Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats

Detalhes bibliográficos
Autor(a) principal: de Paiva Gonçalves, Vinícius [UNESP]
Data de Publicação: 2022
Outros Autores: Steffens, João Paulo, Junior, Carlos Rossa [UNESP], Spolidorio, Luís Carlos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00011-022-01553-7
http://hdl.handle.net/11449/230663
Resumo: Objective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis.
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spelling Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in ratsAndrogensGranulation tissueInflammationRatsSkinTestosteroneObjective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Stomatology Federal University of Paraná UFPR, Pref. Lothário Meissner Av., 632 – Center, PRDepartment of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Physiology and Pathology Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPDepartment of Diagnosis and Surgery Araraquara School of Dentistry University of São Paulo State UNESP, Humaitá St., 1680 – Center, SPFAPESP: 2015/20281-0Universidade Estadual Paulista (UNESP)Universidade Federal do Paraná (UFPR)de Paiva Gonçalves, Vinícius [UNESP]Steffens, João PauloJunior, Carlos Rossa [UNESP]Spolidorio, Luís Carlos [UNESP]2022-04-29T08:41:25Z2022-04-29T08:41:25Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article473-483http://dx.doi.org/10.1007/s00011-022-01553-7Inflammation Research, v. 71, n. 4, p. 473-483, 2022.1420-908X1023-3830http://hdl.handle.net/11449/23066310.1007/s00011-022-01553-72-s2.0-85127416418Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInflammation Researchinfo:eu-repo/semantics/openAccess2024-09-27T14:06:00Zoai:repositorio.unesp.br:11449/230663Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
title Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
spellingShingle Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
de Paiva Gonçalves, Vinícius [UNESP]
Androgens
Granulation tissue
Inflammation
Rats
Skin
Testosterone
title_short Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
title_full Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
title_fullStr Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
title_full_unstemmed Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
title_sort Supraphysiological testosterone supplementation improves granulation tissue maturation through angiogenesis in the early phase of a cutaneous wound healing model in rats
author de Paiva Gonçalves, Vinícius [UNESP]
author_facet de Paiva Gonçalves, Vinícius [UNESP]
Steffens, João Paulo
Junior, Carlos Rossa [UNESP]
Spolidorio, Luís Carlos [UNESP]
author_role author
author2 Steffens, João Paulo
Junior, Carlos Rossa [UNESP]
Spolidorio, Luís Carlos [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal do Paraná (UFPR)
dc.contributor.author.fl_str_mv de Paiva Gonçalves, Vinícius [UNESP]
Steffens, João Paulo
Junior, Carlos Rossa [UNESP]
Spolidorio, Luís Carlos [UNESP]
dc.subject.por.fl_str_mv Androgens
Granulation tissue
Inflammation
Rats
Skin
Testosterone
topic Androgens
Granulation tissue
Inflammation
Rats
Skin
Testosterone
description Objective: The aim of this study was to evaluate the effects of both testosterone depletion and supraphysiological testosterone supplementation in the early phase of an animal cutaneous wound healing model in comparison with the physiological hormonal condition. Material and Methods: Forty rats were distributed into the following four groups: Control, Orchiectomy (OCX), Durateston (Dura) and OCX+Dura. On day 1, the testicles were removed (OCX group) and the rats (Dura group) received a supraphysiological dose (250 mg/kg) of exogenous testosterone weekly. After 15 days a full-thickness excisional skin wound was created in all animals, which was healed by the second intention for 7 days. On day 22, the rats were euthanatized and the wounds were harvested for histopathological evaluation, immunohistochemistry analyses and multiplex immunoassay. One-way ANOVA and post-hoc Tukey tests were performed. Results: It was found that the supraphysiological testosterone level increased extracellular matrix deposition, promoted higher blood vessel formation and reduced wound contraction (p < 0.05). Additionally, it also stimulated PCNA-positive fibroblasts and KGF-positive cells (p < 0.05), while orchiectomy reduced the expression of IL-6 and TNF-α and increased VEGF and PDGF (p < 0.05). Conclusion: In conclusion, the results provide evidence that supraphysiological testosterone supplementation plays a positive role in the early phase of cutaneous wound healing, thus improving granulation tissue maturation through the enhancement of angiogenesis.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:41:25Z
2022-04-29T08:41:25Z
2022-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00011-022-01553-7
Inflammation Research, v. 71, n. 4, p. 473-483, 2022.
1420-908X
1023-3830
http://hdl.handle.net/11449/230663
10.1007/s00011-022-01553-7
2-s2.0-85127416418
url http://dx.doi.org/10.1007/s00011-022-01553-7
http://hdl.handle.net/11449/230663
identifier_str_mv Inflammation Research, v. 71, n. 4, p. 473-483, 2022.
1420-908X
1023-3830
10.1007/s00011-022-01553-7
2-s2.0-85127416418
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Inflammation Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 473-483
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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