Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites

Detalhes bibliográficos
Autor(a) principal: Brolazo, Eliane Melo
Data de Publicação: 2009
Outros Autores: Simões, Jose Antonio, Nader, Maria Elena Fátima, Tomás, Maria Silvina Juárez, Gregoracci, Gustavo Bueno, Marconi, Camila [UNESP]
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/225563
Resumo: PURPOSE: To identify species of lactobacillus isolated from the vaginal contents of healthy and asymptomatic women, determining the most prevalent species and characterizing them phenotypically. METHODS: Lactobacillus have been isolated in selective milieu from samples of the vaginal contents of 135 women without complaints of vaginal secretion, and with negative laboratorial diagnosis of vaginal infection, followed up at an outpatient clinic. After being identified by multiplex PCR, the isolates have been submitted to RNAr 16S gene sequencing, when necessary. They have also been evaluated concerning the production of lactic acid, H2O2, bacteriocins and the ability to adhere to epithelial cells. RESULTS: eight-three lactobacillus strains were isolated and identified, L. crispatus (30.1%), L. jensenii (26.5%), L. gasseri (22.9%) e L. vaginalis (8.4%), being the prevalent species. Only 20 of those isolates did not present H2O2 production, in detectable amounts. From the 37 strains selected for the test of adhesion to the epithelial cells, 12 presented 50 to 69% of adhesion, 10 presented 70% or more, and the remaining, little or no adhesion at all. None of the tested strains produced bacteriocins. CONCLUSIONS: The lactobacillus species more prevalent in women without vulvovaginitis, isolated in selective culture milieu and identified by molecular methods were L. crispatus, L. jensenii and L. gasseri. Besides the fact of being more prevalent, these strains also presented better production of H2O2, and reached lower pH values in the culture milieu.
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spelling Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginitesPrevalence and characterization of vaginal lactobacillus species in women at reproductive age without vulvovaginitisLactobacillus/epidemiologyLactobacillus/isolation & purificationPolymerase chain reaction/methodsVagina/microbiologyVulvovaginitisPURPOSE: To identify species of lactobacillus isolated from the vaginal contents of healthy and asymptomatic women, determining the most prevalent species and characterizing them phenotypically. METHODS: Lactobacillus have been isolated in selective milieu from samples of the vaginal contents of 135 women without complaints of vaginal secretion, and with negative laboratorial diagnosis of vaginal infection, followed up at an outpatient clinic. After being identified by multiplex PCR, the isolates have been submitted to RNAr 16S gene sequencing, when necessary. They have also been evaluated concerning the production of lactic acid, H2O2, bacteriocins and the ability to adhere to epithelial cells. RESULTS: eight-three lactobacillus strains were isolated and identified, L. crispatus (30.1%), L. jensenii (26.5%), L. gasseri (22.9%) e L. vaginalis (8.4%), being the prevalent species. Only 20 of those isolates did not present H2O2 production, in detectable amounts. From the 37 strains selected for the test of adhesion to the epithelial cells, 12 presented 50 to 69% of adhesion, 10 presented 70% or more, and the remaining, little or no adhesion at all. None of the tested strains produced bacteriocins. CONCLUSIONS: The lactobacillus species more prevalent in women without vulvovaginitis, isolated in selective culture milieu and identified by molecular methods were L. crispatus, L. jensenii and L. gasseri. Besides the fact of being more prevalent, these strains also presented better production of H2O2, and reached lower pH values in the culture milieu.Departamento de Tocoginecologia Faculdade de Ciências Médicas Universidade Estadual de Campinas - UNICAMP, Campinas (SP)Laboratório de Microbiologia Preventiva Centro de Referência para Lactobacilos - CERELA, TucumánInstituto de Biologia Universidade Estadual de Campinas - UNICAMP, Campinas (SP)Departamento de Patologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu (SP)Departamento de Patologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista 'Júlio de Mesquita Filho' - UNESP, Botucatu (SP)Universidade Estadual de Campinas (UNICAMP)Centro de Referência para Lactobacilos - CERELAUniversidade Estadual Paulista (UNESP)Brolazo, Eliane MeloSimões, Jose AntonioNader, Maria Elena FátimaTomás, Maria Silvina JuárezGregoracci, Gustavo BuenoMarconi, Camila [UNESP]2022-04-28T20:54:28Z2022-04-28T20:54:28Z2009-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article189-195Revista Brasileira de Ginecologia e Obstetricia, v. 31, n. 4, p. 189-195, 2009.0100-7203http://hdl.handle.net/11449/2255632-s2.0-68249089517Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Ginecologia e Obstetriciainfo:eu-repo/semantics/openAccess2024-09-03T13:18:23Zoai:repositorio.unesp.br:11449/225563Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
Prevalence and characterization of vaginal lactobacillus species in women at reproductive age without vulvovaginitis
title Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
spellingShingle Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
Brolazo, Eliane Melo
Lactobacillus/epidemiology
Lactobacillus/isolation & purification
Polymerase chain reaction/methods
Vagina/microbiology
Vulvovaginitis
title_short Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
title_full Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
title_fullStr Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
title_full_unstemmed Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
title_sort Prevalência e caracterização de espécies de lactobacilos vaginais em mulheres em idade reprodutiva sem vulvovaginites
author Brolazo, Eliane Melo
author_facet Brolazo, Eliane Melo
Simões, Jose Antonio
Nader, Maria Elena Fátima
Tomás, Maria Silvina Juárez
Gregoracci, Gustavo Bueno
Marconi, Camila [UNESP]
author_role author
author2 Simões, Jose Antonio
Nader, Maria Elena Fátima
Tomás, Maria Silvina Juárez
Gregoracci, Gustavo Bueno
Marconi, Camila [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Centro de Referência para Lactobacilos - CERELA
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Brolazo, Eliane Melo
Simões, Jose Antonio
Nader, Maria Elena Fátima
Tomás, Maria Silvina Juárez
Gregoracci, Gustavo Bueno
Marconi, Camila [UNESP]
dc.subject.por.fl_str_mv Lactobacillus/epidemiology
Lactobacillus/isolation & purification
Polymerase chain reaction/methods
Vagina/microbiology
Vulvovaginitis
topic Lactobacillus/epidemiology
Lactobacillus/isolation & purification
Polymerase chain reaction/methods
Vagina/microbiology
Vulvovaginitis
description PURPOSE: To identify species of lactobacillus isolated from the vaginal contents of healthy and asymptomatic women, determining the most prevalent species and characterizing them phenotypically. METHODS: Lactobacillus have been isolated in selective milieu from samples of the vaginal contents of 135 women without complaints of vaginal secretion, and with negative laboratorial diagnosis of vaginal infection, followed up at an outpatient clinic. After being identified by multiplex PCR, the isolates have been submitted to RNAr 16S gene sequencing, when necessary. They have also been evaluated concerning the production of lactic acid, H2O2, bacteriocins and the ability to adhere to epithelial cells. RESULTS: eight-three lactobacillus strains were isolated and identified, L. crispatus (30.1%), L. jensenii (26.5%), L. gasseri (22.9%) e L. vaginalis (8.4%), being the prevalent species. Only 20 of those isolates did not present H2O2 production, in detectable amounts. From the 37 strains selected for the test of adhesion to the epithelial cells, 12 presented 50 to 69% of adhesion, 10 presented 70% or more, and the remaining, little or no adhesion at all. None of the tested strains produced bacteriocins. CONCLUSIONS: The lactobacillus species more prevalent in women without vulvovaginitis, isolated in selective culture milieu and identified by molecular methods were L. crispatus, L. jensenii and L. gasseri. Besides the fact of being more prevalent, these strains also presented better production of H2O2, and reached lower pH values in the culture milieu.
publishDate 2009
dc.date.none.fl_str_mv 2009-04-01
2022-04-28T20:54:28Z
2022-04-28T20:54:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Ginecologia e Obstetricia, v. 31, n. 4, p. 189-195, 2009.
0100-7203
http://hdl.handle.net/11449/225563
2-s2.0-68249089517
identifier_str_mv Revista Brasileira de Ginecologia e Obstetricia, v. 31, n. 4, p. 189-195, 2009.
0100-7203
2-s2.0-68249089517
url http://hdl.handle.net/11449/225563
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Ginecologia e Obstetricia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 189-195
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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