Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2018.133937 http://hdl.handle.net/11449/231434 |
Resumo: | Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E2) but not the CL (local P4), and both PDBu and OT stimulated PGF2a synthesis. |
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Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattleProdução in vitro de pgf f2α endometrial e sua modulação referente ao folículo dominante em bovinosCattleLuteolysisPGF2α synthesisReproductive physiologyProstaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E2) but not the CL (local P4), and both PDBu and OT stimulated PGF2a synthesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of FloridaUniversidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Reprodução AnimalUniversidade de São Paulo Faculdade de Zootecnia e Engenharia de Alimentos da USP Departamento de Medicina VeterináriaUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e TecnológicasUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agrárias e TecnológicasFAPESP: 03/01584-4FAPESP: 99/03383-9Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Bressan, Fabiana FernandesMembrive, Claudia Maria Bertan [UNESP]Goissis, Marcelo DemarchiMarques, Vanessa Belentanida CUNHA, Pauline MartinsBinelli, Mario2022-04-29T08:45:25Z2022-04-29T08:45:25Z2019-07-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.11606/issn.1678-4456.bjvras.2018.133937Brazilian Journal of Veterinary Research and Animal Science, v. 55, n. 2, 2019.1678-44561413-9596http://hdl.handle.net/11449/23143410.11606/issn.1678-4456.bjvras.2018.1339372-s2.0-85067615059Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:45:25Zoai:repositorio.unesp.br:11449/231434Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:45:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle Produção in vitro de pgf f2α endometrial e sua modulação referente ao folículo dominante em bovinos |
title |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
spellingShingle |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle Bressan, Fabiana Fernandes Cattle Luteolysis PGF2α synthesis Reproductive physiology |
title_short |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
title_full |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
title_fullStr |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
title_full_unstemmed |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
title_sort |
Endometrial prostaglandin f2α in vitro production and its modulation regarding dominant follicle position in cattle |
author |
Bressan, Fabiana Fernandes |
author_facet |
Bressan, Fabiana Fernandes Membrive, Claudia Maria Bertan [UNESP] Goissis, Marcelo Demarchi Marques, Vanessa Belentani da CUNHA, Pauline Martins Binelli, Mario |
author_role |
author |
author2 |
Membrive, Claudia Maria Bertan [UNESP] Goissis, Marcelo Demarchi Marques, Vanessa Belentani da CUNHA, Pauline Martins Binelli, Mario |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Bressan, Fabiana Fernandes Membrive, Claudia Maria Bertan [UNESP] Goissis, Marcelo Demarchi Marques, Vanessa Belentani da CUNHA, Pauline Martins Binelli, Mario |
dc.subject.por.fl_str_mv |
Cattle Luteolysis PGF2α synthesis Reproductive physiology |
topic |
Cattle Luteolysis PGF2α synthesis Reproductive physiology |
description |
Prostaglandin F2α (PGF2α) determines luteolysis in cattle, and the ability to manipulate its endogenous synthesis is indispensible for large-scale animal breeding. Estradiol (E2) and progesterone (P4) modulate several molecular pathways in endometrial cells, including the synthesis of PGF2α; however, its specific mechanisms are still not totally known. This study investigated the production in vitro and possible modulation of endometrial PGF2α due to a local effect of endogenous E2 in the ipsilateral uterine horn (UH) containing the dominant follicle (DF) or from P4 in ipsilateral horn containing the corpus luteum (CL). The PGF2α stimulators oxytocin (OT) and phorbol 12,13-dibutyrate (PDBu) were incubated with endometrial explants, and PGF2α content was measured. For that, cycling cows were synchronized, the development of DF and CL was examined by ultrasonography and on the seventh day of the estrous cycle, endometrial explants were collected and cultured in medium supplemented with 10-6 M PDBu or 10-6 M OT or non-supplemented. Media samples were collected immediately after treatment and 60 min later. Radioimmunoassay showed that the PGF2α content of the UH ipsilateral to the DF was 49% less than that of the contralateral UH (8.22 ± 0.95 vs. 12.24 ± 0.95 pg/mL/mg tissue, respectively; P < 0.01). However, the PGF2α levels did not differ between the UHs as a function of the CL position (9.46 ± 0.95 vs. 11 ± 0.95 pg/mL/mg; P > 0.05). The cellular stimulators promoted an increase in PGF2α synthesis (P < 0.02), and the effects differed among the animals (P < 0.04). The PGF2a production was higher in the explants treated with PDBu rather than OT (13.68 ± 1.16 vs. 10.01 ± 1.16 pg/mL/mg tissue, respectively; P < 0.05). In conclusion, PGF2α synthesis is modulated by the presence of the DF (local E2) but not the CL (local P4), and both PDBu and OT stimulated PGF2a synthesis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-26 2022-04-29T08:45:25Z 2022-04-29T08:45:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2018.133937 Brazilian Journal of Veterinary Research and Animal Science, v. 55, n. 2, 2019. 1678-4456 1413-9596 http://hdl.handle.net/11449/231434 10.11606/issn.1678-4456.bjvras.2018.133937 2-s2.0-85067615059 |
url |
http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2018.133937 http://hdl.handle.net/11449/231434 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 55, n. 2, 2019. 1678-4456 1413-9596 10.11606/issn.1678-4456.bjvras.2018.133937 2-s2.0-85067615059 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965185618739200 |