MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/226880 |
Resumo: | Bladder carcinoma is one of the most common tumors in the world and, despite the therapy currently available, most of the patients relapse. Better understanding of the factors involved in disease pathogenesis would provide insights for the development of more effective strategies in treatment. Recently, differential miRNA expression profiles in bladder urothelial carcinomas identified miR-100 down-regulation and miR-708 up-regulation among the most common alterations, although the possible influence of these miRNAs in the control of basic mechanisms in bladder tumors has not been addressed. In this context, the present study aimed to evaluate the in vitro effects of miR-100 forced expression and miR-708 inhibition in the bladder carcinoma cell line 5637. Our results showed that overexpression of miR-100 significantly inhibited growth when compared to controls at both times tested (72 and 96 hours, p<0.01) with a maximum effect at 72 hours reducing proliferation in 29.6 %. Conversely, no effects on cell growth were observed after inhibition of miR-708. MiR-100 also reduced colony formation capacity of 5637 cells by 24.4%. No alterations in cell cycle progression or apoptosis induction were observed. The effects of miR-100 on growth and clonogenicity capacity in 5637 cells evince a possible role of this miRNA in bladder carcinoma pathogenesis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future therapeutic interventions. |
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MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cellsBladder carcinomaMicroRNA-100Tumor suppressorBladder carcinoma is one of the most common tumors in the world and, despite the therapy currently available, most of the patients relapse. Better understanding of the factors involved in disease pathogenesis would provide insights for the development of more effective strategies in treatment. Recently, differential miRNA expression profiles in bladder urothelial carcinomas identified miR-100 down-regulation and miR-708 up-regulation among the most common alterations, although the possible influence of these miRNAs in the control of basic mechanisms in bladder tumors has not been addressed. In this context, the present study aimed to evaluate the in vitro effects of miR-100 forced expression and miR-708 inhibition in the bladder carcinoma cell line 5637. Our results showed that overexpression of miR-100 significantly inhibited growth when compared to controls at both times tested (72 and 96 hours, p<0.01) with a maximum effect at 72 hours reducing proliferation in 29.6 %. Conversely, no effects on cell growth were observed after inhibition of miR-708. MiR-100 also reduced colony formation capacity of 5637 cells by 24.4%. No alterations in cell cycle progression or apoptosis induction were observed. The effects of miR-100 on growth and clonogenicity capacity in 5637 cells evince a possible role of this miRNA in bladder carcinoma pathogenesis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future therapeutic interventions.Department of Genetics Faculty of Medicine of Ribeirão Preto University of São PauloDivision of Pediatric Oncology Department of Pediatrics Faculty of Medicine of Ribeirão Preto University of São PauloDepartment of Pathology Faculty of Medicine of Botucatu São Paulo State University - UNESPDepartment of Pathology Faculty of Medicine of Botucatu São Paulo State University - UNESPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Oliveira, Jaqueline C.Brassesco, María S.Morales, Andressa G.Pezuk, Julia A.Fedatto, Paola Fernandada Silva, Glenda N. [UNESP]Scrideli, Carlos A.Tone, Luiz G.2022-04-29T03:58:12Z2022-04-29T03:58:12Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3001-3004Asian Pacific Journal of Cancer Prevention, v. 12, n. 11, p. 3001-3004, 2011.2476-762X1513-7368http://hdl.handle.net/11449/2268802-s2.0-84863318483Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAsian Pacific Journal of Cancer Preventioninfo:eu-repo/semantics/openAccess2024-09-03T13:18:14Zoai:repositorio.unesp.br:11449/226880Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
title |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
spellingShingle |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells Oliveira, Jaqueline C. Bladder carcinoma MicroRNA-100 Tumor suppressor |
title_short |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
title_full |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
title_fullStr |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
title_full_unstemmed |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
title_sort |
MicroRNA-100 acts as a tumor suppressor in human bladder carcinoma 5637 cells |
author |
Oliveira, Jaqueline C. |
author_facet |
Oliveira, Jaqueline C. Brassesco, María S. Morales, Andressa G. Pezuk, Julia A. Fedatto, Paola Fernanda da Silva, Glenda N. [UNESP] Scrideli, Carlos A. Tone, Luiz G. |
author_role |
author |
author2 |
Brassesco, María S. Morales, Andressa G. Pezuk, Julia A. Fedatto, Paola Fernanda da Silva, Glenda N. [UNESP] Scrideli, Carlos A. Tone, Luiz G. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Jaqueline C. Brassesco, María S. Morales, Andressa G. Pezuk, Julia A. Fedatto, Paola Fernanda da Silva, Glenda N. [UNESP] Scrideli, Carlos A. Tone, Luiz G. |
dc.subject.por.fl_str_mv |
Bladder carcinoma MicroRNA-100 Tumor suppressor |
topic |
Bladder carcinoma MicroRNA-100 Tumor suppressor |
description |
Bladder carcinoma is one of the most common tumors in the world and, despite the therapy currently available, most of the patients relapse. Better understanding of the factors involved in disease pathogenesis would provide insights for the development of more effective strategies in treatment. Recently, differential miRNA expression profiles in bladder urothelial carcinomas identified miR-100 down-regulation and miR-708 up-regulation among the most common alterations, although the possible influence of these miRNAs in the control of basic mechanisms in bladder tumors has not been addressed. In this context, the present study aimed to evaluate the in vitro effects of miR-100 forced expression and miR-708 inhibition in the bladder carcinoma cell line 5637. Our results showed that overexpression of miR-100 significantly inhibited growth when compared to controls at both times tested (72 and 96 hours, p<0.01) with a maximum effect at 72 hours reducing proliferation in 29.6 %. Conversely, no effects on cell growth were observed after inhibition of miR-708. MiR-100 also reduced colony formation capacity of 5637 cells by 24.4%. No alterations in cell cycle progression or apoptosis induction were observed. The effects of miR-100 on growth and clonogenicity capacity in 5637 cells evince a possible role of this miRNA in bladder carcinoma pathogenesis. Further studies are necessary to corroborate our findings and examine the potential use of this microRNA in future therapeutic interventions. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 2022-04-29T03:58:12Z 2022-04-29T03:58:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Asian Pacific Journal of Cancer Prevention, v. 12, n. 11, p. 3001-3004, 2011. 2476-762X 1513-7368 http://hdl.handle.net/11449/226880 2-s2.0-84863318483 |
identifier_str_mv |
Asian Pacific Journal of Cancer Prevention, v. 12, n. 11, p. 3001-3004, 2011. 2476-762X 1513-7368 2-s2.0-84863318483 |
url |
http://hdl.handle.net/11449/226880 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Asian Pacific Journal of Cancer Prevention |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3001-3004 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021407347703808 |