Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil

Detalhes bibliográficos
Autor(a) principal: Vieira, Evanice Menezes Marçal [UNESP]
Data de Publicação: 2009
Outros Autores: Raslan, Suzane A., Wahasugui, Thais Cristina, Avila-Campos, Mario Julio, Marvulle, Valdecir, Gaetti-Jardim Júnior, Elerson [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-77572009000500017
http://hdl.handle.net/11449/225685
Resumo: Aggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated.
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spelling Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, BrazilBacteriaGingivitisNative AmericansPeriodontitisAggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated.Department of Pathology and Propedeutics Araçatuba Dental School São Paulo State University, Araçatuba, SPDepartment of Dentistry University of Cuiabá, Cuiabá, MTDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo, São Paulo, SPCenter of Mathematics Computation and Cognition Federal University of ABC, Santo André, SPDepartment of Pathology and Propedeutics Araçatuba Dental School São Paulo State University, Araçatuba, SPUniversidade Estadual Paulista (UNESP)University of CuiabáUniversidade de São Paulo (USP)Federal University of ABCVieira, Evanice Menezes Marçal [UNESP]Raslan, Suzane A.Wahasugui, Thais CristinaAvila-Campos, Mario JulioMarvulle, ValdecirGaetti-Jardim Júnior, Elerson [UNESP]2022-04-28T20:58:06Z2022-04-28T20:58:06Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article440-445http://dx.doi.org/10.1590/S1678-77572009000500017Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009.1678-77651678-7757http://hdl.handle.net/11449/22568510.1590/S1678-775720090005000172-s2.0-70749095323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2024-09-19T18:57:13Zoai:repositorio.unesp.br:11449/225685Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
title Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
spellingShingle Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
Vieira, Evanice Menezes Marçal [UNESP]
Bacteria
Gingivitis
Native Americans
Periodontitis
title_short Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
title_full Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
title_fullStr Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
title_full_unstemmed Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
title_sort Occurrence of Aggregatibacter actinomycetemcomitans in Brazilian Indians from umutina reservation, Mato Grosso, Brazil
author Vieira, Evanice Menezes Marçal [UNESP]
author_facet Vieira, Evanice Menezes Marçal [UNESP]
Raslan, Suzane A.
Wahasugui, Thais Cristina
Avila-Campos, Mario Julio
Marvulle, Valdecir
Gaetti-Jardim Júnior, Elerson [UNESP]
author_role author
author2 Raslan, Suzane A.
Wahasugui, Thais Cristina
Avila-Campos, Mario Julio
Marvulle, Valdecir
Gaetti-Jardim Júnior, Elerson [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Cuiabá
Universidade de São Paulo (USP)
Federal University of ABC
dc.contributor.author.fl_str_mv Vieira, Evanice Menezes Marçal [UNESP]
Raslan, Suzane A.
Wahasugui, Thais Cristina
Avila-Campos, Mario Julio
Marvulle, Valdecir
Gaetti-Jardim Júnior, Elerson [UNESP]
dc.subject.por.fl_str_mv Bacteria
Gingivitis
Native Americans
Periodontitis
topic Bacteria
Gingivitis
Native Americans
Periodontitis
description Aggregatibacter actinomycetemcomitans is associated with periodontal disease, especially localized aggressive periodontitis, produces a potent leukotoxin and its distribution is influenced by ethnic characteristics of the population. Objective: Using culture and polymerase chain reaction (PCR) techniques, this study evaluated the occurrence of this microorganism and the distribution of leukotoxic strains isolated from Indians belonging to the Umutima Reservation, Mato Grosso, Brazil. Material and Methods: Forty- eight native Brazilians with gingivitis and 38 with chronic periodontitis, belonging to Umutina, Paresi, Bororo, Bakairi, Kayabi, Irantxe, Nambikwara and Terena ethnicities, were studied. Subgingival, supragingival and saliva samples of each patient were collected and transferred to VMGA III medium and to ultra pure Milli Q water. Bacteria were grown on TSBV agar and incubated in anaerobiosis (90% N2 + 10% CO2) at 37°C for 72 h. The presence of the ltx promoter was determined by PCR, and a 530 bp deletion in the promoter was evaluated by using specific primers. Results: A. actinomycetemcomitans was isolated from 8.33% of saliva, supragingival and subgingival samples from patients with gingivitis and from 18.42% of saliva and supragingival biofilm, and 26.32% subgingival biofilm from patients with chronic periodontitis. By PCR, the bacterial DNA was detected in 8.33% of saliva, supragingival and subgingival biofilms from patients with gingivitis and from 23.68% of saliva, 28.95% supragingival biofilm and 34.21% subgingival biofilm from patients with periodontitis. All strains were grouped as non-JP2 clones based on the absence of deletion in the leukotoxin promoter. Differences among the microbial and clinical parameters in patients were analyzed by using the Mann-Whitney, Chi-square or Fisher's exact tests. Conclusions: The present results suggest that A. actinomycetemcomitans can be related to the attachment loss in this population, but the presence of minimally leukotoxic strains, as well as its role in the pathogenesis of the periodontitis in these native Brazilians need to be further investigated.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
2022-04-28T20:58:06Z
2022-04-28T20:58:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572009000500017
Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009.
1678-7765
1678-7757
http://hdl.handle.net/11449/225685
10.1590/S1678-77572009000500017
2-s2.0-70749095323
url http://dx.doi.org/10.1590/S1678-77572009000500017
http://hdl.handle.net/11449/225685
identifier_str_mv Journal of Applied Oral Science, v. 17, n. 5, p. 440-445, 2009.
1678-7765
1678-7757
10.1590/S1678-77572009000500017
2-s2.0-70749095323
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 440-445
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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