Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572009000500017 http://hdl.handle.net/11449/225685 |
Resumo: | Aggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated. |
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Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, BrazilBacteriaGingivitisNative AmericansPeriodontitisAggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated.Department of Pathology and Propedeutics Araçatuba Dental School São Paulo State University, Araçatuba, SPDepartment of Dentistry University of Cuiabá, Cuiabá, MTDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo, São Paulo, SPCenter of Mathematics Computation and Cognition Federal University of ABC, Santo André, SPDepartment of Pathology and Propedeutics Araçatuba Dental School São Paulo State University, Araçatuba, SPUniversidade Estadual Paulista (UNESP)University of CuiabáUniversidade de São Paulo (USP)Federal University of ABCVieira, Evanice Menezes Marçal [UNESP]Raslan, Suzane A.Wahasugui, Thais CristinaAvila-Campos, Mario JulioMarvulle, ValdecirGaetti-Jardim Júnior, Elerson [UNESP]2022-04-28T20:58:06Z2022-04-28T20:58:06Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article440-445http://dx.doi.org/10.1590/S1678-77572009000500017Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009.1678-77651678-7757http://hdl.handle.net/11449/22568510.1590/S1678-775720090005000172-s2.0-70749095323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-09-19T18:57:13Zoai:repositorio.unesp.br:11449/225685Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
title |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
spellingShingle |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil Vieira, Evanice Menezes Marçal [UNESP] Bacteria Gingivitis Native Americans Periodontitis |
title_short |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
title_full |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
title_fullStr |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
title_full_unstemmed |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
title_sort |
Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil |
author |
Vieira, Evanice Menezes Marçal [UNESP] |
author_facet |
Vieira, Evanice Menezes Marçal [UNESP] Raslan, Suzane A. Wahasugui, Thais Cristina Avila-Campos, Mario Julio Marvulle, Valdecir Gaetti-Jardim Júnior, Elerson [UNESP] |
author_role |
author |
author2 |
Raslan, Suzane A. Wahasugui, Thais Cristina Avila-Campos, Mario Julio Marvulle, Valdecir Gaetti-Jardim Júnior, Elerson [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Cuiabá Universidade de São Paulo (USP) Federal University of ABC |
dc.contributor.author.fl_str_mv |
Vieira, Evanice Menezes Marçal [UNESP] Raslan, Suzane A. Wahasugui, Thais Cristina Avila-Campos, Mario Julio Marvulle, Valdecir Gaetti-Jardim Júnior, Elerson [UNESP] |
dc.subject.por.fl_str_mv |
Bacteria Gingivitis Native Americans Periodontitis |
topic |
Bacteria Gingivitis Native Americans Periodontitis |
description |
Aggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:58:06Z 2022-04-28T20:58:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572009000500017 Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009. 1678-7765 1678-7757 http://hdl.handle.net/11449/225685 10.1590/S1678-77572009000500017 2-s2.0-70749095323 |
url |
http://dx.doi.org/10.1590/S1678-77572009000500017 http://hdl.handle.net/11449/225685 |
identifier_str_mv |
Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009. 1678-7765 1678-7757 10.1590/S1678-77572009000500017 2-s2.0-70749095323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
440-445 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546397047193600 |