Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/14114 http://hdl.handle.net/11449/40140 |
Resumo: | The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 degrees C for 30 min and kept at -196 degrees C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 degrees C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 degrees C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 degrees C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
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Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectantCriopreservação de Agaricus blazei em nitrogênio líquido usando dmso como crioprotetorAgaricus brasiliensisAgaricus subrufescensThawingFreezingViabilityPreservationThe preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 degrees C for 30 min and kept at -196 degrees C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 degrees C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 degrees C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 degrees C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Paranaense UNIPAR, Lab Biol Mol, Umuarama, PR, BrazilUniv Estadual Paulista UNESP, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Botucatu, SP, BrazilUniversidade Federal de Uberlândia (UFU)Univ Paranaense UNIPARUniversidade Estadual Paulista (Unesp)Colauto, Nelson Barrosda Eira, Augusto Ferreira [UNESP]Linde, Giani Andrea2014-05-20T15:30:50Z2014-05-20T15:30:50Z2012-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1034-1037application/pdfhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/14114Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 28, n. 6, p. 1034-1037, 2012.1516-3725http://hdl.handle.net/11449/40140WOS:000317382600025WOS000317382600025.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioscience Journal0,303info:eu-repo/semantics/openAccess2023-10-27T06:08:57Zoai:repositorio.unesp.br:11449/40140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:08:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant Criopreservação de Agaricus blazei em nitrogênio líquido usando dmso como crioprotetor |
title |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
spellingShingle |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant Colauto, Nelson Barros Agaricus brasiliensis Agaricus subrufescens Thawing Freezing Viability Preservation |
title_short |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
title_full |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
title_fullStr |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
title_full_unstemmed |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
title_sort |
Cryopreservation of Agaricus blazei in liquid nitrogen using dmso as cryoprotectant |
author |
Colauto, Nelson Barros |
author_facet |
Colauto, Nelson Barros da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
author_role |
author |
author2 |
da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Univ Paranaense UNIPAR Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Colauto, Nelson Barros da Eira, Augusto Ferreira [UNESP] Linde, Giani Andrea |
dc.subject.por.fl_str_mv |
Agaricus brasiliensis Agaricus subrufescens Thawing Freezing Viability Preservation |
topic |
Agaricus brasiliensis Agaricus subrufescens Thawing Freezing Viability Preservation |
description |
The preservation of Agaricus blazei is generally done using successive subcultivations that are laborious and are subject to contaminations or genetic degenerations, resulting in loss of biotechnological interest characteristics. An alternative process would be cryopreservation, but there are no reports of methodologies for this basidiomycete in liquid nitrogen. Thus, the objective of this study was to evaluate mycelial viability of A. blazei strains after cryopreservation in liquid nitrogen in order to establish the initial parameters of species preservation. Five strains grown on malt extract agar (MEA) were used. Disks of MEA containing A. blazei mycelium were transferred for screw-cap cryovials containing the cryoprotectant, 10% dimethyl sulfoxide. Then, they were cooled at 8 degrees C for 30 min and kept at -196 degrees C with liquid nitrogen. After 1.5 year of cryopreservation, the cryovials were thawed in water bath at 30 degrees C for 15 min. The disks with mycelia were transferred to MEA culture media without cryoprotectant and kept at 28 degrees C for 30 days. A. blazei strains respond differently to the cryopreservation method at -196 degrees C by varying mycelial viability recovery. Cryopreservation with liquid nitrogen, using dimethyl sulfoxide as cryoprotectant, is not the most appropriate one for A. blazei preservation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-01 2014-05-20T15:30:50Z 2014-05-20T15:30:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.seer.ufu.br/index.php/biosciencejournal/article/view/14114 Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 28, n. 6, p. 1034-1037, 2012. 1516-3725 http://hdl.handle.net/11449/40140 WOS:000317382600025 WOS000317382600025.pdf |
url |
http://www.seer.ufu.br/index.php/biosciencejournal/article/view/14114 http://hdl.handle.net/11449/40140 |
identifier_str_mv |
Bioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 28, n. 6, p. 1034-1037, 2012. 1516-3725 WOS:000317382600025 WOS000317382600025.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioscience Journal 0,303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1034-1037 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) |
publisher.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964720447356928 |