Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer

Detalhes bibliográficos
Autor(a) principal: Piccin-Santos, Viviane [UNESP]
Data de Publicação: 2014
Outros Autores: Brandão, Marcelo Mendes, Bittencourt-Oliveira, Maria Do Carmo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/jpy.12204
http://hdl.handle.net/11449/227840
Resumo: Selection of genes that have not been horizontally transferred for prokaryote phylogenetic inferences is regarded as a challenging task. The markers internal transcribed spacer of ribosomal genes (16S-23S ITS) and phycocyanin intergenic spacer (PC-IGS), based on the operons of ribosomal and phycocyanin genes respectively, are among the most used markers in cyanobacteria. The region of the ribosomal genes has been considered stable, whereas the phycocyanin operon may have undergone horizontal transfer. To investigate the occurrence of horizontal transfer of PC-IGS, phylogenetic trees of Geitlerinema and Microcystis strains were generated using PC-IGS and 16S-23S ITS and compared. Phylogenetic trees based on the two markers were mostly congruent for Geitlerinema and Microcystis, indicating a common evolutionary history among ribosomal and phycocyanin genes with no evidence for horizontal transfer of PC-IGS. Thus, PC-IGS is a suitable marker, along with 16S-23S ITS for phylogenetic studies of cyanobacteria. © 2014 Phycological Society of America.
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spelling Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer16S-23S ITSCyanobacteriaLateral gene transferMolecular phylogenyPhycocyaninRibosomal genesSelection of genes that have not been horizontally transferred for prokaryote phylogenetic inferences is regarded as a challenging task. The markers internal transcribed spacer of ribosomal genes (16S-23S ITS) and phycocyanin intergenic spacer (PC-IGS), based on the operons of ribosomal and phycocyanin genes respectively, are among the most used markers in cyanobacteria. The region of the ribosomal genes has been considered stable, whereas the phycocyanin operon may have undergone horizontal transfer. To investigate the occurrence of horizontal transfer of PC-IGS, phylogenetic trees of Geitlerinema and Microcystis strains were generated using PC-IGS and 16S-23S ITS and compared. Phylogenetic trees based on the two markers were mostly congruent for Geitlerinema and Microcystis, indicating a common evolutionary history among ribosomal and phycocyanin genes with no evidence for horizontal transfer of PC-IGS. Thus, PC-IGS is a suitable marker, along with 16S-23S ITS for phylogenetic studies of cyanobacteria. © 2014 Phycological Society of America.Department of Biological Sciences, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo 13418-900Institute of Biological Sciences, São Paulo State University, Rio Claro, São Paulo 13506-900Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA - UNICAMP), Campinas, São Paulo, 13081-970Department of Genetics, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, São Paulo, 13418-900Institute of Biological Sciences, São Paulo State University, Rio Claro, São Paulo 13506-900Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)Piccin-Santos, Viviane [UNESP]Brandão, Marcelo MendesBittencourt-Oliveira, Maria Do Carmo2022-04-29T07:20:25Z2022-04-29T07:20:25Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article736-743http://dx.doi.org/10.1111/jpy.12204Journal of Phycology, v. 50, n. 4, p. 736-743, 2014.1529-88170022-3646http://hdl.handle.net/11449/22784010.1111/jpy.122042-s2.0-84905273704Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Phycologyinfo:eu-repo/semantics/openAccess2022-04-29T07:20:25Zoai:repositorio.unesp.br:11449/227840Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:08:32.570273Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
title Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
spellingShingle Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
Piccin-Santos, Viviane [UNESP]
16S-23S ITS
Cyanobacteria
Lateral gene transfer
Molecular phylogeny
Phycocyanin
Ribosomal genes
title_short Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
title_full Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
title_fullStr Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
title_full_unstemmed Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
title_sort Phylogenetic study of Geitlerinema and Microcystis (Cyanobacteria) using PC-IGS and 16S-23S ITS as markers: Investigation of horizontal gene transfer
author Piccin-Santos, Viviane [UNESP]
author_facet Piccin-Santos, Viviane [UNESP]
Brandão, Marcelo Mendes
Bittencourt-Oliveira, Maria Do Carmo
author_role author
author2 Brandão, Marcelo Mendes
Bittencourt-Oliveira, Maria Do Carmo
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Piccin-Santos, Viviane [UNESP]
Brandão, Marcelo Mendes
Bittencourt-Oliveira, Maria Do Carmo
dc.subject.por.fl_str_mv 16S-23S ITS
Cyanobacteria
Lateral gene transfer
Molecular phylogeny
Phycocyanin
Ribosomal genes
topic 16S-23S ITS
Cyanobacteria
Lateral gene transfer
Molecular phylogeny
Phycocyanin
Ribosomal genes
description Selection of genes that have not been horizontally transferred for prokaryote phylogenetic inferences is regarded as a challenging task. The markers internal transcribed spacer of ribosomal genes (16S-23S ITS) and phycocyanin intergenic spacer (PC-IGS), based on the operons of ribosomal and phycocyanin genes respectively, are among the most used markers in cyanobacteria. The region of the ribosomal genes has been considered stable, whereas the phycocyanin operon may have undergone horizontal transfer. To investigate the occurrence of horizontal transfer of PC-IGS, phylogenetic trees of Geitlerinema and Microcystis strains were generated using PC-IGS and 16S-23S ITS and compared. Phylogenetic trees based on the two markers were mostly congruent for Geitlerinema and Microcystis, indicating a common evolutionary history among ribosomal and phycocyanin genes with no evidence for horizontal transfer of PC-IGS. Thus, PC-IGS is a suitable marker, along with 16S-23S ITS for phylogenetic studies of cyanobacteria. © 2014 Phycological Society of America.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2022-04-29T07:20:25Z
2022-04-29T07:20:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/jpy.12204
Journal of Phycology, v. 50, n. 4, p. 736-743, 2014.
1529-8817
0022-3646
http://hdl.handle.net/11449/227840
10.1111/jpy.12204
2-s2.0-84905273704
url http://dx.doi.org/10.1111/jpy.12204
http://hdl.handle.net/11449/227840
identifier_str_mv Journal of Phycology, v. 50, n. 4, p. 736-743, 2014.
1529-8817
0022-3646
10.1111/jpy.12204
2-s2.0-84905273704
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Phycology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 736-743
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128900960616448

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