Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scirp.org/Journal/PaperInformation.aspx?PaperID=43240#.VUvMdY7BwXA http://hdl.handle.net/11449/123619 |
Resumo: | Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil metagenomic libraries. Soil environment has immense diversity of microorganisms, yet most remains unexplored and the metagenome are the technologies used and investigate uncultured microorganisms potential. The aim is to identify novel genes using the metagenomic approaches from a bioinformatics perspective and hopefully will serve as a useful resource. With this purpose, we used the metagenomic library of Eucalyptus spp. arboretum (EAA). We did a screening to select a positive clone and submitted them to the process of shotgun. The data obtained was submitted to bioinformatics analyses. These analyses identified were the novel MetaPPase gene and were classified according to the predict family and subfamily. |
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Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily predictionSoil DiversityBioinformaticStructureMembrane ProteinIon Transportmetagenômicapirofosfatasetranslocador de H+Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil metagenomic libraries. Soil environment has immense diversity of microorganisms, yet most remains unexplored and the metagenome are the technologies used and investigate uncultured microorganisms potential. The aim is to identify novel genes using the metagenomic approaches from a bioinformatics perspective and hopefully will serve as a useful resource. With this purpose, we used the metagenomic library of Eucalyptus spp. arboretum (EAA). We did a screening to select a positive clone and submitted them to the process of shotgun. The data obtained was submitted to bioinformatics analyses. These analyses identified were the novel MetaPPase gene and were classified according to the predict family and subfamily.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Tecnologia, Faculadade de Ciências Agrárias e Veterinárias Jaboticabal, Jaboticabal, Via deAcesso Prof Paulo Donato Castellani s/n, Rural, CEP 14884-900, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Tecnologia, Faculadade de Ciências Agrárias e Veterinárias de JaboticabalUniversidade Estadual Paulista (Unesp)Rodrigues, Gisele Regina [UNESP]Val-Moraes, Silvana Pompeia [UNESP]Lemos, Eliana Gertrudes Macedo [UNESP]Pizauro Júnior, Joao Martins [UNESP]2015-05-15T13:30:30Z2015-05-15T13:30:30Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article68-75application/pdfhttp://www.scirp.org/Journal/PaperInformation.aspx?PaperID=43240#.VUvMdY7BwXAOpen Journal of Applied Sciences, v. 4, n. 2, p. 68-75, 2014.2165-3917http://hdl.handle.net/11449/12361910.4236/ojapps.2014.42008ISSN2165-3917-2014-04-02-68-75.pdf395812449847909039020209364809434462378046178590Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Journal of Applied Sciencesinfo:eu-repo/semantics/openAccess2023-10-28T06:08:26Zoai:repositorio.unesp.br:11449/123619Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-28T06:08:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
title |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
spellingShingle |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction Rodrigues, Gisele Regina [UNESP] Soil Diversity Bioinformatic Structure Membrane Protein Ion Transport metagenômica pirofosfatase translocador de H+ |
title_short |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
title_full |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
title_fullStr |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
title_full_unstemmed |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
title_sort |
Novel inorganic pyrophosphatase from soil metagenomic and family and subfamily prediction |
author |
Rodrigues, Gisele Regina [UNESP] |
author_facet |
Rodrigues, Gisele Regina [UNESP] Val-Moraes, Silvana Pompeia [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Pizauro Júnior, Joao Martins [UNESP] |
author_role |
author |
author2 |
Val-Moraes, Silvana Pompeia [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Pizauro Júnior, Joao Martins [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rodrigues, Gisele Regina [UNESP] Val-Moraes, Silvana Pompeia [UNESP] Lemos, Eliana Gertrudes Macedo [UNESP] Pizauro Júnior, Joao Martins [UNESP] |
dc.subject.por.fl_str_mv |
Soil Diversity Bioinformatic Structure Membrane Protein Ion Transport metagenômica pirofosfatase translocador de H+ |
topic |
Soil Diversity Bioinformatic Structure Membrane Protein Ion Transport metagenômica pirofosfatase translocador de H+ |
description |
Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil metagenomic libraries. Soil environment has immense diversity of microorganisms, yet most remains unexplored and the metagenome are the technologies used and investigate uncultured microorganisms potential. The aim is to identify novel genes using the metagenomic approaches from a bioinformatics perspective and hopefully will serve as a useful resource. With this purpose, we used the metagenomic library of Eucalyptus spp. arboretum (EAA). We did a screening to select a positive clone and submitted them to the process of shotgun. The data obtained was submitted to bioinformatics analyses. These analyses identified were the novel MetaPPase gene and were classified according to the predict family and subfamily. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-05-15T13:30:30Z 2015-05-15T13:30:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scirp.org/Journal/PaperInformation.aspx?PaperID=43240#.VUvMdY7BwXA Open Journal of Applied Sciences, v. 4, n. 2, p. 68-75, 2014. 2165-3917 http://hdl.handle.net/11449/123619 10.4236/ojapps.2014.42008 ISSN2165-3917-2014-04-02-68-75.pdf 3958124498479090 3902020936480943 4462378046178590 |
url |
http://www.scirp.org/Journal/PaperInformation.aspx?PaperID=43240#.VUvMdY7BwXA http://hdl.handle.net/11449/123619 |
identifier_str_mv |
Open Journal of Applied Sciences, v. 4, n. 2, p. 68-75, 2014. 2165-3917 10.4236/ojapps.2014.42008 ISSN2165-3917-2014-04-02-68-75.pdf 3958124498479090 3902020936480943 4462378046178590 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Open Journal of Applied Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
68-75 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964732786999296 |