Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-021-03923-7 http://hdl.handle.net/11449/207620 |
Resumo: | Objective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination. |
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Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methodsCultureEndodonticsFusobacterium nucleatumPCRObjective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.Department of Restorative Dentistry Division of Endodontics Piracicaba Dental School State University of Campinas – UNICAMP, Av. Limeira 901. Bairro AreaoFaculty of Medical Sciences University Center Lusíada – UNILUSDepartment of Operative Dentistry Division of Endodontics The University of São Paulo School of Dentistry – FOUSPDepartment of Semiology and Clinic Division of Endodontics School of Dentistry Federal University of Pelotas – UFPELDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESPDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESPUniversidade Estadual de Campinas (UNICAMP)University Center Lusíada – UNILUSUniversidade de São Paulo (USP)Universidade Federal de Pernambuco (UFPE)Universidade Estadual Paulista (Unesp)Gomes, Brenda P. F. A.Bronzato, Juliana D.Almeida-Gomes, Rebecca F.Pinheiro, Ericka T.Sousa, Ezilmara L. R.Jacinto, Rogério C. [UNESP]2021-06-25T10:58:14Z2021-06-25T10:58:14Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-021-03923-7Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/20762010.1007/s00784-021-03923-72-s2.0-85104392100Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2024-09-19T18:31:36Zoai:repositorio.unesp.br:11449/207620Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:31:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
title |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
spellingShingle |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods Gomes, Brenda P. F. A. Culture Endodontics Fusobacterium nucleatum PCR |
title_short |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
title_full |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
title_fullStr |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
title_full_unstemmed |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
title_sort |
Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods |
author |
Gomes, Brenda P. F. A. |
author_facet |
Gomes, Brenda P. F. A. Bronzato, Juliana D. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L. R. Jacinto, Rogério C. [UNESP] |
author_role |
author |
author2 |
Bronzato, Juliana D. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L. R. Jacinto, Rogério C. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) University Center Lusíada – UNILUS Universidade de São Paulo (USP) Universidade Federal de Pernambuco (UFPE) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gomes, Brenda P. F. A. Bronzato, Juliana D. Almeida-Gomes, Rebecca F. Pinheiro, Ericka T. Sousa, Ezilmara L. R. Jacinto, Rogério C. [UNESP] |
dc.subject.por.fl_str_mv |
Culture Endodontics Fusobacterium nucleatum PCR |
topic |
Culture Endodontics Fusobacterium nucleatum PCR |
description |
Objective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:58:14Z 2021-06-25T10:58:14Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-021-03923-7 Clinical Oral Investigations. 1436-3771 1432-6981 http://hdl.handle.net/11449/207620 10.1007/s00784-021-03923-7 2-s2.0-85104392100 |
url |
http://dx.doi.org/10.1007/s00784-021-03923-7 http://hdl.handle.net/11449/207620 |
identifier_str_mv |
Clinical Oral Investigations. 1436-3771 1432-6981 10.1007/s00784-021-03923-7 2-s2.0-85104392100 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546441571827712 |