Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00011-022-01553-7 http://hdl.handle.net/11449/230663 |
Resumo: | Objective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis. |
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Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in ratsAndrogensGranulation tissueInflammationRatsSkinTestosteroneObjective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Stomatology Federal University of Paraná UFPR, Pref. Lothário Meissner Av., 632 – Center, PRDepartment of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPFAPESP: 2015/20281-0Universidade Estadual Paulista (UNESP)Universidade Federal do Paraná (UFPR)de Paiva Gonçalves, Vinícius [UNESP]Steffens, João PauloJunior, Carlos Rossa [UNESP]Spolidorio, Luís Carlos [UNESP]2022-04-29T08:41:25Z2022-04-29T08:41:25Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article473-483http://dx.doi.org/10.1007/s00011-022-01553-7Inflammation Research, v. 71, n. 4, p. 473-483, 2022.1420-908X1023-3830http://hdl.handle.net/11449/23066310.1007/s00011-022-01553-72-s2.0-85127416418Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammation Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:06:00Zoai:repositorio.unesp.br:11449/230663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
title |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
spellingShingle |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats de Paiva Gonçalves, Vinícius [UNESP] Androgens Granulation tissue Inflammation Rats Skin Testosterone |
title_short |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
title_full |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
title_fullStr |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
title_full_unstemmed |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
title_sort |
Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats |
author |
de Paiva Gonçalves, Vinícius [UNESP] |
author_facet |
de Paiva Gonçalves, Vinícius [UNESP] Steffens, João Paulo Junior, Carlos Rossa [UNESP] Spolidorio, Luís Carlos [UNESP] |
author_role |
author |
author2 |
Steffens, João Paulo Junior, Carlos Rossa [UNESP] Spolidorio, Luís Carlos [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal do Paraná (UFPR) |
dc.contributor.author.fl_str_mv |
de Paiva Gonçalves, Vinícius [UNESP] Steffens, João Paulo Junior, Carlos Rossa [UNESP] Spolidorio, Luís Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Androgens Granulation tissue Inflammation Rats Skin Testosterone |
topic |
Androgens Granulation tissue Inflammation Rats Skin Testosterone |
description |
Objective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:41:25Z 2022-04-29T08:41:25Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00011-022-01553-7 Inflammation Research, v. 71, n. 4, p. 473-483, 2022. 1420-908X 1023-3830 http://hdl.handle.net/11449/230663 10.1007/s00011-022-01553-7 2-s2.0-85127416418 |
url |
http://dx.doi.org/10.1007/s00011-022-01553-7 http://hdl.handle.net/11449/230663 |
identifier_str_mv |
Inflammation Research, v. 71, n. 4, p. 473-483, 2022. 1420-908X 1023-3830 10.1007/s00011-022-01553-7 2-s2.0-85127416418 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Inflammation Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
473-483 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546537609854976 |