Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas

Detalhes bibliográficos
Autor(a) principal: De Brito, Maila K. Mattos [UNESP]
Data de Publicação: 2006
Outros Autores: Camargo Filho, José Carlos Silva [UNESP], Vanderlei, Luiz Carlos Marques [UNESP], Tarumoto, Mário Hissamitsu [UNESP], Dal Pai, Vitalino, Giacometti, José A. [UNESP]
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/231806
Resumo: The purpose of this paper was to assess a new methodology to analyze digitalized cross sectional images from the skeletal muscular fibers in rats submitted to physical exercise on treadmill. It was used portions of the soleum muscle of rats attained from histological cuts and tinted with hematoxylin and eosin (HE). 100 muscular fibers were assessed from each animal, and their perimeter, the area, and the maximal, medium and minimal diameters were measured by means of the segmentation process from digitalized images of the fibers' sections using the software Image-Pro Plus. The geometrical dimensions such as the area, the perimeter and the medium and minimal diameters of the cross section of muscular fibers revealed to be adequate to analyze the effect of the training in rats. The analysis revealed the existence of an interaction between the group of rats and the duration of the physical exercise. The Pearson's correlation coefficient was higher between the medium diameter and the area of the fibers (0.97), followed by the correlation between the maximal and medium diameter with the perimeter (0.93). It can be concluded that the measurement of the grade of the hypertrophy of the muscular fibers can be performed by determining the medium diameter or the cross sectional area of the fiber, and thus constituting an adequate and effective methodology especially for muscular fibers with accentuated polymorphism.
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spelling Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadasGeographical dimensions of fibers from the soleum muscle in rats exercised on treadmill: The importance of the analysis by means of digitalized imagesMuscular fibersPhysical training with animalsVideo microscopyThe purpose of this paper was to assess a new methodology to analyze digitalized cross sectional images from the skeletal muscular fibers in rats submitted to physical exercise on treadmill. It was used portions of the soleum muscle of rats attained from histological cuts and tinted with hematoxylin and eosin (HE). 100 muscular fibers were assessed from each animal, and their perimeter, the area, and the maximal, medium and minimal diameters were measured by means of the segmentation process from digitalized images of the fibers' sections using the software Image-Pro Plus. The geometrical dimensions such as the area, the perimeter and the medium and minimal diameters of the cross section of muscular fibers revealed to be adequate to analyze the effect of the training in rats. The analysis revealed the existence of an interaction between the group of rats and the duration of the physical exercise. The Pearson's correlation coefficient was higher between the medium diameter and the area of the fibers (0.97), followed by the correlation between the maximal and medium diameter with the perimeter (0.93). It can be concluded that the measurement of the grade of the hypertrophy of the muscular fibers can be performed by determining the medium diameter or the cross sectional area of the fiber, and thus constituting an adequate and effective methodology especially for muscular fibers with accentuated polymorphism.College of Sciences and Technology Unesp, P.O. Box 467, 19060-900 - Presidente Prudente, SPOeste Paulista University UNIOESTE, Rua José Bongiovani, 700, 19050-900 - Presidente Prudente, SPCollege of Sciences and Technology Unesp, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPCollege of Sciences and Technology Unesp, P.O. Box 467, 19060-900 - Presidente Prudente, SPCollege of Sciences and Technology Unesp, Rua Roberto Simonsen, 305, 19060-900 - Presidente Prudente, SPUniversidade Estadual Paulista (UNESP)UNIOESTEDe Brito, Maila K. Mattos [UNESP]Camargo Filho, José Carlos Silva [UNESP]Vanderlei, Luiz Carlos Marques [UNESP]Tarumoto, Mário Hissamitsu [UNESP]Dal Pai, VitalinoGiacometti, José A. [UNESP]2022-04-29T08:47:39Z2022-04-29T08:47:39Z2006-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Medicina do Esporte, v. 12, n. 2, 2006.1517-8692http://hdl.handle.net/11449/2318062-s2.0-34248328799Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporengRevista Brasileira de Medicina do Esporteinfo:eu-repo/semantics/openAccess2024-06-19T12:44:01Zoai:repositorio.unesp.br:11449/231806Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:12:46.789418Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
Geographical dimensions of fibers from the soleum muscle in rats exercised on treadmill: The importance of the analysis by means of digitalized images
title Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
spellingShingle Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
De Brito, Maila K. Mattos [UNESP]
Muscular fibers
Physical training with animals
Video microscopy
title_short Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
title_full Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
title_fullStr Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
title_full_unstemmed Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
title_sort Dimensões geométricas das fibras do músculo sóleo de ratos exercitados em esteira rolante: A importância da análise por meio de imagens digitalizadas
author De Brito, Maila K. Mattos [UNESP]
author_facet De Brito, Maila K. Mattos [UNESP]
Camargo Filho, José Carlos Silva [UNESP]
Vanderlei, Luiz Carlos Marques [UNESP]
Tarumoto, Mário Hissamitsu [UNESP]
Dal Pai, Vitalino
Giacometti, José A. [UNESP]
author_role author
author2 Camargo Filho, José Carlos Silva [UNESP]
Vanderlei, Luiz Carlos Marques [UNESP]
Tarumoto, Mário Hissamitsu [UNESP]
Dal Pai, Vitalino
Giacometti, José A. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
UNIOESTE
dc.contributor.author.fl_str_mv De Brito, Maila K. Mattos [UNESP]
Camargo Filho, José Carlos Silva [UNESP]
Vanderlei, Luiz Carlos Marques [UNESP]
Tarumoto, Mário Hissamitsu [UNESP]
Dal Pai, Vitalino
Giacometti, José A. [UNESP]
dc.subject.por.fl_str_mv Muscular fibers
Physical training with animals
Video microscopy
topic Muscular fibers
Physical training with animals
Video microscopy
description The purpose of this paper was to assess a new methodology to analyze digitalized cross sectional images from the skeletal muscular fibers in rats submitted to physical exercise on treadmill. It was used portions of the soleum muscle of rats attained from histological cuts and tinted with hematoxylin and eosin (HE). 100 muscular fibers were assessed from each animal, and their perimeter, the area, and the maximal, medium and minimal diameters were measured by means of the segmentation process from digitalized images of the fibers' sections using the software Image-Pro Plus. The geometrical dimensions such as the area, the perimeter and the medium and minimal diameters of the cross section of muscular fibers revealed to be adequate to analyze the effect of the training in rats. The analysis revealed the existence of an interaction between the group of rats and the duration of the physical exercise. The Pearson's correlation coefficient was higher between the medium diameter and the area of the fibers (0.97), followed by the correlation between the maximal and medium diameter with the perimeter (0.93). It can be concluded that the measurement of the grade of the hypertrophy of the muscular fibers can be performed by determining the medium diameter or the cross sectional area of the fiber, and thus constituting an adequate and effective methodology especially for muscular fibers with accentuated polymorphism.
publishDate 2006
dc.date.none.fl_str_mv 2006-03-01
2022-04-29T08:47:39Z
2022-04-29T08:47:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Medicina do Esporte, v. 12, n. 2, 2006.
1517-8692
http://hdl.handle.net/11449/231806
2-s2.0-34248328799
identifier_str_mv Revista Brasileira de Medicina do Esporte, v. 12, n. 2, 2006.
1517-8692
2-s2.0-34248328799
url http://hdl.handle.net/11449/231806
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv Revista Brasileira de Medicina do Esporte
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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