Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11010-016-2753-7 http://hdl.handle.net/11449/168773 |
Resumo: | Parkinsonia aculeata L. (Caesalpiniaceae) is a traditional ethnomedicine and has been used for the empiric treatment of hyperglycemia, without scientific background. Mechanistic analyses at molecular level from the antioxidant mechanism observed by P. aculeata are required. Herein the effects of the treatment by hydroethanolic extract partitioned with ethyl acetate of P. aculeata aerial parts (HEPa/EtOAc) in mice fed a high-fat diet that share many obesity phenotypes with humans were evaluated. The animals were treated orally with HEPa/EtOAc (125 and 250 mg/kg/day) and pioglitazone (5 mg/kg/day), for 16 days. After the treatment, HEPa/EtOAc reduced fasting serum glucose and insulin levels, as well as homeostasis model assessment for insulin resistance. In addition, an improvement in glucose intolerance was also observed. Indeed, a reduction in the circulating levels of TNF-α and IL-6 was also observed. Furthermore, at molecular level, it was demonstrated that the HEPa/EtOAc treatment was able to improve these physiological parameters, through the activation of peroxisome proliferator-activated receptor γ (PPARγ) per si, as well as the enhancement of antioxidant mechanism by an increase in PPARγ/Cu2+, Zn2+-superoxide dismutase (CuZn-SOD) axis expression in liver and adipose tissue. In sum, P. aculeata is effective to improve insulin resistance in a mouse model of obesity and this effect seems to involve the antioxidant and anti-inflammatory mechanisms through the increase in PPARγ/CuZn-SOD axis expression. |
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Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity miceAntioxidantCuZn-SODFabaceaeInsulin resistanceObesityOxidative stressParkinsonia aculeataPPARγParkinsonia aculeata L. (Caesalpiniaceae) is a traditional ethnomedicine and has been used for the empiric treatment of hyperglycemia, without scientific background. Mechanistic analyses at molecular level from the antioxidant mechanism observed by P. aculeata are required. Herein the effects of the treatment by hydroethanolic extract partitioned with ethyl acetate of P. aculeata aerial parts (HEPa/EtOAc) in mice fed a high-fat diet that share many obesity phenotypes with humans were evaluated. The animals were treated orally with HEPa/EtOAc (125 and 250 mg/kg/day) and pioglitazone (5 mg/kg/day), for 16 days. After the treatment, HEPa/EtOAc reduced fasting serum glucose and insulin levels, as well as homeostasis model assessment for insulin resistance. In addition, an improvement in glucose intolerance was also observed. Indeed, a reduction in the circulating levels of TNF-α and IL-6 was also observed. Furthermore, at molecular level, it was demonstrated that the HEPa/EtOAc treatment was able to improve these physiological parameters, through the activation of peroxisome proliferator-activated receptor γ (PPARγ) per si, as well as the enhancement of antioxidant mechanism by an increase in PPARγ/Cu2+, Zn2+-superoxide dismutase (CuZn-SOD) axis expression in liver and adipose tissue. In sum, P. aculeata is effective to improve insulin resistance in a mouse model of obesity and this effect seems to involve the antioxidant and anti-inflammatory mechanisms through the increase in PPARγ/CuZn-SOD axis expression.Department of Physiology and Pharmacology Federal University of Pernambuco (UFPE) Cidade UniversitáriaDepartment of Internal Medicine State University of CampinasDepartment of Physical Education São Paulo State University (UNESP)Department of Physical Education São Paulo State University (UNESP)Universidade Federal de Pernambuco (UFPE)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Araújo, Tiago GomesOliveira, Alexandre Gabarra [UNESP]Vecina, Juliana FalcatoMarin, Rodrigo MiguelFranco, Eryvelton SouzaAbdalla Saad, Mario J.de Sousa Maia, Maria Bernadete2018-12-11T16:42:59Z2018-12-11T16:42:59Z2016-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article93-101application/pdfhttp://dx.doi.org/10.1007/s11010-016-2753-7Molecular and Cellular Biochemistry, v. 419, n. 1-2, p. 93-101, 2016.1573-49190300-8177http://hdl.handle.net/11449/16877310.1007/s11010-016-2753-72-s2.0-849764770392-s2.0-84976477039.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Biochemistry1,0031,003info:eu-repo/semantics/openAccess2023-11-17T06:12:56Zoai:repositorio.unesp.br:11449/168773Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:58:08.662370Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
title |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
spellingShingle |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice Araújo, Tiago Gomes Antioxidant CuZn-SOD Fabaceae Insulin resistance Obesity Oxidative stress Parkinsonia aculeata PPARγ |
title_short |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
title_full |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
title_fullStr |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
title_full_unstemmed |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
title_sort |
Treatment with Parkinsonia aculeata combats insulin resistance-induced oxidative stress through the increase in PPARγ/CuZn-SOD axis expression in diet-induced obesity mice |
author |
Araújo, Tiago Gomes |
author_facet |
Araújo, Tiago Gomes Oliveira, Alexandre Gabarra [UNESP] Vecina, Juliana Falcato Marin, Rodrigo Miguel Franco, Eryvelton Souza Abdalla Saad, Mario J. de Sousa Maia, Maria Bernadete |
author_role |
author |
author2 |
Oliveira, Alexandre Gabarra [UNESP] Vecina, Juliana Falcato Marin, Rodrigo Miguel Franco, Eryvelton Souza Abdalla Saad, Mario J. de Sousa Maia, Maria Bernadete |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Pernambuco (UFPE) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Araújo, Tiago Gomes Oliveira, Alexandre Gabarra [UNESP] Vecina, Juliana Falcato Marin, Rodrigo Miguel Franco, Eryvelton Souza Abdalla Saad, Mario J. de Sousa Maia, Maria Bernadete |
dc.subject.por.fl_str_mv |
Antioxidant CuZn-SOD Fabaceae Insulin resistance Obesity Oxidative stress Parkinsonia aculeata PPARγ |
topic |
Antioxidant CuZn-SOD Fabaceae Insulin resistance Obesity Oxidative stress Parkinsonia aculeata PPARγ |
description |
Parkinsonia aculeata L. (Caesalpiniaceae) is a traditional ethnomedicine and has been used for the empiric treatment of hyperglycemia, without scientific background. Mechanistic analyses at molecular level from the antioxidant mechanism observed by P. aculeata are required. Herein the effects of the treatment by hydroethanolic extract partitioned with ethyl acetate of P. aculeata aerial parts (HEPa/EtOAc) in mice fed a high-fat diet that share many obesity phenotypes with humans were evaluated. The animals were treated orally with HEPa/EtOAc (125 and 250 mg/kg/day) and pioglitazone (5 mg/kg/day), for 16 days. After the treatment, HEPa/EtOAc reduced fasting serum glucose and insulin levels, as well as homeostasis model assessment for insulin resistance. In addition, an improvement in glucose intolerance was also observed. Indeed, a reduction in the circulating levels of TNF-α and IL-6 was also observed. Furthermore, at molecular level, it was demonstrated that the HEPa/EtOAc treatment was able to improve these physiological parameters, through the activation of peroxisome proliferator-activated receptor γ (PPARγ) per si, as well as the enhancement of antioxidant mechanism by an increase in PPARγ/Cu2+, Zn2+-superoxide dismutase (CuZn-SOD) axis expression in liver and adipose tissue. In sum, P. aculeata is effective to improve insulin resistance in a mouse model of obesity and this effect seems to involve the antioxidant and anti-inflammatory mechanisms through the increase in PPARγ/CuZn-SOD axis expression. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-01 2018-12-11T16:42:59Z 2018-12-11T16:42:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11010-016-2753-7 Molecular and Cellular Biochemistry, v. 419, n. 1-2, p. 93-101, 2016. 1573-4919 0300-8177 http://hdl.handle.net/11449/168773 10.1007/s11010-016-2753-7 2-s2.0-84976477039 2-s2.0-84976477039.pdf |
url |
http://dx.doi.org/10.1007/s11010-016-2753-7 http://hdl.handle.net/11449/168773 |
identifier_str_mv |
Molecular and Cellular Biochemistry, v. 419, n. 1-2, p. 93-101, 2016. 1573-4919 0300-8177 10.1007/s11010-016-2753-7 2-s2.0-84976477039 2-s2.0-84976477039.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular and Cellular Biochemistry 1,003 1,003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
93-101 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128879751069696 |