higher activities of MMP-2 and -9 are associated with the rapid muscle growth in juvenile age and in adult fish, these activities are related with a different red and white muscle physiology. This study may contribute to the understanding muscle growth mechanisms and may also contribute to analyse red and the white muscle parameters of firmness and softness, respectively, of the commercial product. Anat Rec, 292:387-395, 2009. (C) 2009 Wiley-Liss, Inc. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 2014-05-20T13:47:59Z 2014-05-20T13:47:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ar.20863 Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 3, p. 387-395, 2009. 1932-8486 http://hdl.handle.net/11449/17099 10.1002/ar.20863 WOS:000263983400011 8727897080522289 7263490918934874 |
url |
http://dx.doi.org/10.1002/ar.20863 http://hdl.handle.net/11449/17099 |
identifier_str_mv |
Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology. Hoboken: Wiley-liss, v. 292, n. 3, p. 387-395, 2009. 1932-8486 10.1002/ar.20863 WOS:000263983400011 8727897080522289 7263490918934874 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anatomical Record-advances In Integrative Anatomy and Evolutionary Biology 1.373 0,766 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
387-395 |
dc.publisher.none.fl_str_mv |
Wiley-liss |
publisher.none.fl_str_mv |
Wiley-liss |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964758003154944 |