The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis

Detalhes bibliográficos
Autor(a) principal: Bezerra-Souza, Adriana
Data de Publicação: 2016
Outros Autores: Yamamoto, Eduardo S., Laurenti, Márcia D., Ribeiro, Susan P., Passero, Luiz Felipe D. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.parint.2016.08.003
http://hdl.handle.net/11449/173544
Resumo: The production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively.
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spelling The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisAntileishmanial agentButenafineDrug repurposingLeishmania (Leishmania) amazonensisLeishmania (Viannia) braziliensisThe production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Pathology of Infectious Diseases (LIM-50) Medical School University of São Paulo, Avenida Dr. Arnaldo 455Case Western Reserve University Pathology DepartmentDivision of Clinical Immunology and Allergy LIM60 University of Sao Paulo School of MedicineSão Vicente Unit Paulista Coastal Campus Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Praça Infante Dom Henrique, s/nSão Vicente Unit Paulista Coastal Campus Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Praça Infante Dom Henrique, s/nFAPESP: 2015/18746-4Universidade de São Paulo (USP)Pathology DepartmentUniversidade Estadual Paulista (Unesp)Bezerra-Souza, AdrianaYamamoto, Eduardo S.Laurenti, Márcia D.Ribeiro, Susan P.Passero, Luiz Felipe D. [UNESP]2018-12-11T17:06:11Z2018-12-11T17:06:11Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article702-707application/pdfhttp://dx.doi.org/10.1016/j.parint.2016.08.003Parasitology International, v. 65, n. 6, p. 702-707, 2016.1873-03291383-5769http://hdl.handle.net/11449/17354410.1016/j.parint.2016.08.0032-s2.0-849892450482-s2.0-84989245048.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology International0,914info:eu-repo/semantics/openAccess2024-01-09T06:29:24Zoai:repositorio.unesp.br:11449/173544Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:05.959Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
title The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
spellingShingle The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
Bezerra-Souza, Adriana
Antileishmanial agent
Butenafine
Drug repurposing
Leishmania (Leishmania) amazonensis
Leishmania (Viannia) braziliensis
title_short The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
title_full The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
title_fullStr The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
title_full_unstemmed The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
title_sort The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
author Bezerra-Souza, Adriana
author_facet Bezerra-Souza, Adriana
Yamamoto, Eduardo S.
Laurenti, Márcia D.
Ribeiro, Susan P.
Passero, Luiz Felipe D. [UNESP]
author_role author
author2 Yamamoto, Eduardo S.
Laurenti, Márcia D.
Ribeiro, Susan P.
Passero, Luiz Felipe D. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Pathology Department
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Bezerra-Souza, Adriana
Yamamoto, Eduardo S.
Laurenti, Márcia D.
Ribeiro, Susan P.
Passero, Luiz Felipe D. [UNESP]
dc.subject.por.fl_str_mv Antileishmanial agent
Butenafine
Drug repurposing
Leishmania (Leishmania) amazonensis
Leishmania (Viannia) braziliensis
topic Antileishmanial agent
Butenafine
Drug repurposing
Leishmania (Leishmania) amazonensis
Leishmania (Viannia) braziliensis
description The production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
2018-12-11T17:06:11Z
2018-12-11T17:06:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.parint.2016.08.003
Parasitology International, v. 65, n. 6, p. 702-707, 2016.
1873-0329
1383-5769
http://hdl.handle.net/11449/173544
10.1016/j.parint.2016.08.003
2-s2.0-84989245048
2-s2.0-84989245048.pdf
url http://dx.doi.org/10.1016/j.parint.2016.08.003
http://hdl.handle.net/11449/173544
identifier_str_mv Parasitology International, v. 65, n. 6, p. 702-707, 2016.
1873-0329
1383-5769
10.1016/j.parint.2016.08.003
2-s2.0-84989245048
2-s2.0-84989245048.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology International
0,914
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 702-707
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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