Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0177103 http://hdl.handle.net/11449/159535 |
Resumo: | Uncaria tomentosa (Willd. ex Schult.) DC., a plant native to the Amazon region, is used widely in popular medicine and by the pharmaceutical industry because of its anti-inflammatory activity. However, the survival of this species is endangered by deforestation and indiscriminate collection, and a preservation plan is urgently required. The objectives of this study were to determine the genetic and chemical variability between and within eight populations of U. tomentosa from the Brazilian states of Acre, Para and Amapa, and to investigate possible correlations between genetic and geographical distances, and between geographical distances or altitude and the accumulation of bioactive oxindole alkaloids. Three sequence-related amplified polymorphism (SRAP) markers were employed to fingerprint genomic DNA, and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high-performance liquid chromatography. Although significant divergence existed between the tested populations (F-ST = 0.246), the largest genetic diversity and the highest percentage of polymorphism (95.68%) was found within the population from Mancio Lima, Acre. Gene flow was considered rather limited (Nm = 1.57), and no correlations between genetic and geographical distances were detected, suggesting that population structure followed an island model. Accumulations of mitraphylline and isomitraphylline varied in the range 32.94 to 0.57 and 3.75 to 0.36 mg g(-1) dry weight, respectively. The concentration of isomitraphylline was positively influenced by altitude, such that the population collected at the site with the highest elevation (Tarauaca, Acre) exhibited the greatest alkaloid content. SRAP markers were very efficient in fingerprinting genomic DNA from U. tomentosa populations and clearly showed that genetic variability within populations was greater than between populations. A conservation and management plan should prioritize the creation of germplasm banks to prevent the loss of existing genetic variability, particularly within alkaloid-rich populations such as those of Tarauaca |
id |
UNSP_40af63f8b52fabc7aa0089aa708754cd |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/159535 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian AmazonUncaria tomentosa (Willd. ex Schult.) DC., a plant native to the Amazon region, is used widely in popular medicine and by the pharmaceutical industry because of its anti-inflammatory activity. However, the survival of this species is endangered by deforestation and indiscriminate collection, and a preservation plan is urgently required. The objectives of this study were to determine the genetic and chemical variability between and within eight populations of U. tomentosa from the Brazilian states of Acre, Para and Amapa, and to investigate possible correlations between genetic and geographical distances, and between geographical distances or altitude and the accumulation of bioactive oxindole alkaloids. Three sequence-related amplified polymorphism (SRAP) markers were employed to fingerprint genomic DNA, and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high-performance liquid chromatography. Although significant divergence existed between the tested populations (F-ST = 0.246), the largest genetic diversity and the highest percentage of polymorphism (95.68%) was found within the population from Mancio Lima, Acre. Gene flow was considered rather limited (Nm = 1.57), and no correlations between genetic and geographical distances were detected, suggesting that population structure followed an island model. Accumulations of mitraphylline and isomitraphylline varied in the range 32.94 to 0.57 and 3.75 to 0.36 mg g(-1) dry weight, respectively. The concentration of isomitraphylline was positively influenced by altitude, such that the population collected at the site with the highest elevation (Tarauaca, Acre) exhibited the greatest alkaloid content. SRAP markers were very efficient in fingerprinting genomic DNA from U. tomentosa populations and clearly showed that genetic variability within populations was greater than between populations. A conservation and management plan should prioritize the creation of germplasm banks to prevent the loss of existing genetic variability, particularly within alkaloid-rich populations such as those of TarauacaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Campus Lageado, Botucatu, SP, BrazilUniv Ribeirao Preto, Unidade Biotecnol, Ribeirao Preto, SP, BrazilPontificia Univ Catolica Goias, Escola Ciencias Agr & Biol, Goiania, Go, BrazilUniv Fed Goias, Lab Genet & Biodiversidade, Goiania, Go, BrazilReserva EcoCerrado Brasil, Araxa, MG, BrazilUniv Fed Goias, Dept Ecol, ICB, Goiania, Go, BrazilUniv Estadual Paulista, Campus Lageado, Botucatu, SP, BrazilCNPq: 405167/2013-0Public Library ScienceUniversidade Estadual Paulista (Unesp)Univ Ribeirao PretoPontificia Univ Catolica GoiasUniversidade Federal de Goiás (UFG)Reserva EcoCerrado BrasilGomes Honorio, Isabela Cristina [UNESP]Bertoni, Bianca WaleriaCampos Telles, Mariana Pires deBraga, Ramilla dos SantosFranca, Suzelei de CastroCoppede, Juliana da SilvaConde Correa, Valeria SieroFelizola Diniz Filho, Jose AlexandreSoares Pereira, Ana Maria2018-11-26T15:44:11Z2018-11-26T15:44:11Z2017-05-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://dx.doi.org/10.1371/journal.pone.0177103Plos One. San Francisco: Public Library Science, v. 12, n. 5, 17 p., 2017.1932-6203http://hdl.handle.net/11449/15953510.1371/journal.pone.0177103WOS:000400649500048WOS000400649500048.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2024-01-04T06:21:28Zoai:repositorio.unesp.br:11449/159535Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:12.295229Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
title |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
spellingShingle |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon Gomes Honorio, Isabela Cristina [UNESP] |
title_short |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
title_full |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
title_fullStr |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
title_full_unstemmed |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
title_sort |
Genetic and chemical diversity of Uncaria tomentosa (Willd. ex. Schult.) DC. in the Brazilian Amazon |
author |
Gomes Honorio, Isabela Cristina [UNESP] |
author_facet |
Gomes Honorio, Isabela Cristina [UNESP] Bertoni, Bianca Waleria Campos Telles, Mariana Pires de Braga, Ramilla dos Santos Franca, Suzelei de Castro Coppede, Juliana da Silva Conde Correa, Valeria Siero Felizola Diniz Filho, Jose Alexandre Soares Pereira, Ana Maria |
author_role |
author |
author2 |
Bertoni, Bianca Waleria Campos Telles, Mariana Pires de Braga, Ramilla dos Santos Franca, Suzelei de Castro Coppede, Juliana da Silva Conde Correa, Valeria Siero Felizola Diniz Filho, Jose Alexandre Soares Pereira, Ana Maria |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Ribeirao Preto Pontificia Univ Catolica Goias Universidade Federal de Goiás (UFG) Reserva EcoCerrado Brasil |
dc.contributor.author.fl_str_mv |
Gomes Honorio, Isabela Cristina [UNESP] Bertoni, Bianca Waleria Campos Telles, Mariana Pires de Braga, Ramilla dos Santos Franca, Suzelei de Castro Coppede, Juliana da Silva Conde Correa, Valeria Siero Felizola Diniz Filho, Jose Alexandre Soares Pereira, Ana Maria |
description |
Uncaria tomentosa (Willd. ex Schult.) DC., a plant native to the Amazon region, is used widely in popular medicine and by the pharmaceutical industry because of its anti-inflammatory activity. However, the survival of this species is endangered by deforestation and indiscriminate collection, and a preservation plan is urgently required. The objectives of this study were to determine the genetic and chemical variability between and within eight populations of U. tomentosa from the Brazilian states of Acre, Para and Amapa, and to investigate possible correlations between genetic and geographical distances, and between geographical distances or altitude and the accumulation of bioactive oxindole alkaloids. Three sequence-related amplified polymorphism (SRAP) markers were employed to fingerprint genomic DNA, and the amounts of mitraphylline and isomitraphylline in leaf samples were established by high-performance liquid chromatography. Although significant divergence existed between the tested populations (F-ST = 0.246), the largest genetic diversity and the highest percentage of polymorphism (95.68%) was found within the population from Mancio Lima, Acre. Gene flow was considered rather limited (Nm = 1.57), and no correlations between genetic and geographical distances were detected, suggesting that population structure followed an island model. Accumulations of mitraphylline and isomitraphylline varied in the range 32.94 to 0.57 and 3.75 to 0.36 mg g(-1) dry weight, respectively. The concentration of isomitraphylline was positively influenced by altitude, such that the population collected at the site with the highest elevation (Tarauaca, Acre) exhibited the greatest alkaloid content. SRAP markers were very efficient in fingerprinting genomic DNA from U. tomentosa populations and clearly showed that genetic variability within populations was greater than between populations. A conservation and management plan should prioritize the creation of germplasm banks to prevent the loss of existing genetic variability, particularly within alkaloid-rich populations such as those of Tarauaca |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-05 2018-11-26T15:44:11Z 2018-11-26T15:44:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0177103 Plos One. San Francisco: Public Library Science, v. 12, n. 5, 17 p., 2017. 1932-6203 http://hdl.handle.net/11449/159535 10.1371/journal.pone.0177103 WOS:000400649500048 WOS000400649500048.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0177103 http://hdl.handle.net/11449/159535 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 12, n. 5, 17 p., 2017. 1932-6203 10.1371/journal.pone.0177103 WOS:000400649500048 WOS000400649500048.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129388815843328 |