First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella

Detalhes bibliográficos
Autor(a) principal: do Amaral, Renan Bressianini [UNESP]
Data de Publicação: 2022
Outros Autores: Cardozo, Marita Vedovelli [UNESP], Varani, Alessandro de Mello [UNESP], Furquim, Maria Eduarda Chiaradia [UNESP], Dias, Clara Morato [UNESP], Assis, William Oliveira de, da Silva, Alanderson Rodrigues, Herrera, Heitor Miraglia, Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/microorganisms10081609
http://hdl.handle.net/11449/241617
Resumo: The genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.
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spelling First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonellabartonelosisbiochemical characterizationmarsupialiaphylogenomicstaxonomic classificationWGSThe genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.Programa de Pós-Graduação em Microbiologia Agropecuária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPLaboratório de Imunoparasitologia Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPDepartamento de Biotecnologia Agropecuária e Ambiental Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPLaboratório de Biologia Parasitária Universidade Católica Dom Bosco, MSPrograma de Pós-Graduação em Microbiologia Agropecuária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPLaboratório de Imunoparasitologia Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPDepartamento de Biotecnologia Agropecuária e Ambiental Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (UNESP), SPUniversidade Estadual Paulista (UNESP)Universidade Católica Dom Boscodo Amaral, Renan Bressianini [UNESP]Cardozo, Marita Vedovelli [UNESP]Varani, Alessandro de Mello [UNESP]Furquim, Maria Eduarda Chiaradia [UNESP]Dias, Clara Morato [UNESP]Assis, William Oliveira deda Silva, Alanderson RodriguesHerrera, Heitor MiragliaMachado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2023-03-01T21:13:11Z2023-03-01T21:13:11Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms10081609Microorganisms, v. 10, n. 8, 2022.2076-2607http://hdl.handle.net/11449/24161710.3390/microorganisms100816092-s2.0-85137368199Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2024-06-07T15:32:23Zoai:repositorio.unesp.br:11449/241617Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:44.067492Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
title First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
spellingShingle First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
do Amaral, Renan Bressianini [UNESP]
bartonelosis
biochemical characterization
marsupialia
phylogenomics
taxonomic classification
WGS
title_short First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
title_full First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
title_fullStr First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
title_full_unstemmed First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
title_sort First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella
author do Amaral, Renan Bressianini [UNESP]
author_facet do Amaral, Renan Bressianini [UNESP]
Cardozo, Marita Vedovelli [UNESP]
Varani, Alessandro de Mello [UNESP]
Furquim, Maria Eduarda Chiaradia [UNESP]
Dias, Clara Morato [UNESP]
Assis, William Oliveira de
da Silva, Alanderson Rodrigues
Herrera, Heitor Miraglia
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 Cardozo, Marita Vedovelli [UNESP]
Varani, Alessandro de Mello [UNESP]
Furquim, Maria Eduarda Chiaradia [UNESP]
Dias, Clara Morato [UNESP]
Assis, William Oliveira de
da Silva, Alanderson Rodrigues
Herrera, Heitor Miraglia
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Católica Dom Bosco
dc.contributor.author.fl_str_mv do Amaral, Renan Bressianini [UNESP]
Cardozo, Marita Vedovelli [UNESP]
Varani, Alessandro de Mello [UNESP]
Furquim, Maria Eduarda Chiaradia [UNESP]
Dias, Clara Morato [UNESP]
Assis, William Oliveira de
da Silva, Alanderson Rodrigues
Herrera, Heitor Miraglia
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv bartonelosis
biochemical characterization
marsupialia
phylogenomics
taxonomic classification
WGS
topic bartonelosis
biochemical characterization
marsupialia
phylogenomics
taxonomic classification
WGS
description The genus Bartonella (Rhizobiales: Bartonellaceae) encompasses facultative intracellular Gram-negative alphaproteobacteria that parasitize mainly erythrocytes and endothelial cells, as well as macrophages, monocytes and dendritic cells. Although they can infect numerous mammal species and arthropod vectors worldwide, reports of Bartonella infections in marsupials are scarce. In fact, such agents have only been detected in marsupials and/or associated ectoparasites in Australia and the United States of America until the present moment. The present study aimed to isolate and characterize molecularly, morphologically and phenotypically Bartonella infecting free-living marsupials sampled in the Brazilian Pantanal, the largest wetland in South America. Two marsupials were captured in December 2018 and six marsupials in February 2019, totaling eight small mammals sampled: five (62.5%) Thylamys macrurus and three (37.5%) Monodelphis domestica. All blood samples were submitted to qPCR for Bartonella spp. based on the nuoG gene, a pre-enrichment liquid culture and a chocolate agar solid culture. Bartonella sp. was isolated from 3 T. macrurus and one M. domestica. One Bartonella isolate obtained from a T. macrurus blood sample (strain 117A) that showed to be closely related to the Bartonella vinsonii complex and Bartonella machadoae was selected for whole genome sequencing using a hybrid approach based on Illumina NovaSeq and Nanopore sequencing platforms. This strain showed a genome of 2.35 Mbp, with an average C + G content of 38.8%, coding for 2013 genes, and a 29 kb plasmid with an average C + G content of 34.5%. In addition, this strain exhibited an average nucleotide identity (ANI) of 85% with Bartonella species belonging to the B. vinsonii group and 91% with B. machadoae. Phylogenomic analysis based on 291 protein coding genes shared by the genomes of 53 Bartonella species positioned this strain closely to B. machadoae. This new isolated species was named Bartonella harrusi sp. nov., which was characterized as having small capnophilic, microaerophilic and aerobic rods with an absence of pili and flagella. In conclusion, the present work describes the biochemical, phenotypic and genomic characteristics of Bartonella harrusi, a new species isolated from the T. macrurus blood samples of the Brazilian Pantanal. Finally, a review of the taxonomic classification of members of the genus Bartonella is proposed, based on the ANI values accessed by whole genome sequencing analyses.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-01
2023-03-01T21:13:11Z
2023-03-01T21:13:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/microorganisms10081609
Microorganisms, v. 10, n. 8, 2022.
2076-2607
http://hdl.handle.net/11449/241617
10.3390/microorganisms10081609
2-s2.0-85137368199
url http://dx.doi.org/10.3390/microorganisms10081609
http://hdl.handle.net/11449/241617
identifier_str_mv Microorganisms, v. 10, n. 8, 2022.
2076-2607
10.3390/microorganisms10081609
2-s2.0-85137368199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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