Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0203748 http://hdl.handle.net/11449/164637 |
Resumo: | Violacein is a violet pigment produced by Chromobacterium violaceum that possesses several functions such as antibacterial, antiviral, antifungal, and antioxidant activities. The search for potential compounds and therapies that may interfere with and modulate the gut microbial consortia without causing severe damage and increased resistance is important for the treatment of inflammatory, allergic, and metabolic diseases. The aim of the present work was to evaluate the ability of violacein to change microbial patterns in the mammalian gut by favoring certain groups over the others in order to be used as a therapy for diseases associated with changes in the intestinal microflora. To do this, we used male Wistar rats, and administered violacein orally, in low (50 mu g/ml) and high (500 mu g/ml) doses for a month. Initially, the changes in the microbial diversity were observed by DGGE analyses that showed that the violacein significantly affects the gut microbiota of the rats. Pyrosequencing of 16S rDNA was then employed using a 454 GS Titanium platform, and the results demonstrated that higher taxonomic richness was observed with the low violacein treatment group, followed by the control group and high violacein treatment group. Modulation of the microbiota at the class level was observed in the low violacein dose, where Bacilli and Clostridia (Firmicutes) were found as dominant. For the high violacein dose, Bacilli followed by Clostridia and Actinobacteria were present as the major components. Further analyses are crucial for a better understanding of how violacein affects the gut microbiome and whether this change would be beneficial to the host, providing a framework for the development of alternative treatment strategies for intestinal diseases using this compound. |
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Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiomeViolacein is a violet pigment produced by Chromobacterium violaceum that possesses several functions such as antibacterial, antiviral, antifungal, and antioxidant activities. The search for potential compounds and therapies that may interfere with and modulate the gut microbial consortia without causing severe damage and increased resistance is important for the treatment of inflammatory, allergic, and metabolic diseases. The aim of the present work was to evaluate the ability of violacein to change microbial patterns in the mammalian gut by favoring certain groups over the others in order to be used as a therapy for diseases associated with changes in the intestinal microflora. To do this, we used male Wistar rats, and administered violacein orally, in low (50 mu g/ml) and high (500 mu g/ml) doses for a month. Initially, the changes in the microbial diversity were observed by DGGE analyses that showed that the violacein significantly affects the gut microbiota of the rats. Pyrosequencing of 16S rDNA was then employed using a 454 GS Titanium platform, and the results demonstrated that higher taxonomic richness was observed with the low violacein treatment group, followed by the control group and high violacein treatment group. Modulation of the microbiota at the class level was observed in the low violacein dose, where Bacilli and Clostridia (Firmicutes) were found as dominant. For the high violacein dose, Bacilli followed by Clostridia and Actinobacteria were present as the major components. Further analyses are crucial for a better understanding of how violacein affects the gut microbiome and whether this change would be beneficial to the host, providing a framework for the development of alternative treatment strategies for intestinal diseases using this compound.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Univ Fed Rio de Janeiro, Dept Microbiol Med, Lab Biol Anaerobios, Inst Microbiol Paulo Goes, Rio De Janeiro, RJ, BrazilFundacao Oswaldo Cruz, Escola Nacl Saude Publ Sergio Arouca, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Inst Biociencias, Lab Interacao Hospedeiro Microbiota, Campus Litoral Paulista, Sao Vicente, SP, BrazilUniv Fed Rio de Janeiro, Fac Farm, Campus Macae, Macae, RJ, BrazilFac Integradas Aparicio Carvalho, Porto Velho, RO, BrazilUniv Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Rio De Janeiro, RJ, BrazilFundacao Oswaldo Cruz, Inst Nacl Ciencia & Tecnol Inovacao Doencas Popul, Ctr Desenvolvimento Tecnol Saude, Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Inst Biociencias, Lab Interacao Hospedeiro Microbiota, Campus Litoral Paulista, Sao Vicente, SP, BrazilCNPq: 160621/2012-7FAPERJ: E-26/201.398/2014Public Library ScienceUniversidade Federal do Rio de Janeiro (UFRJ)Fundacao Oswaldo CruzUniversidade Estadual Paulista (Unesp)Fac Integradas Aparicio CarvalhoPauer, HeidiPires Hardoim, Cristiane Cassiolato [UNESP]Teixeira, Felipe LopesMiranda, Karla RodriguesBarbirato, Davi da SilvaCarvalho, Denise Pires deMartha Antunes, Luis CaetanoCosta Leitao, Lvaro Augusto daLobo, Leandro AraujoCavalcanti Pilotto Domingues, Regina Maria2018-11-26T17:55:24Z2018-11-26T17:55:24Z2018-09-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://dx.doi.org/10.1371/journal.pone.0203748Plos One. San Francisco: Public Library Science, v. 13, n. 9, 21 p., 2018.1932-6203http://hdl.handle.net/11449/16463710.1371/journal.pone.0203748WOS:000444545800064WOS000444545800064.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-10-27T06:07:11Zoai:repositorio.unesp.br:11449/164637Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:07:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
title |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
spellingShingle |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome Pauer, Heidi |
title_short |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
title_full |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
title_fullStr |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
title_full_unstemmed |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
title_sort |
Impact of violacein from Chromobacterium violaceum on the mammalian gut microbiome |
author |
Pauer, Heidi |
author_facet |
Pauer, Heidi Pires Hardoim, Cristiane Cassiolato [UNESP] Teixeira, Felipe Lopes Miranda, Karla Rodrigues Barbirato, Davi da Silva Carvalho, Denise Pires de Martha Antunes, Luis Caetano Costa Leitao, Lvaro Augusto da Lobo, Leandro Araujo Cavalcanti Pilotto Domingues, Regina Maria |
author_role |
author |
author2 |
Pires Hardoim, Cristiane Cassiolato [UNESP] Teixeira, Felipe Lopes Miranda, Karla Rodrigues Barbirato, Davi da Silva Carvalho, Denise Pires de Martha Antunes, Luis Caetano Costa Leitao, Lvaro Augusto da Lobo, Leandro Araujo Cavalcanti Pilotto Domingues, Regina Maria |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Fundacao Oswaldo Cruz Universidade Estadual Paulista (Unesp) Fac Integradas Aparicio Carvalho |
dc.contributor.author.fl_str_mv |
Pauer, Heidi Pires Hardoim, Cristiane Cassiolato [UNESP] Teixeira, Felipe Lopes Miranda, Karla Rodrigues Barbirato, Davi da Silva Carvalho, Denise Pires de Martha Antunes, Luis Caetano Costa Leitao, Lvaro Augusto da Lobo, Leandro Araujo Cavalcanti Pilotto Domingues, Regina Maria |
description |
Violacein is a violet pigment produced by Chromobacterium violaceum that possesses several functions such as antibacterial, antiviral, antifungal, and antioxidant activities. The search for potential compounds and therapies that may interfere with and modulate the gut microbial consortia without causing severe damage and increased resistance is important for the treatment of inflammatory, allergic, and metabolic diseases. The aim of the present work was to evaluate the ability of violacein to change microbial patterns in the mammalian gut by favoring certain groups over the others in order to be used as a therapy for diseases associated with changes in the intestinal microflora. To do this, we used male Wistar rats, and administered violacein orally, in low (50 mu g/ml) and high (500 mu g/ml) doses for a month. Initially, the changes in the microbial diversity were observed by DGGE analyses that showed that the violacein significantly affects the gut microbiota of the rats. Pyrosequencing of 16S rDNA was then employed using a 454 GS Titanium platform, and the results demonstrated that higher taxonomic richness was observed with the low violacein treatment group, followed by the control group and high violacein treatment group. Modulation of the microbiota at the class level was observed in the low violacein dose, where Bacilli and Clostridia (Firmicutes) were found as dominant. For the high violacein dose, Bacilli followed by Clostridia and Actinobacteria were present as the major components. Further analyses are crucial for a better understanding of how violacein affects the gut microbiome and whether this change would be beneficial to the host, providing a framework for the development of alternative treatment strategies for intestinal diseases using this compound. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:55:24Z 2018-11-26T17:55:24Z 2018-09-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0203748 Plos One. San Francisco: Public Library Science, v. 13, n. 9, 21 p., 2018. 1932-6203 http://hdl.handle.net/11449/164637 10.1371/journal.pone.0203748 WOS:000444545800064 WOS000444545800064.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0203748 http://hdl.handle.net/11449/164637 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 13, n. 9, 21 p., 2018. 1932-6203 10.1371/journal.pone.0203748 WOS:000444545800064 WOS000444545800064.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos One 1,164 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
21 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
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Public Library Science |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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