Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/231815 |
Resumo: | Objective: To evaluate the differences between three methods for the measurement of experimental infarction in rats in comparison to the traditional method. Methods: Histological analysis of the infarction area (AREA), histological analysis of the internal cavity perimeter (PER) and echocardiogram analysis of the internal perimeter (ECHO) were compared to the traditional method (histological analysis of the epicardial and endocardial circumferences of the infarction region - CIR). Repeated ANOVA measurements were used in conjunction with the Dunn multiple comparison test, the Bland and Altman concordance method and the Spearman correlation test. Significance was established as p < 0.05. Results: The data of 122 animals were analyzed, 3 to 6 months after the infarction. Infarction size assessments revealed differences between CIR and the other three methods (p < 0.001): CIR = 42.4% (35.9-48.8), PER = 50.3% (39.1-57.0), AREA = 27.3% (20.2-34.3), ECHO = 46.1% (39.9-52.6). Therefore, measurement by area underestimated the infarct size by 15%, whereas the echocardiogram and histological internal perimeter measurements overestimated the infarct size by 4% and 5%, respectively. In relation to ECHO and PER, even though the difference between the methods was only 1.27%, the concordance interval ranged from 24.1% to -26.7%, suggesting a low level of concordance between the methods. In relation to associations, statistically significant correlations were found between: CIR and PER (r = 0.88 and p < 0.0001); CIR and AREA (r = 0.87 and p < 0.0001) and CIR and ECHO (r = 0.42 and p < 0.0001). Conclusion: Despite the high level of correlation, there was a low level of concordance between the methods to define infarct size. |
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Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratosComparison of different methods to measure experimental chronic infarction size in the rat modelMyocardial infarction/physiopatologyRatsObjective: To evaluate the differences between three methods for the measurement of experimental infarction in rats in comparison to the traditional method. Methods: Histological analysis of the infarction area (AREA), histological analysis of the internal cavity perimeter (PER) and echocardiogram analysis of the internal perimeter (ECHO) were compared to the traditional method (histological analysis of the epicardial and endocardial circumferences of the infarction region - CIR). Repeated ANOVA measurements were used in conjunction with the Dunn multiple comparison test, the Bland and Altman concordance method and the Spearman correlation test. Significance was established as p < 0.05. Results: The data of 122 animals were analyzed, 3 to 6 months after the infarction. Infarction size assessments revealed differences between CIR and the other three methods (p < 0.001): CIR = 42.4% (35.9-48.8), PER = 50.3% (39.1-57.0), AREA = 27.3% (20.2-34.3), ECHO = 46.1% (39.9-52.6). Therefore, measurement by area underestimated the infarct size by 15%, whereas the echocardiogram and histological internal perimeter measurements overestimated the infarct size by 4% and 5%, respectively. In relation to ECHO and PER, even though the difference between the methods was only 1.27%, the concordance interval ranged from 24.1% to -26.7%, suggesting a low level of concordance between the methods. In relation to associations, statistically significant correlations were found between: CIR and PER (r = 0.88 and p < 0.0001); CIR and AREA (r = 0.87 and p < 0.0001) and CIR and ECHO (r = 0.42 and p < 0.0001). Conclusion: Despite the high level of correlation, there was a low level of concordance between the methods to define infarct size.Faculdade de Medicina de Botucatu UNESP, Botucatu, SPFaculdade de Medicina de Botucatu Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, 18618-000 - Botucatu, SPFaculdade de Medicina de Botucatu UNESP, Botucatu, SPFaculdade de Medicina de Botucatu Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, 18618-000 - Botucatu, SPUniversidade Estadual Paulista (UNESP)Minicucci, Marcos F. [UNESP]Azevedo, Paula S. [UNESP]Duarte, Daniella R. [UNESP]Matsubara, Beatriz B. [UNESP]Matsubara, Luiz S. [UNESP]Campana, Álvaro O. [UNESP]Paiva, Sergio A. R. [UNESP]Zornoff, Leonardo A. M. [UNESP]2022-04-29T08:47:41Z2022-04-29T08:47:41Z2007-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleArquivos Brasileiros de Cardiologia, v. 89, n. 2, 2007.0066-782X1678-4170http://hdl.handle.net/11449/2318152-s2.0-34548816988Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporengArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2022-04-29T08:47:41Zoai:repositorio.unesp.br:11449/231815Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:47:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos Comparison of different methods to measure experimental chronic infarction size in the rat model |
title |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
spellingShingle |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos Minicucci, Marcos F. [UNESP] Myocardial infarction/physiopatology Rats |
title_short |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
title_full |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
title_fullStr |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
title_full_unstemmed |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
title_sort |
Comparação de diferentes métodos para medida do tamanho do infarto experimental crônico em ratos |
author |
Minicucci, Marcos F. [UNESP] |
author_facet |
Minicucci, Marcos F. [UNESP] Azevedo, Paula S. [UNESP] Duarte, Daniella R. [UNESP] Matsubara, Beatriz B. [UNESP] Matsubara, Luiz S. [UNESP] Campana, Álvaro O. [UNESP] Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] |
author_role |
author |
author2 |
Azevedo, Paula S. [UNESP] Duarte, Daniella R. [UNESP] Matsubara, Beatriz B. [UNESP] Matsubara, Luiz S. [UNESP] Campana, Álvaro O. [UNESP] Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Minicucci, Marcos F. [UNESP] Azevedo, Paula S. [UNESP] Duarte, Daniella R. [UNESP] Matsubara, Beatriz B. [UNESP] Matsubara, Luiz S. [UNESP] Campana, Álvaro O. [UNESP] Paiva, Sergio A. R. [UNESP] Zornoff, Leonardo A. M. [UNESP] |
dc.subject.por.fl_str_mv |
Myocardial infarction/physiopatology Rats |
topic |
Myocardial infarction/physiopatology Rats |
description |
Objective: To evaluate the differences between three methods for the measurement of experimental infarction in rats in comparison to the traditional method. Methods: Histological analysis of the infarction area (AREA), histological analysis of the internal cavity perimeter (PER) and echocardiogram analysis of the internal perimeter (ECHO) were compared to the traditional method (histological analysis of the epicardial and endocardial circumferences of the infarction region - CIR). Repeated ANOVA measurements were used in conjunction with the Dunn multiple comparison test, the Bland and Altman concordance method and the Spearman correlation test. Significance was established as p < 0.05. Results: The data of 122 animals were analyzed, 3 to 6 months after the infarction. Infarction size assessments revealed differences between CIR and the other three methods (p < 0.001): CIR = 42.4% (35.9-48.8), PER = 50.3% (39.1-57.0), AREA = 27.3% (20.2-34.3), ECHO = 46.1% (39.9-52.6). Therefore, measurement by area underestimated the infarct size by 15%, whereas the echocardiogram and histological internal perimeter measurements overestimated the infarct size by 4% and 5%, respectively. In relation to ECHO and PER, even though the difference between the methods was only 1.27%, the concordance interval ranged from 24.1% to -26.7%, suggesting a low level of concordance between the methods. In relation to associations, statistically significant correlations were found between: CIR and PER (r = 0.88 and p < 0.0001); CIR and AREA (r = 0.87 and p < 0.0001) and CIR and ECHO (r = 0.42 and p < 0.0001). Conclusion: Despite the high level of correlation, there was a low level of concordance between the methods to define infarct size. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-01 2022-04-29T08:47:41Z 2022-04-29T08:47:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Arquivos Brasileiros de Cardiologia, v. 89, n. 2, 2007. 0066-782X 1678-4170 http://hdl.handle.net/11449/231815 2-s2.0-34548816988 |
identifier_str_mv |
Arquivos Brasileiros de Cardiologia, v. 89, n. 2, 2007. 0066-782X 1678-4170 2-s2.0-34548816988 |
url |
http://hdl.handle.net/11449/231815 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964961348255744 |