Avaliação do processo de reparo alveolar em ratos portadores de diabetes tipo 2, induzido por dieta hiperlipídica e baixa dose de estreptozotocina: análise morfométrica, imunoistoquímica e por microscopia de birrefringência

• Autor(a) principal: Palin, Letícia Pitol [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/203418; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-05-29/000915170.pdf
• topic: Diabetes mellitus; Dieta hiperlipídica; Estreptozocina; Alvéolo dental; Densidade óssea; Ratos; Diet, High-Fat

Type I diabetes interferes with bone metabolism, delaying alveolar bone repair, but little is known about the influence of type II disease in the alveolar repair process after the dental extraction. Objective: To analyze the dynamics of alveolar bone repair in type II diabetic rats by immunohistochemical, birefringence, microtomographic and confocal microscopy analysis. Fifty rats (n = 25 per group) were used, divided into two groups: Control (CO) and Diabetic type II (DBT-2). The animals in the DBT-2 group had diabetes induced by caffeteria diet and a single application of streptozotocin (35mg/kg). After the verification of type II diabetes, the animals were submitted the surgery of dental extraction and euthanasia after 3, 7, 14 and 42 days respectively. For the immunohistochemical analysis, antibodies against TNFα, TGFβ, IL6 (inflammatory response at 3 and 7 days) and TRAP, OC, WNT (bone repair at 14 and 42 days) were used. For the birefringence analysis, the sections stained with Picrosirius-red were analyzed to evaluate the organization and quantity of the collagen fibers at 14 and 42 days after the dental extraction. For Micro-CT analysis (42 days) the parameters evaluated were BV/TV, Po.Tot, Tb.Th, Tb.N and Tb.Sp. For confocal microscopy analysis, the parameters used were bone tissue dynamics and active mineralization surface. The quantitative data were submitted to a statistical analysis and a significance level of 5% was adopted. Results: In the immunohistochemical analysis, at 3 days, the CO group presented a light staining for IL-6, TNFα and TGFβ while at 7 days, presented light staining for IL-6, and moderate for TNFα and TGFβ. In the DBT-2 group, at 3 days the markers for IL-6, TNFα and TGFβ were moderate whereas at 7 days, there was intense staining for IL-6 and TGF and moderate for TNFα. At 14 days, the CO group presented moderate staining...