Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2012.06.010 http://hdl.handle.net/11449/74672 |
Resumo: | The recovery of sperm from the epididymal cauda may be the last chance to obtain genetic material when sudden death or serious injuries occur in valuable stallions. However, the lack of technical knowledge regarding the storage and transportation of the epididymis often prevents the preservation of the sperm. Therefore, the aim of this study was to compare sperm parameters of sperm obtained immediately after orchiectomy with sperm recovered from epididymal cauda at different times after storage at 5°C and at room temperature (RT). For that, 48 stallions of different breeds were used. In group 1 (control group), eight stallions were used, and the harvest of the epididymal sperm was performed immediately after orchiectomy. In group 2, 40 stallions were used, which were divided into five groups according to the storage time of the epididymis after orchiectomy (6, 12, 18, 24, or 30 hours), making a total of eight stallions per group. One epididymis of each stallion was stored at 5°C, and the contralateral epididymis was stored at RT, both for the same period. The sperm parameters of total motility, progressive motility, progressive linear velocity, curvilinear velocity, percentage of rapid sperm, and plasma membrane integrity were evaluated in all the groups after sperm recovery, resuspension in a sperm freezing diluent, and thawing. In conclusion, the storage of the testis-epididymis complex at 5°C provided better preservation of epididymal sperm than the storage at RT, and regardless of the temperature, the progressive motility is the sperm parameter that is most sensitive to storage time. © 2013 Elsevier Inc. |
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Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal SpermCryopreservationEpididymal spermatozoaSperm viabilityStallionStorageEquidaeThe recovery of sperm from the epididymal cauda may be the last chance to obtain genetic material when sudden death or serious injuries occur in valuable stallions. However, the lack of technical knowledge regarding the storage and transportation of the epididymis often prevents the preservation of the sperm. Therefore, the aim of this study was to compare sperm parameters of sperm obtained immediately after orchiectomy with sperm recovered from epididymal cauda at different times after storage at 5°C and at room temperature (RT). For that, 48 stallions of different breeds were used. In group 1 (control group), eight stallions were used, and the harvest of the epididymal sperm was performed immediately after orchiectomy. In group 2, 40 stallions were used, which were divided into five groups according to the storage time of the epididymis after orchiectomy (6, 12, 18, 24, or 30 hours), making a total of eight stallions per group. One epididymis of each stallion was stored at 5°C, and the contralateral epididymis was stored at RT, both for the same period. The sperm parameters of total motility, progressive motility, progressive linear velocity, curvilinear velocity, percentage of rapid sperm, and plasma membrane integrity were evaluated in all the groups after sperm recovery, resuspension in a sperm freezing diluent, and thawing. In conclusion, the storage of the testis-epididymis complex at 5°C provided better preservation of epididymal sperm than the storage at RT, and regardless of the temperature, the progressive motility is the sperm parameter that is most sensitive to storage time. © 2013 Elsevier Inc.Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP, Botucatu, São PauloDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Monteiro, Gabriel Augusto [UNESP]Guasti, Priscilla Nascimento [UNESP]Rocha, Aline Silva [UNESP]Martin, Ian [UNESP]Sancler-Silva, Yamê Fabres Robaina [UNESP]Freitas Dell'Aqua, Camila P. [UNESP]Dell'aqua Junior, José Antônio [UNESP]Papa, Frederico Ozanam [UNESP]2014-05-27T11:28:34Z2014-05-27T11:28:34Z2013-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article169-173application/pdfhttp://dx.doi.org/10.1016/j.jevs.2012.06.010Journal of Equine Veterinary Science, v. 33, n. 3, p. 169-173, 2013.0737-0806http://hdl.handle.net/11449/7467210.1016/j.jevs.2012.06.010WOS:0003157634000062-s2.0-848742877182-s2.0-84874287718.pdf0640158639112242Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0.8800,390info:eu-repo/semantics/openAccess2024-01-07T06:26:12Zoai:repositorio.unesp.br:11449/74672Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-07T06:26:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
title |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
spellingShingle |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm Monteiro, Gabriel Augusto [UNESP] Cryopreservation Epididymal spermatozoa Sperm viability Stallion Storage Equidae |
title_short |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
title_full |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
title_fullStr |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
title_full_unstemmed |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
title_sort |
Effect of Storage Time and Temperature of Equine Epididymis on the Viability, Motion Parameters, and Freezability of Epididymal Sperm |
author |
Monteiro, Gabriel Augusto [UNESP] |
author_facet |
Monteiro, Gabriel Augusto [UNESP] Guasti, Priscilla Nascimento [UNESP] Rocha, Aline Silva [UNESP] Martin, Ian [UNESP] Sancler-Silva, Yamê Fabres Robaina [UNESP] Freitas Dell'Aqua, Camila P. [UNESP] Dell'aqua Junior, José Antônio [UNESP] Papa, Frederico Ozanam [UNESP] |
author_role |
author |
author2 |
Guasti, Priscilla Nascimento [UNESP] Rocha, Aline Silva [UNESP] Martin, Ian [UNESP] Sancler-Silva, Yamê Fabres Robaina [UNESP] Freitas Dell'Aqua, Camila P. [UNESP] Dell'aqua Junior, José Antônio [UNESP] Papa, Frederico Ozanam [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Monteiro, Gabriel Augusto [UNESP] Guasti, Priscilla Nascimento [UNESP] Rocha, Aline Silva [UNESP] Martin, Ian [UNESP] Sancler-Silva, Yamê Fabres Robaina [UNESP] Freitas Dell'Aqua, Camila P. [UNESP] Dell'aqua Junior, José Antônio [UNESP] Papa, Frederico Ozanam [UNESP] |
dc.subject.por.fl_str_mv |
Cryopreservation Epididymal spermatozoa Sperm viability Stallion Storage Equidae |
topic |
Cryopreservation Epididymal spermatozoa Sperm viability Stallion Storage Equidae |
description |
The recovery of sperm from the epididymal cauda may be the last chance to obtain genetic material when sudden death or serious injuries occur in valuable stallions. However, the lack of technical knowledge regarding the storage and transportation of the epididymis often prevents the preservation of the sperm. Therefore, the aim of this study was to compare sperm parameters of sperm obtained immediately after orchiectomy with sperm recovered from epididymal cauda at different times after storage at 5°C and at room temperature (RT). For that, 48 stallions of different breeds were used. In group 1 (control group), eight stallions were used, and the harvest of the epididymal sperm was performed immediately after orchiectomy. In group 2, 40 stallions were used, which were divided into five groups according to the storage time of the epididymis after orchiectomy (6, 12, 18, 24, or 30 hours), making a total of eight stallions per group. One epididymis of each stallion was stored at 5°C, and the contralateral epididymis was stored at RT, both for the same period. The sperm parameters of total motility, progressive motility, progressive linear velocity, curvilinear velocity, percentage of rapid sperm, and plasma membrane integrity were evaluated in all the groups after sperm recovery, resuspension in a sperm freezing diluent, and thawing. In conclusion, the storage of the testis-epididymis complex at 5°C provided better preservation of epididymal sperm than the storage at RT, and regardless of the temperature, the progressive motility is the sperm parameter that is most sensitive to storage time. © 2013 Elsevier Inc. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 2014-05-27T11:28:34Z 2014-05-27T11:28:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2012.06.010 Journal of Equine Veterinary Science, v. 33, n. 3, p. 169-173, 2013. 0737-0806 http://hdl.handle.net/11449/74672 10.1016/j.jevs.2012.06.010 WOS:000315763400006 2-s2.0-84874287718 2-s2.0-84874287718.pdf 0640158639112242 |
url |
http://dx.doi.org/10.1016/j.jevs.2012.06.010 http://hdl.handle.net/11449/74672 |
identifier_str_mv |
Journal of Equine Veterinary Science, v. 33, n. 3, p. 169-173, 2013. 0737-0806 10.1016/j.jevs.2012.06.010 WOS:000315763400006 2-s2.0-84874287718 2-s2.0-84874287718.pdf 0640158639112242 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Equine Veterinary Science 0.880 0,390 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
169-173 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965545775235072 |