Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40659-020-00298-z http://hdl.handle.net/11449/199089 |
Resumo: | Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H O ) and expected (H E ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy. |
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Repositório Institucional da UNESP |
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Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related speciesEremanthus erythropappusEremanthus glomerulatusEremanthus incanusGenetic diversitySimple sequence repeatsBackground: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H O ) and expected (H E ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.Department of Forest Science São Paulo State University UNESPDepartment of Forest Sciences Laboratory of Tree Genetic Conservation Federal University of Lavras UFLADepartment of Biology Federal University of Minas Gerais UFMGDepartment of Forest Science São Paulo State University UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal de Lavras (UFLA)Universidade Federal de Minas Gerais (UFMG)Rocha, Lucas Fernandes [UNESP]Paula, Natália RibeiroNazareno, Alison GonçalvesDe Carvalho, Dulcinéia2020-12-12T01:30:30Z2020-12-12T01:30:30Z2020-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s40659-020-00298-zBiological Research, v. 53, n. 1, 2020.0717-62870716-9760http://hdl.handle.net/11449/19908910.1186/s40659-020-00298-z2-s2.0-85087738795Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Researchinfo:eu-repo/semantics/openAccess2021-10-23T03:03:36Zoai:repositorio.unesp.br:11449/199089Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T03:03:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
title |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
spellingShingle |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species Rocha, Lucas Fernandes [UNESP] Eremanthus erythropappus Eremanthus glomerulatus Eremanthus incanus Genetic diversity Simple sequence repeats |
title_short |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
title_full |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
title_fullStr |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
title_full_unstemmed |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
title_sort |
Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species |
author |
Rocha, Lucas Fernandes [UNESP] |
author_facet |
Rocha, Lucas Fernandes [UNESP] Paula, Natália Ribeiro Nazareno, Alison Gonçalves De Carvalho, Dulcinéia |
author_role |
author |
author2 |
Paula, Natália Ribeiro Nazareno, Alison Gonçalves De Carvalho, Dulcinéia |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Lavras (UFLA) Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Rocha, Lucas Fernandes [UNESP] Paula, Natália Ribeiro Nazareno, Alison Gonçalves De Carvalho, Dulcinéia |
dc.subject.por.fl_str_mv |
Eremanthus erythropappus Eremanthus glomerulatus Eremanthus incanus Genetic diversity Simple sequence repeats |
topic |
Eremanthus erythropappus Eremanthus glomerulatus Eremanthus incanus Genetic diversity Simple sequence repeats |
description |
Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed (H O ) and expected (H E ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:30:30Z 2020-12-12T01:30:30Z 2020-07-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40659-020-00298-z Biological Research, v. 53, n. 1, 2020. 0717-6287 0716-9760 http://hdl.handle.net/11449/199089 10.1186/s40659-020-00298-z 2-s2.0-85087738795 |
url |
http://dx.doi.org/10.1186/s40659-020-00298-z http://hdl.handle.net/11449/199089 |
identifier_str_mv |
Biological Research, v. 53, n. 1, 2020. 0717-6287 0716-9760 10.1186/s40659-020-00298-z 2-s2.0-85087738795 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046648282087424 |