The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.parint.2016.08.003 http://hdl.handle.net/11449/173544 |
Resumo: | The production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively. |
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The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensisAntileishmanial agentButenafineDrug repurposingLeishmania (Leishmania) amazonensisLeishmania (Viannia) braziliensisThe production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Pathology of Infectious Diseases (LIM-50) Medical School University of São Paulo, Avenida Dr. Arnaldo 455Case Western Reserve University Pathology DepartmentDivision of Clinical Immunology and Allergy LIM60 University of Sao Paulo School of MedicineSão Vicente Unit Paulista Coastal Campus Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Praça Infante Dom Henrique, s/nSão Vicente Unit Paulista Coastal Campus Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Praça Infante Dom Henrique, s/nFAPESP: 2015/18746-4Universidade de São Paulo (USP)Pathology DepartmentUniversidade Estadual Paulista (Unesp)Bezerra-Souza, AdrianaYamamoto, Eduardo S.Laurenti, Márcia D.Ribeiro, Susan P.Passero, Luiz Felipe D. [UNESP]2018-12-11T17:06:11Z2018-12-11T17:06:11Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article702-707application/pdfhttp://dx.doi.org/10.1016/j.parint.2016.08.003Parasitology International, v. 65, n. 6, p. 702-707, 2016.1873-03291383-5769http://hdl.handle.net/11449/17354410.1016/j.parint.2016.08.0032-s2.0-849892450482-s2.0-84989245048.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology International0,914info:eu-repo/semantics/openAccess2024-01-09T06:29:24Zoai:repositorio.unesp.br:11449/173544Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:05.959Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
title |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
spellingShingle |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis Bezerra-Souza, Adriana Antileishmanial agent Butenafine Drug repurposing Leishmania (Leishmania) amazonensis Leishmania (Viannia) braziliensis |
title_short |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
title_full |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
title_fullStr |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
title_full_unstemmed |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
title_sort |
The antifungal compound butenafine eliminates promastigote and amastigote forms of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis |
author |
Bezerra-Souza, Adriana |
author_facet |
Bezerra-Souza, Adriana Yamamoto, Eduardo S. Laurenti, Márcia D. Ribeiro, Susan P. Passero, Luiz Felipe D. [UNESP] |
author_role |
author |
author2 |
Yamamoto, Eduardo S. Laurenti, Márcia D. Ribeiro, Susan P. Passero, Luiz Felipe D. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Pathology Department Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bezerra-Souza, Adriana Yamamoto, Eduardo S. Laurenti, Márcia D. Ribeiro, Susan P. Passero, Luiz Felipe D. [UNESP] |
dc.subject.por.fl_str_mv |
Antileishmanial agent Butenafine Drug repurposing Leishmania (Leishmania) amazonensis Leishmania (Viannia) braziliensis |
topic |
Antileishmanial agent Butenafine Drug repurposing Leishmania (Leishmania) amazonensis Leishmania (Viannia) braziliensis |
description |
The production of ergosterol lipid, important for the Leishmania membrane homeostasis, involves different enzymes. This pathway can be blocked to azoles and allylamines drugs, such as Butenafine. The aim of the present work was to evaluate the anti-leishmanicidal activity of this drug in 2 major species of Leishmania responsible for causing the American tegumentar leishmaniasis (L. (L.) amazonensis and L. (V.) braziliensis). Butenafine eliminated promastigote forms of L. amazonensis and L. braziliensis with efficacy similar to miltefosine, a standard anti-leishmania drug. In addition, butenafine induced alterations in promastigote forms of L. amazonensis that resemble programmed cell death. Butenafine as well as miltefosine presented mild toxicity in peritoneal macrophages, however, butenafine was more effective to eliminate intracellular amastigotes of both L. amazonensis and L. braziliensis, and this effect was not associated with elevated levels of nitric oxide or hydrogen peroxide. Taken together, data presented herein suggests that butenafine can be considered as a prototype drug able to eliminate L. amazonensis and L. braziliensis, etiological agents of anergic diffuse and mucocutaneous leishmaniasis, respectively. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 2018-12-11T17:06:11Z 2018-12-11T17:06:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.parint.2016.08.003 Parasitology International, v. 65, n. 6, p. 702-707, 2016. 1873-0329 1383-5769 http://hdl.handle.net/11449/173544 10.1016/j.parint.2016.08.003 2-s2.0-84989245048 2-s2.0-84989245048.pdf |
url |
http://dx.doi.org/10.1016/j.parint.2016.08.003 http://hdl.handle.net/11449/173544 |
identifier_str_mv |
Parasitology International, v. 65, n. 6, p. 702-707, 2016. 1873-0329 1383-5769 10.1016/j.parint.2016.08.003 2-s2.0-84989245048 2-s2.0-84989245048.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology International 0,914 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
702-707 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129436852158464 |