Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous)
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Publication Date: | 2020 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.theriogenology.2020.06.016 http://hdl.handle.net/11449/200670 |
Summary: | Our objective was to condition adult male crab-eating fox (Cerdocyon thous) to allow semen collection by digital manipulation of the penis, assess sperm characteristics, and use ultrasonography to assess and measure testes and prostate and determine echotexture, as well as hemodynamics of testicular and spermatic cord arteries. Five captive C. thous (2–5 y, ∼7 kg) were used. After conditioning for digital sperm collection, 13 ejaculates were obtained from two males. Ejaculate characteristics (mean ± SD) were volume (393.2 ± 178.0 μL), concentration (463.7 ± 594.4 × 106 sperm/mL), total sperm number (217.4 ± 304.0 × 106 sperm), total motility (68.0 ± 6.1%), progressive motility (48.8 ± 5.1%) and rapid sperm (57.2 ± 6.8%). The most prevalent sperm morphological defects were abnormal acrosome, small abnormal head, and diadem defect. Under chemical restraint, testes and prostate were evaluated with B-mode and spectral Doppler ultrasonography. Based on ultrasonography, testicular volumes were (2.2 ± 0.7 and 2.1 ± 0.7 cm3 for left and right testes, respectively), whereas prostate dimensions were 2.3 ± 0.4 cm long and 1.5 ± 0.3 cm thick. Lastly, using triplex Doppler, peak systolic velocity (PSV) was higher in testicular versus intratesticular arteries. In conclusion, adult male C. thous were conditioned to allow sperm collection by digital manipulation without urine contamination, enabling evaluation of sperm characteristics. Furthermore, B-mode ultrasound facilitated evaluation of testes and prostate morphometry and echotexture, whereas Doppler was used to characterize testicular blood flow. This was proof of concept for semen collection and baseline data for future male reproductive soundness examinations and development of assisted reproductive techniques in this species. |
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Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous)Animal reproductionMale wild canidaeReproductive ultrasonographySemen collectionSperm morphologyOur objective was to condition adult male crab-eating fox (Cerdocyon thous) to allow semen collection by digital manipulation of the penis, assess sperm characteristics, and use ultrasonography to assess and measure testes and prostate and determine echotexture, as well as hemodynamics of testicular and spermatic cord arteries. Five captive C. thous (2–5 y, ∼7 kg) were used. After conditioning for digital sperm collection, 13 ejaculates were obtained from two males. Ejaculate characteristics (mean ± SD) were volume (393.2 ± 178.0 μL), concentration (463.7 ± 594.4 × 106 sperm/mL), total sperm number (217.4 ± 304.0 × 106 sperm), total motility (68.0 ± 6.1%), progressive motility (48.8 ± 5.1%) and rapid sperm (57.2 ± 6.8%). The most prevalent sperm morphological defects were abnormal acrosome, small abnormal head, and diadem defect. Under chemical restraint, testes and prostate were evaluated with B-mode and spectral Doppler ultrasonography. Based on ultrasonography, testicular volumes were (2.2 ± 0.7 and 2.1 ± 0.7 cm3 for left and right testes, respectively), whereas prostate dimensions were 2.3 ± 0.4 cm long and 1.5 ± 0.3 cm thick. Lastly, using triplex Doppler, peak systolic velocity (PSV) was higher in testicular versus intratesticular arteries. In conclusion, adult male C. thous were conditioned to allow sperm collection by digital manipulation without urine contamination, enabling evaluation of sperm characteristics. Furthermore, B-mode ultrasound facilitated evaluation of testes and prostate morphometry and echotexture, whereas Doppler was used to characterize testicular blood flow. This was proof of concept for semen collection and baseline data for future male reproductive soundness examinations and development of assisted reproductive techniques in this species.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Production Animal Health Faculty of Veterinary Medicine University of CalgaryDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)CAPES: 001Universidade Estadual Paulista (Unesp)University of CalgaryCarvalho, Jaqueline Candido de [UNESP]Silva, Fernando Evaristo da [UNESP]Rizzoto, GuilhermeDadalto, Carmell Rezende [UNESP]Rolim, Luna Scarpari [UNESP]Mamprim, Maria Jaqueline [UNESP]Souza, Fabiana Ferreira de [UNESP]Teixeira, Carlos Roberto [UNESP]Kastelic, John PatrickFerreira, João Carlos Pinheiro [UNESP]2020-12-12T02:12:57Z2020-12-12T02:12:57Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article60-69http://dx.doi.org/10.1016/j.theriogenology.2020.06.016Theriogenology, v. 155, p. 60-69.0093-691Xhttp://hdl.handle.net/11449/20067010.1016/j.theriogenology.2020.06.0162-s2.0-85087120816Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2021-10-23T12:24:07Zoai:repositorio.unesp.br:11449/200670Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T12:24:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
title |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
spellingShingle |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) Carvalho, Jaqueline Candido de [UNESP] Animal reproduction Male wild canidae Reproductive ultrasonography Semen collection Sperm morphology |
title_short |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
title_full |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
title_fullStr |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
title_full_unstemmed |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
title_sort |
Semen collection, sperm characteristics and ultrasonographic features of reproductive tissues in crab-eating fox (Cerdocyon thous) |
author |
Carvalho, Jaqueline Candido de [UNESP] |
author_facet |
Carvalho, Jaqueline Candido de [UNESP] Silva, Fernando Evaristo da [UNESP] Rizzoto, Guilherme Dadalto, Carmell Rezende [UNESP] Rolim, Luna Scarpari [UNESP] Mamprim, Maria Jaqueline [UNESP] Souza, Fabiana Ferreira de [UNESP] Teixeira, Carlos Roberto [UNESP] Kastelic, John Patrick Ferreira, João Carlos Pinheiro [UNESP] |
author_role |
author |
author2 |
Silva, Fernando Evaristo da [UNESP] Rizzoto, Guilherme Dadalto, Carmell Rezende [UNESP] Rolim, Luna Scarpari [UNESP] Mamprim, Maria Jaqueline [UNESP] Souza, Fabiana Ferreira de [UNESP] Teixeira, Carlos Roberto [UNESP] Kastelic, John Patrick Ferreira, João Carlos Pinheiro [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Calgary |
dc.contributor.author.fl_str_mv |
Carvalho, Jaqueline Candido de [UNESP] Silva, Fernando Evaristo da [UNESP] Rizzoto, Guilherme Dadalto, Carmell Rezende [UNESP] Rolim, Luna Scarpari [UNESP] Mamprim, Maria Jaqueline [UNESP] Souza, Fabiana Ferreira de [UNESP] Teixeira, Carlos Roberto [UNESP] Kastelic, John Patrick Ferreira, João Carlos Pinheiro [UNESP] |
dc.subject.por.fl_str_mv |
Animal reproduction Male wild canidae Reproductive ultrasonography Semen collection Sperm morphology |
topic |
Animal reproduction Male wild canidae Reproductive ultrasonography Semen collection Sperm morphology |
description |
Our objective was to condition adult male crab-eating fox (Cerdocyon thous) to allow semen collection by digital manipulation of the penis, assess sperm characteristics, and use ultrasonography to assess and measure testes and prostate and determine echotexture, as well as hemodynamics of testicular and spermatic cord arteries. Five captive C. thous (2–5 y, ∼7 kg) were used. After conditioning for digital sperm collection, 13 ejaculates were obtained from two males. Ejaculate characteristics (mean ± SD) were volume (393.2 ± 178.0 μL), concentration (463.7 ± 594.4 × 106 sperm/mL), total sperm number (217.4 ± 304.0 × 106 sperm), total motility (68.0 ± 6.1%), progressive motility (48.8 ± 5.1%) and rapid sperm (57.2 ± 6.8%). The most prevalent sperm morphological defects were abnormal acrosome, small abnormal head, and diadem defect. Under chemical restraint, testes and prostate were evaluated with B-mode and spectral Doppler ultrasonography. Based on ultrasonography, testicular volumes were (2.2 ± 0.7 and 2.1 ± 0.7 cm3 for left and right testes, respectively), whereas prostate dimensions were 2.3 ± 0.4 cm long and 1.5 ± 0.3 cm thick. Lastly, using triplex Doppler, peak systolic velocity (PSV) was higher in testicular versus intratesticular arteries. In conclusion, adult male C. thous were conditioned to allow sperm collection by digital manipulation without urine contamination, enabling evaluation of sperm characteristics. Furthermore, B-mode ultrasound facilitated evaluation of testes and prostate morphometry and echotexture, whereas Doppler was used to characterize testicular blood flow. This was proof of concept for semen collection and baseline data for future male reproductive soundness examinations and development of assisted reproductive techniques in this species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:12:57Z 2020-12-12T02:12:57Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2020.06.016 Theriogenology, v. 155, p. 60-69. 0093-691X http://hdl.handle.net/11449/200670 10.1016/j.theriogenology.2020.06.016 2-s2.0-85087120816 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2020.06.016 http://hdl.handle.net/11449/200670 |
identifier_str_mv |
Theriogenology, v. 155, p. 60-69. 0093-691X 10.1016/j.theriogenology.2020.06.016 2-s2.0-85087120816 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
60-69 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789981524099072 |