Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-18-20881_2010_11_3_29_24.pdf http://hdl.handle.net/11449/71952 |
Resumo: | Thirty fine-needle biopsy (FNB) samples from 28 dogs subjected to surgical resection of cutaneous mast cell tumors (MCTs) were stained with Giemsa. At least 100 neoplastic cells from each cytology slide were evaluated by morphometric analysis. The parameters were: area, perimeter of the cell, cytoplasm, nucleus and circumference factor. MCTs of grade III had a mean cellular area of 231.70 μm2 ± 57.1, and grade II had a mean of 252.30 μm2 ± 55.0. Cellular perimeter was 61.20 ± 7.1 in grade II and 59.1 ± 8.6 in grade III. Cellular parameters were not statistically different between grades (p>.05). Mean nuclear area was 88.90 μm2 ± 19 in grade III and 72.30 μm2 ± 13.9 in grade II, with statistical difference between grades (P =.011). Mean nuclear perimeter was 32.40 ìm ± 3.0 in grade II and 35.70 ìm ± 4.0 in grade III, with statistical difference between grades (P =.018). Mean nuclear circumference factor was 1.0 ± 0.33 in grade II and 1.1 ± 0.28 in grade III, with no statistical difference between grades (P = 0.78). Nuclear-tocytoplasmic ratio in grade II was 0.29 ±.07 and 0.39 ±.08 in grade III, with statistical difference (P =.02). The number of binucleated and multinucleated cells and mitotic figures was significantly increased in grade III MCTs (P <.001). In conclusion, the number of mitotic figures, presence of binucleation and multinucleation, and nuclear-to-cytoplasmic ratio can help to guide a profile of MCT aggressiveness in cytologic preparations. |
id |
UNSP_40141f1a27712aef2d3ec4e3e0fa4913 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/71952 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumorsCytopathologyImage analysisMast cell tumorMorphometryCanis familiarisThirty fine-needle biopsy (FNB) samples from 28 dogs subjected to surgical resection of cutaneous mast cell tumors (MCTs) were stained with Giemsa. At least 100 neoplastic cells from each cytology slide were evaluated by morphometric analysis. The parameters were: area, perimeter of the cell, cytoplasm, nucleus and circumference factor. MCTs of grade III had a mean cellular area of 231.70 μm2 ± 57.1, and grade II had a mean of 252.30 μm2 ± 55.0. Cellular perimeter was 61.20 ± 7.1 in grade II and 59.1 ± 8.6 in grade III. Cellular parameters were not statistically different between grades (p>.05). Mean nuclear area was 88.90 μm2 ± 19 in grade III and 72.30 μm2 ± 13.9 in grade II, with statistical difference between grades (P =.011). Mean nuclear perimeter was 32.40 ìm ± 3.0 in grade II and 35.70 ìm ± 4.0 in grade III, with statistical difference between grades (P =.018). Mean nuclear circumference factor was 1.0 ± 0.33 in grade II and 1.1 ± 0.28 in grade III, with no statistical difference between grades (P = 0.78). Nuclear-tocytoplasmic ratio in grade II was 0.29 ±.07 and 0.39 ±.08 in grade III, with statistical difference (P =.02). The number of binucleated and multinucleated cells and mitotic figures was significantly increased in grade III MCTs (P <.001). In conclusion, the number of mitotic figures, presence of binucleation and multinucleation, and nuclear-to-cytoplasmic ratio can help to guide a profile of MCT aggressiveness in cytologic preparations.Department of Clinical Veterinary Medicine Faculdade de Medicina Veterinária e Zootecnia São Paulo State University, Rubião Junior, 18618-000 Botucatu, São PauloDepartment of Veterinary Surgery and Anesthesiology FMVZ Unesp, Botucatu, São PauloDepartment of Small Animal Clinical Sciences College of Veterinary Medicine Michigan State University, East Lansing, MIDepartment of Clinical Veterinary Medicine Faculdade de Medicina Veterinária e Zootecnia São Paulo State University, Rubião Junior, 18618-000 Botucatu, São PauloDepartment of Veterinary Surgery and Anesthesiology FMVZ Unesp, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Michigan State UniversityNeto, Rafael Torres [UNESP]Pinczowski, Pedro [UNESP]Rahal, Sheila Canevese [UNESP]Kitchell, Barbara E.Amorim, Renée Laufer [UNESP]2014-05-27T11:24:50Z2014-05-27T11:24:50Z2010-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article93-99http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-18-20881_2010_11_3_29_24.pdfBrazilian Journal of Veterinary Pathology, v. 3, n. 2, p. 93-99, 2010.1983-0246http://hdl.handle.net/11449/719522-s2.0-79952457689149743326539019497958290221081050000-0002-9211-4093Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T07:53:13Zoai:repositorio.unesp.br:11449/71952Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:09:32.723291Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
title |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
spellingShingle |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors Neto, Rafael Torres [UNESP] Cytopathology Image analysis Mast cell tumor Morphometry Canis familiaris |
title_short |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
title_full |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
title_fullStr |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
title_full_unstemmed |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
title_sort |
Cytoplasmic and nuclear morphometric parameters in cytologic preparations of canine cutaneous mast cell tumors |
author |
Neto, Rafael Torres [UNESP] |
author_facet |
Neto, Rafael Torres [UNESP] Pinczowski, Pedro [UNESP] Rahal, Sheila Canevese [UNESP] Kitchell, Barbara E. Amorim, Renée Laufer [UNESP] |
author_role |
author |
author2 |
Pinczowski, Pedro [UNESP] Rahal, Sheila Canevese [UNESP] Kitchell, Barbara E. Amorim, Renée Laufer [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Michigan State University |
dc.contributor.author.fl_str_mv |
Neto, Rafael Torres [UNESP] Pinczowski, Pedro [UNESP] Rahal, Sheila Canevese [UNESP] Kitchell, Barbara E. Amorim, Renée Laufer [UNESP] |
dc.subject.por.fl_str_mv |
Cytopathology Image analysis Mast cell tumor Morphometry Canis familiaris |
topic |
Cytopathology Image analysis Mast cell tumor Morphometry Canis familiaris |
description |
Thirty fine-needle biopsy (FNB) samples from 28 dogs subjected to surgical resection of cutaneous mast cell tumors (MCTs) were stained with Giemsa. At least 100 neoplastic cells from each cytology slide were evaluated by morphometric analysis. The parameters were: area, perimeter of the cell, cytoplasm, nucleus and circumference factor. MCTs of grade III had a mean cellular area of 231.70 μm2 ± 57.1, and grade II had a mean of 252.30 μm2 ± 55.0. Cellular perimeter was 61.20 ± 7.1 in grade II and 59.1 ± 8.6 in grade III. Cellular parameters were not statistically different between grades (p>.05). Mean nuclear area was 88.90 μm2 ± 19 in grade III and 72.30 μm2 ± 13.9 in grade II, with statistical difference between grades (P =.011). Mean nuclear perimeter was 32.40 ìm ± 3.0 in grade II and 35.70 ìm ± 4.0 in grade III, with statistical difference between grades (P =.018). Mean nuclear circumference factor was 1.0 ± 0.33 in grade II and 1.1 ± 0.28 in grade III, with no statistical difference between grades (P = 0.78). Nuclear-tocytoplasmic ratio in grade II was 0.29 ±.07 and 0.39 ±.08 in grade III, with statistical difference (P =.02). The number of binucleated and multinucleated cells and mitotic figures was significantly increased in grade III MCTs (P <.001). In conclusion, the number of mitotic figures, presence of binucleation and multinucleation, and nuclear-to-cytoplasmic ratio can help to guide a profile of MCT aggressiveness in cytologic preparations. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-01 2014-05-27T11:24:50Z 2014-05-27T11:24:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-18-20881_2010_11_3_29_24.pdf Brazilian Journal of Veterinary Pathology, v. 3, n. 2, p. 93-99, 2010. 1983-0246 http://hdl.handle.net/11449/71952 2-s2.0-79952457689 1497433265390194 9795829022108105 0000-0002-9211-4093 |
url |
http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-18-20881_2010_11_3_29_24.pdf http://hdl.handle.net/11449/71952 |
identifier_str_mv |
Brazilian Journal of Veterinary Pathology, v. 3, n. 2, p. 93-99, 2010. 1983-0246 2-s2.0-79952457689 1497433265390194 9795829022108105 0000-0002-9211-4093 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-99 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129495717117952 |