Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods

Detalhes bibliográficos
Autor(a) principal: Gomes, Brenda P. F. A.
Data de Publicação: 2021
Outros Autores: Bronzato, Juliana D., Almeida-Gomes, Rebecca F., Pinheiro, Ericka T., Sousa, Ezilmara L. R., Jacinto, Rogério C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-021-03923-7
http://hdl.handle.net/11449/207620
Resumo: Objective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.
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spelling Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methodsCultureEndodonticsFusobacterium nucleatumPCRObjective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.Department of Restorative Dentistry Division of Endodontics Piracicaba Dental School State University of Campinas – UNICAMP, Av. Limeira 901. Bairro AreaoFaculty of Medical Sciences University Center Lusíada – UNILUSDepartment of Operative Dentistry Division of Endodontics The University of São Paulo School of Dentistry – FOUSPDepartment of Semiology and Clinic Division of Endodontics School of Dentistry Federal University of Pelotas – UFPELDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESPDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESPUniversidade Estadual de Campinas (UNICAMP)University Center Lusíada – UNILUSUniversidade de São Paulo (USP)Universidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Gomes, Brenda P. F. A.Bronzato, Juliana D.Almeida-Gomes, Rebecca F.Pinheiro, Ericka T.Sousa, Ezilmara L. R.Jacinto, Rogério C. [UNESP]2021-06-25T10:58:14Z2021-06-25T10:58:14Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-021-03923-7Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/20762010.1007/s00784-021-03923-72-s2.0-85104392100Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-19T18:31:36Zoai:repositorio.unesp.br:11449/207620Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
title Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
spellingShingle Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
Gomes, Brenda P. F. A.
Culture
Endodontics
Fusobacterium nucleatum
PCR
title_short Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
title_full Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
title_fullStr Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
title_full_unstemmed Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
title_sort Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
author Gomes, Brenda P. F. A.
author_facet Gomes, Brenda P. F. A.
Bronzato, Juliana D.
Almeida-Gomes, Rebecca F.
Pinheiro, Ericka T.
Sousa, Ezilmara L. R.
Jacinto, Rogério C. [UNESP]
author_role author
author2 Bronzato, Juliana D.
Almeida-Gomes, Rebecca F.
Pinheiro, Ericka T.
Sousa, Ezilmara L. R.
Jacinto, Rogério C. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
University Center Lusíada – UNILUS
Universidade de São Paulo (USP)
Universidade Federal de Pernambuco (UFPE)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gomes, Brenda P. F. A.
Bronzato, Juliana D.
Almeida-Gomes, Rebecca F.
Pinheiro, Ericka T.
Sousa, Ezilmara L. R.
Jacinto, Rogério C. [UNESP]
dc.subject.por.fl_str_mv Culture
Endodontics
Fusobacterium nucleatum
PCR
topic Culture
Endodontics
Fusobacterium nucleatum
PCR
description Objective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:58:14Z
2021-06-25T10:58:14Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-021-03923-7
Clinical Oral Investigations.
1436-3771
1432-6981
http://hdl.handle.net/11449/207620
10.1007/s00784-021-03923-7
2-s2.0-85104392100
url http://dx.doi.org/10.1007/s00784-021-03923-7
http://hdl.handle.net/11449/207620
identifier_str_mv Clinical Oral Investigations.
1436-3771
1432-6981
10.1007/s00784-021-03923-7
2-s2.0-85104392100
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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