Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjm.2015.07.001 http://hdl.handle.net/11449/168453 |
Resumo: | This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after infection with a highly pathogenic Newcastle disease virus strain (São Joao do Meriti) and that, in chickens, prevent clinical signs. Infected pigeons shed the pathogenic strain, which was not detected in sentinel chickens or control birds. When the presence of Newcastle disease virus was analyzed in tissue samples by RT-PCR, in both species, the virus was most frequently found in the spleen. The vaccination regimen can prevent clinical disease in chickens and reduce viral shedding by chickens or pigeons. Biosecurity measures associated with vaccination programs are crucial to maintain a virulent Newcastle disease virus-free status in industrial poultry in Brazil. |
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Repositório Institucional da UNESP |
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Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickensColumba liviaExperimental infectionGallus gallusRT-PCRSerologyThis study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after infection with a highly pathogenic Newcastle disease virus strain (São Joao do Meriti) and that, in chickens, prevent clinical signs. Infected pigeons shed the pathogenic strain, which was not detected in sentinel chickens or control birds. When the presence of Newcastle disease virus was analyzed in tissue samples by RT-PCR, in both species, the virus was most frequently found in the spleen. The vaccination regimen can prevent clinical disease in chickens and reduce viral shedding by chickens or pigeons. Biosecurity measures associated with vaccination programs are crucial to maintain a virulent Newcastle disease virus-free status in industrial poultry in Brazil.Departamento de Medicina Veterinária Universidade Estadual do Centro-Oeste (UNICENTRO)Departamento de Patologia Veterinária Universidade Estadual Paulista (UNESP)Departamento de Patologia Veterinária Universidade Estadual Paulista (UNESP)Universidade Estadual do Centro-Oeste (UNICENTRO)Universidade Estadual Paulista (Unesp)Carrascoa, Adriano de Oliveira TorresSekia, Meire ChristinaBenevenutea, Jyan LucasIkeda, PriscilaPinto, Aramis Augusto [UNESP]2018-12-11T16:41:20Z2018-12-11T16:41:20Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article231-242application/pdfhttp://dx.doi.org/10.1016/j.bjm.2015.07.001Brazilian Journal of Microbiology, v. 47, n. 1, p. 231-242, 2016.1678-44051517-8382http://hdl.handle.net/11449/16845310.1016/j.bjm.2015.07.001S1517-838220160001002312-s2.0-84960099352S1517-83822016000100231.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology0,630info:eu-repo/semantics/openAccess2024-01-09T06:31:00Zoai:repositorio.unesp.br:11449/168453Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-09T06:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
title |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
spellingShingle |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens Carrascoa, Adriano de Oliveira Torres Columba livia Experimental infection Gallus gallus RT-PCR Serology |
title_short |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
title_full |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
title_fullStr |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
title_full_unstemmed |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
title_sort |
Experimental infection with Brazilian newcastle disease virus strain in pigeons and chickens |
author |
Carrascoa, Adriano de Oliveira Torres |
author_facet |
Carrascoa, Adriano de Oliveira Torres Sekia, Meire Christina Benevenutea, Jyan Lucas Ikeda, Priscila Pinto, Aramis Augusto [UNESP] |
author_role |
author |
author2 |
Sekia, Meire Christina Benevenutea, Jyan Lucas Ikeda, Priscila Pinto, Aramis Augusto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual do Centro-Oeste (UNICENTRO) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carrascoa, Adriano de Oliveira Torres Sekia, Meire Christina Benevenutea, Jyan Lucas Ikeda, Priscila Pinto, Aramis Augusto [UNESP] |
dc.subject.por.fl_str_mv |
Columba livia Experimental infection Gallus gallus RT-PCR Serology |
topic |
Columba livia Experimental infection Gallus gallus RT-PCR Serology |
description |
This study was designed with the goal of adding as much information as possible about the role of pigeons (Columba livia) and chickens (Gallus gallus) in Newcastle disease virus epidemiology. These species were submitted to direct experimental infection with Newcastle disease virus to evaluate interspecies transmission and virus-host relationships. The results obtained in four experimental models were analyzed by hemagglutination inhibition and reverse transcriptase polymerase chain reaction for detection of virus shedding. These techniques revealed that both avian species, when previously immunized with a low pathogenic Newcastle disease virus strain (LaSota), developed high antibody titers that significantly reduced virus shedding after infection with a highly pathogenic Newcastle disease virus strain (São Joao do Meriti) and that, in chickens, prevent clinical signs. Infected pigeons shed the pathogenic strain, which was not detected in sentinel chickens or control birds. When the presence of Newcastle disease virus was analyzed in tissue samples by RT-PCR, in both species, the virus was most frequently found in the spleen. The vaccination regimen can prevent clinical disease in chickens and reduce viral shedding by chickens or pigeons. Biosecurity measures associated with vaccination programs are crucial to maintain a virulent Newcastle disease virus-free status in industrial poultry in Brazil. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T16:41:20Z 2018-12-11T16:41:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjm.2015.07.001 Brazilian Journal of Microbiology, v. 47, n. 1, p. 231-242, 2016. 1678-4405 1517-8382 http://hdl.handle.net/11449/168453 10.1016/j.bjm.2015.07.001 S1517-83822016000100231 2-s2.0-84960099352 S1517-83822016000100231.pdf |
url |
http://dx.doi.org/10.1016/j.bjm.2015.07.001 http://hdl.handle.net/11449/168453 |
identifier_str_mv |
Brazilian Journal of Microbiology, v. 47, n. 1, p. 231-242, 2016. 1678-4405 1517-8382 10.1016/j.bjm.2015.07.001 S1517-83822016000100231 2-s2.0-84960099352 S1517-83822016000100231.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
231-242 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965572004315136 |