Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14814/phy2.12914 http://hdl.handle.net/11449/165329 |
Resumo: | Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling. |
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Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac functionCardiac remodelingobesitysaturated high-fat diet-adrenergic pathwayObesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ Julio de Mesquita Filho, Sch Med, Dept Internal Med, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Biosci Inst, Dept Biostat, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Sch Med, Dept Internal Med, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Biosci Inst, Dept Biostat, Sao Paulo, BrazilFAPESP: 12/20725-7FAPESP: 12/16647-0Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Vileigas, Danielle F. [UNESP]Deus, Adriana F. de [UNESP]Silva, Danielle C. T. da [UNESP]Tomasi, Loreta C. de [UNESP]Campos, Dijon H. S. de [UNESP]Adorni, Caroline S. [UNESP]Oliveira, Scarlet M. de [UNESP]Sant'Ana, Paula G. [UNESP]Okoshi, Katashi [UNESP]Padovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2018-11-27T21:47:43Z2018-11-27T21:47:43Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.14814/phy2.12914Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016.2051-817Xhttp://hdl.handle.net/11449/16532910.14814/phy2.12914WOS:000384644300013WOS000384644300013.pdf1590971576309420Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiological Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:22:36Zoai:repositorio.unesp.br:11449/165329Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
title |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
spellingShingle |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function Vileigas, Danielle F. [UNESP] Cardiac remodeling obesity saturated high-fat diet -adrenergic pathway |
title_short |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
title_full |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
title_fullStr |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
title_full_unstemmed |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
title_sort |
Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function |
author |
Vileigas, Danielle F. [UNESP] |
author_facet |
Vileigas, Danielle F. [UNESP] Deus, Adriana F. de [UNESP] Silva, Danielle C. T. da [UNESP] Tomasi, Loreta C. de [UNESP] Campos, Dijon H. S. de [UNESP] Adorni, Caroline S. [UNESP] Oliveira, Scarlet M. de [UNESP] Sant'Ana, Paula G. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author_role |
author |
author2 |
Deus, Adriana F. de [UNESP] Silva, Danielle C. T. da [UNESP] Tomasi, Loreta C. de [UNESP] Campos, Dijon H. S. de [UNESP] Adorni, Caroline S. [UNESP] Oliveira, Scarlet M. de [UNESP] Sant'Ana, Paula G. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vileigas, Danielle F. [UNESP] Deus, Adriana F. de [UNESP] Silva, Danielle C. T. da [UNESP] Tomasi, Loreta C. de [UNESP] Campos, Dijon H. S. de [UNESP] Adorni, Caroline S. [UNESP] Oliveira, Scarlet M. de [UNESP] Sant'Ana, Paula G. [UNESP] Okoshi, Katashi [UNESP] Padovani, Carlos R. [UNESP] Cicogna, Antonio C. [UNESP] |
dc.subject.por.fl_str_mv |
Cardiac remodeling obesity saturated high-fat diet -adrenergic pathway |
topic |
Cardiac remodeling obesity saturated high-fat diet -adrenergic pathway |
description |
Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-11-27T21:47:43Z 2018-11-27T21:47:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14814/phy2.12914 Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016. 2051-817X http://hdl.handle.net/11449/165329 10.14814/phy2.12914 WOS:000384644300013 WOS000384644300013.pdf 1590971576309420 |
url |
http://dx.doi.org/10.14814/phy2.12914 http://hdl.handle.net/11449/165329 |
identifier_str_mv |
Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016. 2051-817X 10.14814/phy2.12914 WOS:000384644300013 WOS000384644300013.pdf 1590971576309420 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Physiological Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128125184245760 |