qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10493-018-0260-0 http://hdl.handle.net/11449/166179 |
Resumo: | Babesia spp. are tick-transmitted intraerythrocytic apicomplexan parasites that infect wild and domestic animals. Babesia bovis and B. bigemina are endemic and responsible for enormous economic losses to the livestock industry in most of the Brazilian territory, wherein the tick Rhipicephalus microplus is the unique vector. Better understanding of epidemiology and parasite-host interactions may improve the tools for disease control and genetic management for selection of resistant animals. This study aimed to detect, quantify and measure the correlation between B. bigemina and B. bovis infection levels in bovine blood and into tick, by absolute quantification of hemoparasite DNA using qPCR. Blood bovine samples and larvae pools from 10 engorged R. microplus females were collected from each Canchim heifers (5/8 Charolais + 3/8 zebu, n = 36). All evaluated samples were positive for both Babesia species tested. Correlations of B. bovis and B. bigemina levels between cattle and tick host were 0.58 and 0.66, respectively. These high positive correlation coefficients indicate that parasitemia load in the bovine may be dependent on or may determine the parasitemia load in the ticks. |
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qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvaeCorrelationInfectionBabesiosisCattleTicksBabesia spp. are tick-transmitted intraerythrocytic apicomplexan parasites that infect wild and domestic animals. Babesia bovis and B. bigemina are endemic and responsible for enormous economic losses to the livestock industry in most of the Brazilian territory, wherein the tick Rhipicephalus microplus is the unique vector. Better understanding of epidemiology and parasite-host interactions may improve the tools for disease control and genetic management for selection of resistant animals. This study aimed to detect, quantify and measure the correlation between B. bigemina and B. bovis infection levels in bovine blood and into tick, by absolute quantification of hemoparasite DNA using qPCR. Blood bovine samples and larvae pools from 10 engorged R. microplus females were collected from each Canchim heifers (5/8 Charolais + 3/8 zebu, n = 36). All evaluated samples were positive for both Babesia species tested. Correlations of B. bovis and B. bigemina levels between cattle and tick host were 0.58 and 0.66, respectively. These high positive correlation coefficients indicate that parasitemia load in the bovine may be dependent on or may determine the parasitemia load in the ticks.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Agricultural Research CorporationUniv Estadual Julio de Mesquita Filho, Unesp Jaboticabal, Sao Paulo, BrazilEmbrapa Pecuaria Sudeste, Rodovia Washington Luiz,Km 234,CP 339, BR-13560970 Sao Paulo, BrazilUniv Estadual Julio de Mesquita Filho, Unesp Jaboticabal, Sao Paulo, BrazilFAPESP: 2013/20513-5FAPESP: 2016/07216-7Brazilian Agricultural Research Corporation: SEG 02.12.02.008.00.00SpringerUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Giglioti, Rodrigo [UNESP]Oliveira, Henrique Nunes de [UNESP]Okino, Cintia HiromiSena Oliveira, Marcia Cristina de2018-11-29T17:54:06Z2018-11-29T17:54:06Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article235-240application/pdfhttp://dx.doi.org/10.1007/s10493-018-0260-0Experimental And Applied Acarology. Dordrecht: Springer, v. 75, n. 2, p. 235-240, 2018.0168-8162http://hdl.handle.net/11449/16617910.1007/s10493-018-0260-0WOS:000434262500007WOS000434262500007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental And Applied Acarologyinfo:eu-repo/semantics/openAccess2023-12-14T06:20:59Zoai:repositorio.unesp.br:11449/166179Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-14T06:20:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
title |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
spellingShingle |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae Giglioti, Rodrigo [UNESP] Correlation Infection Babesiosis Cattle Ticks |
title_short |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
title_full |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
title_fullStr |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
title_full_unstemmed |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
title_sort |
qPCR estimates of Babesia bovis and Babesia bigemina infection levels in beef cattle and Rhipicephalus microplus larvae |
author |
Giglioti, Rodrigo [UNESP] |
author_facet |
Giglioti, Rodrigo [UNESP] Oliveira, Henrique Nunes de [UNESP] Okino, Cintia Hiromi Sena Oliveira, Marcia Cristina de |
author_role |
author |
author2 |
Oliveira, Henrique Nunes de [UNESP] Okino, Cintia Hiromi Sena Oliveira, Marcia Cristina de |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Giglioti, Rodrigo [UNESP] Oliveira, Henrique Nunes de [UNESP] Okino, Cintia Hiromi Sena Oliveira, Marcia Cristina de |
dc.subject.por.fl_str_mv |
Correlation Infection Babesiosis Cattle Ticks |
topic |
Correlation Infection Babesiosis Cattle Ticks |
description |
Babesia spp. are tick-transmitted intraerythrocytic apicomplexan parasites that infect wild and domestic animals. Babesia bovis and B. bigemina are endemic and responsible for enormous economic losses to the livestock industry in most of the Brazilian territory, wherein the tick Rhipicephalus microplus is the unique vector. Better understanding of epidemiology and parasite-host interactions may improve the tools for disease control and genetic management for selection of resistant animals. This study aimed to detect, quantify and measure the correlation between B. bigemina and B. bovis infection levels in bovine blood and into tick, by absolute quantification of hemoparasite DNA using qPCR. Blood bovine samples and larvae pools from 10 engorged R. microplus females were collected from each Canchim heifers (5/8 Charolais + 3/8 zebu, n = 36). All evaluated samples were positive for both Babesia species tested. Correlations of B. bovis and B. bigemina levels between cattle and tick host were 0.58 and 0.66, respectively. These high positive correlation coefficients indicate that parasitemia load in the bovine may be dependent on or may determine the parasitemia load in the ticks. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-29T17:54:06Z 2018-11-29T17:54:06Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10493-018-0260-0 Experimental And Applied Acarology. Dordrecht: Springer, v. 75, n. 2, p. 235-240, 2018. 0168-8162 http://hdl.handle.net/11449/166179 10.1007/s10493-018-0260-0 WOS:000434262500007 WOS000434262500007.pdf |
url |
http://dx.doi.org/10.1007/s10493-018-0260-0 http://hdl.handle.net/11449/166179 |
identifier_str_mv |
Experimental And Applied Acarology. Dordrecht: Springer, v. 75, n. 2, p. 235-240, 2018. 0168-8162 10.1007/s10493-018-0260-0 WOS:000434262500007 WOS000434262500007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental And Applied Acarology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
235-240 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965283147841536 |