Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function

Detalhes bibliográficos
Autor(a) principal: Vileigas, Danielle F. [UNESP]
Data de Publicação: 2016
Outros Autores: Deus, Adriana F. de [UNESP], Silva, Danielle C. T. da [UNESP], Tomasi, Loreta C. de [UNESP], Campos, Dijon H. S. de [UNESP], Adorni, Caroline S. [UNESP], Oliveira, Scarlet M. de [UNESP], Sant'Ana, Paula G. [UNESP], Okoshi, Katashi [UNESP], Padovani, Carlos R. [UNESP], Cicogna, Antonio C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.14814/phy2.12914
http://hdl.handle.net/11449/165329
Resumo: Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling.
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spelling Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac functionCardiac remodelingobesitysaturated high-fat diet-adrenergic pathwayObesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ Julio de Mesquita Filho, Sch Med, Dept Internal Med, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Biosci Inst, Dept Biostat, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Sch Med, Dept Internal Med, Sao Paulo, BrazilSao Paulo State Univ Julio de Mesquita Filho, Biosci Inst, Dept Biostat, Sao Paulo, BrazilFAPESP: 12/20725-7FAPESP: 12/16647-0Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Vileigas, Danielle F. [UNESP]Deus, Adriana F. de [UNESP]Silva, Danielle C. T. da [UNESP]Tomasi, Loreta C. de [UNESP]Campos, Dijon H. S. de [UNESP]Adorni, Caroline S. [UNESP]Oliveira, Scarlet M. de [UNESP]Sant'Ana, Paula G. [UNESP]Okoshi, Katashi [UNESP]Padovani, Carlos R. [UNESP]Cicogna, Antonio C. [UNESP]2018-11-27T21:47:43Z2018-11-27T21:47:43Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.14814/phy2.12914Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016.2051-817Xhttp://hdl.handle.net/11449/16532910.14814/phy2.12914WOS:000384644300013WOS000384644300013.pdf1590971576309420Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhysiological Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:22:36Zoai:repositorio.unesp.br:11449/165329Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
title Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
spellingShingle Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
Vileigas, Danielle F. [UNESP]
Cardiac remodeling
obesity
saturated high-fat diet
-adrenergic pathway
title_short Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
title_full Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
title_fullStr Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
title_full_unstemmed Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
title_sort Saturated high-fat diet-induced obesity increases adenylate cyclase of myocardial -adrenergic system and does not compromise cardiac function
author Vileigas, Danielle F. [UNESP]
author_facet Vileigas, Danielle F. [UNESP]
Deus, Adriana F. de [UNESP]
Silva, Danielle C. T. da [UNESP]
Tomasi, Loreta C. de [UNESP]
Campos, Dijon H. S. de [UNESP]
Adorni, Caroline S. [UNESP]
Oliveira, Scarlet M. de [UNESP]
Sant'Ana, Paula G. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author_role author
author2 Deus, Adriana F. de [UNESP]
Silva, Danielle C. T. da [UNESP]
Tomasi, Loreta C. de [UNESP]
Campos, Dijon H. S. de [UNESP]
Adorni, Caroline S. [UNESP]
Oliveira, Scarlet M. de [UNESP]
Sant'Ana, Paula G. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Vileigas, Danielle F. [UNESP]
Deus, Adriana F. de [UNESP]
Silva, Danielle C. T. da [UNESP]
Tomasi, Loreta C. de [UNESP]
Campos, Dijon H. S. de [UNESP]
Adorni, Caroline S. [UNESP]
Oliveira, Scarlet M. de [UNESP]
Sant'Ana, Paula G. [UNESP]
Okoshi, Katashi [UNESP]
Padovani, Carlos R. [UNESP]
Cicogna, Antonio C. [UNESP]
dc.subject.por.fl_str_mv Cardiac remodeling
obesity
saturated high-fat diet
-adrenergic pathway
topic Cardiac remodeling
obesity
saturated high-fat diet
-adrenergic pathway
description Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial -adrenergic (A) system. The purpose of this study was to evaluate the cardiac function and A pathway components in myocardium of obese rats. Male Wistar rats were distributed into two groups: control (n=17; standard diet) and obese (n=17; saturated high-fat diet) fed for 33weeks. Nutritional profile and comorbidities were assessed. Cardiac structure and function was evaluated by macroscopic postmortem, echocardiographic and isolated papillary muscle analyzes. Myocardial protein expression of (1)- and (2)-adrenergic receptors, Gs protein, adenylate cyclase (AC) and protein kinase A (PKA) was performed by Western blot. Cardiac cyclic adenosine monophosphate (cAMP) levels and PKA activity were assessed by ELISA. Obese rats showed increased adiposity index (P<0.001) and several comorbidities as hypertension, glucose intolerance, insulin resistance, and dyslipidemia compared with control rats. Echocardiographic assessment revealed increased left atrium diameter (C: 4.98 +/- 0.38 vs. Ob: 5.47 +/- 0.53, P=0.024) and posterior wall shortening velocity (C: 37.1 +/- 3.6 vs. Ob: 41.8 +/- 3.8, P=0.007) in obese group. Papillary muscle evaluation indicated that baseline data and myocardial responsiveness to isoproterenol stimulation were similar between the groups. Protein expression of myocardial AC was higher in obese group than in the control (C: 1.00 +/- 0.21 vs. Ob: 1.25 +/- 0.10, P=0.025), whereas the other components were unchanged. These results suggest that saturated high-fat diet-induced obesity was not effective in triggering cardiac dysfunction and impair the beta-adrenergic signaling.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-11-27T21:47:43Z
2018-11-27T21:47:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.14814/phy2.12914
Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016.
2051-817X
http://hdl.handle.net/11449/165329
10.14814/phy2.12914
WOS:000384644300013
WOS000384644300013.pdf
1590971576309420
url http://dx.doi.org/10.14814/phy2.12914
http://hdl.handle.net/11449/165329
identifier_str_mv Physiological Reports. Hoboken: Wiley, v. 4, n. 17, 12 p., 2016.
2051-817X
10.14814/phy2.12914
WOS:000384644300013
WOS000384644300013.pdf
1590971576309420
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Physiological Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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