Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascitechnol.v41i1.39128 http://hdl.handle.net/11449/194969 |
Resumo: | Granulated sludge from anaerobic reactors is constituted by the microbial consortia responsible for the degradation of different substrate present in wastewaters. This study characterized anaerobic microorganisms in a granular sludge from a Uasb reactor (Upflow Anaerobic Sludge Blanket) by Most Probable Number (MPN) technique and method of cloning and sequencing the 16S rDNA gene. The main objective of this study was to quantify and to identify the microorganisms in two different culture media containing sulfate (Posgate C [6.0 sodium lactate and 3.13 g L-1 sodium sulfate] and Zinder [2.24 sodium lactate and 0.96 g L-1 sodium sulfate]). Microorganisms quantified by Postgate C and Zinder media were: 9.30x10(10) and 7.50x10(11) MPN mL(-1) for general anaerobic bacteria; 4.30x10(8) and 2.10x10(8) MPN mL(-1) for sulfate reducing bacteria; and 1.20x10(8) and 1.5x10(8) MPN mL(-1) for methane producing archaea, respectively. Bacteria identified by 16S rDNA gene sequencing from the MPN in both culture media were related to the phyla Firmicutes and Proteobacteria. The conditions of Postgate C culture medium favored the sulfate-reducing bacteria and the Zinder culture medium favored the general anaerobic bacteria. |
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Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditionssulfate reducing bacteriamethane producing archaea16S rDNA genecloning/sequencingupflow anaerobic sludge blanket reactorGranulated sludge from anaerobic reactors is constituted by the microbial consortia responsible for the degradation of different substrate present in wastewaters. This study characterized anaerobic microorganisms in a granular sludge from a Uasb reactor (Upflow Anaerobic Sludge Blanket) by Most Probable Number (MPN) technique and method of cloning and sequencing the 16S rDNA gene. The main objective of this study was to quantify and to identify the microorganisms in two different culture media containing sulfate (Posgate C [6.0 sodium lactate and 3.13 g L-1 sodium sulfate] and Zinder [2.24 sodium lactate and 0.96 g L-1 sodium sulfate]). Microorganisms quantified by Postgate C and Zinder media were: 9.30x10(10) and 7.50x10(11) MPN mL(-1) for general anaerobic bacteria; 4.30x10(8) and 2.10x10(8) MPN mL(-1) for sulfate reducing bacteria; and 1.20x10(8) and 1.5x10(8) MPN mL(-1) for methane producing archaea, respectively. Bacteria identified by 16S rDNA gene sequencing from the MPN in both culture media were related to the phyla Firmicutes and Proteobacteria. The conditions of Postgate C culture medium favored the sulfate-reducing bacteria and the Zinder culture medium favored the general anaerobic bacteria.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian Innovation Agency (Finep)Univ Sao Paulo, Dept Hidraul & Saneamento, Escola Engn Sao Carlos, Ave Joao Dagnone 1100, BR-13563120 Sao Paulo, SP, BrazilUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, BrazilUniv Estadual Paulista, Inst Pesquisa Bioenergia, Rio Claro, SP, BrazilUniv Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacaoUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Sakamoto, Isabel KimikoMaintiguer, Sandra Imaculada [UNESP]Amancio Varesche, Maria Bernadete2020-12-10T17:00:14Z2020-12-10T17:00:14Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.4025/actascitechnol.v41i1.39128Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 41, 11 p., 2019.1806-2563http://hdl.handle.net/11449/19496910.4025/actascitechnol.v41i1.39128WOS:000496531700034Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Scientiarum-technologyinfo:eu-repo/semantics/openAccess2021-10-23T03:12:11Zoai:repositorio.unesp.br:11449/194969Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:04:46.357685Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
title |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
spellingShingle |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions Sakamoto, Isabel Kimiko sulfate reducing bacteria methane producing archaea 16S rDNA gene cloning/sequencing upflow anaerobic sludge blanket reactor |
title_short |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
title_full |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
title_fullStr |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
title_full_unstemmed |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
title_sort |
Phylogenetic characterization and quantification by Most Probable Number of the microbial communities of biomass from the Upflow Anaerobic Sludge Blanket Reactor under sulfidogenic conditions |
author |
Sakamoto, Isabel Kimiko |
author_facet |
Sakamoto, Isabel Kimiko Maintiguer, Sandra Imaculada [UNESP] Amancio Varesche, Maria Bernadete |
author_role |
author |
author2 |
Maintiguer, Sandra Imaculada [UNESP] Amancio Varesche, Maria Bernadete |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sakamoto, Isabel Kimiko Maintiguer, Sandra Imaculada [UNESP] Amancio Varesche, Maria Bernadete |
dc.subject.por.fl_str_mv |
sulfate reducing bacteria methane producing archaea 16S rDNA gene cloning/sequencing upflow anaerobic sludge blanket reactor |
topic |
sulfate reducing bacteria methane producing archaea 16S rDNA gene cloning/sequencing upflow anaerobic sludge blanket reactor |
description |
Granulated sludge from anaerobic reactors is constituted by the microbial consortia responsible for the degradation of different substrate present in wastewaters. This study characterized anaerobic microorganisms in a granular sludge from a Uasb reactor (Upflow Anaerobic Sludge Blanket) by Most Probable Number (MPN) technique and method of cloning and sequencing the 16S rDNA gene. The main objective of this study was to quantify and to identify the microorganisms in two different culture media containing sulfate (Posgate C [6.0 sodium lactate and 3.13 g L-1 sodium sulfate] and Zinder [2.24 sodium lactate and 0.96 g L-1 sodium sulfate]). Microorganisms quantified by Postgate C and Zinder media were: 9.30x10(10) and 7.50x10(11) MPN mL(-1) for general anaerobic bacteria; 4.30x10(8) and 2.10x10(8) MPN mL(-1) for sulfate reducing bacteria; and 1.20x10(8) and 1.5x10(8) MPN mL(-1) for methane producing archaea, respectively. Bacteria identified by 16S rDNA gene sequencing from the MPN in both culture media were related to the phyla Firmicutes and Proteobacteria. The conditions of Postgate C culture medium favored the sulfate-reducing bacteria and the Zinder culture medium favored the general anaerobic bacteria. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-10T17:00:14Z 2020-12-10T17:00:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascitechnol.v41i1.39128 Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 41, 11 p., 2019. 1806-2563 http://hdl.handle.net/11449/194969 10.4025/actascitechnol.v41i1.39128 WOS:000496531700034 |
url |
http://dx.doi.org/10.4025/actascitechnol.v41i1.39128 http://hdl.handle.net/11449/194969 |
identifier_str_mv |
Acta Scientiarum-technology. Maringa: Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao, v. 41, 11 p., 2019. 1806-2563 10.4025/actascitechnol.v41i1.39128 WOS:000496531700034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Scientiarum-technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao |
publisher.none.fl_str_mv |
Univ Estadual Maringa, Pro-reitoria Pesquisa Pos-graduacao |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129580057231360 |