The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-35982011001000010 http://hdl.handle.net/11449/2599 |
Resumo: | Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. |
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The effects of ovalbumin as a protein source during the in vitro production of bovine embryosbovine embryoin vitro cultureOvalbuminProtein sourceEmbryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP FCAV Departamento de Reprodução AnimalUSP Faculdade de Zootecnia e Engenharia de AlimentosEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuária SudesteUNICAMP Instituto de Química Departamento de Química OrgânicaUNESP FCAV Departamento de Reprodução AnimalSociedade Brasileira de ZootecniaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual de Campinas (UNICAMP)Tetzner, Tatiane Almeida Drummond [UNESP]Saraiva, Naiara Zoccal [UNESP]Perecin, FelipeNiciura, Simone Cristina MéoFerreira, Christina RamiresOliveira, Clara Slade [UNESP]Garcia, Joaquim Mansano [UNESP]2014-05-20T13:15:28Z2014-05-20T13:15:28Z2011-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2135-2141application/pdfhttp://dx.doi.org/10.1590/S1516-35982011001000010Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, 2011.1516-3598http://hdl.handle.net/11449/259910.1590/S1516-35982011001000010S1516-35982011001000010WOS:000297341400010S1516-35982011001000010.pdf2251116139872527SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Zootecnia0,337info:eu-repo/semantics/openAccess2024-06-06T18:10:12Zoai:repositorio.unesp.br:11449/2599Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:28:02.794717Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
title |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
spellingShingle |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos Tetzner, Tatiane Almeida Drummond [UNESP] bovine embryo in vitro culture Ovalbumin Protein source |
title_short |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
title_full |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
title_fullStr |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
title_full_unstemmed |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
title_sort |
The effects of ovalbumin as a protein source during the in vitro production of bovine embryos |
author |
Tetzner, Tatiane Almeida Drummond [UNESP] |
author_facet |
Tetzner, Tatiane Almeida Drummond [UNESP] Saraiva, Naiara Zoccal [UNESP] Perecin, Felipe Niciura, Simone Cristina Méo Ferreira, Christina Ramires Oliveira, Clara Slade [UNESP] Garcia, Joaquim Mansano [UNESP] |
author_role |
author |
author2 |
Saraiva, Naiara Zoccal [UNESP] Perecin, Felipe Niciura, Simone Cristina Méo Ferreira, Christina Ramires Oliveira, Clara Slade [UNESP] Garcia, Joaquim Mansano [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Tetzner, Tatiane Almeida Drummond [UNESP] Saraiva, Naiara Zoccal [UNESP] Perecin, Felipe Niciura, Simone Cristina Méo Ferreira, Christina Ramires Oliveira, Clara Slade [UNESP] Garcia, Joaquim Mansano [UNESP] |
dc.subject.por.fl_str_mv |
bovine embryo in vitro culture Ovalbumin Protein source |
topic |
bovine embryo in vitro culture Ovalbumin Protein source |
description |
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-01 2014-05-20T13:15:28Z 2014-05-20T13:15:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-35982011001000010 Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, 2011. 1516-3598 http://hdl.handle.net/11449/2599 10.1590/S1516-35982011001000010 S1516-35982011001000010 WOS:000297341400010 S1516-35982011001000010.pdf 2251116139872527 |
url |
http://dx.doi.org/10.1590/S1516-35982011001000010 http://hdl.handle.net/11449/2599 |
identifier_str_mv |
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, 2011. 1516-3598 10.1590/S1516-35982011001000010 S1516-35982011001000010 WOS:000297341400010 S1516-35982011001000010.pdf 2251116139872527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Zootecnia 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2135-2141 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129429362180096 |