Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jphotobiol.2016.02.036 http://hdl.handle.net/11449/168504 |
Resumo: | The occurrence of a variety of pathogens resistant to current antibiotics remains the major problem in medical care, especially when bacterial infections are established as biofilms. In this study, we propose the use of photodynamic therapy (PDT) as a monotherapy and associated with antibiotic as an alternative treatment. The aim of this study was to analyze the effects of PDT mediated by methylene blue (MB) on Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in both biofilm and planktonic phases. Several concentrations of MB and light doses were tested. The bactericidal effects of PDT as a monotherapy did not increase with the concentration of photosensitizer, but were light dose-dependent. In addition, bacteria in biofilms were less affected than cells in the planktonic phase. Although not concentration-dependent, the disruption effect of PDT on biofilms was clearly illustrated by scanning electron microscopy (SEM). We also carried out experiments that evaluated the synergistic effect of photodynamic therapy and the antibiotic ciprofloxacin. The best results were obtained after combination treatment of photodynamic therapy followed by ciprofloxacin on biofilms, which increased bacterial reduction on biofilms, resulting in a 5.4 log reduction for S. aureus biofilm and approximately 7 log for E. coli biofilm. |
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Repositório Institucional da UNESP |
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Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacinCombined therapyEscherichia coliMethylene bluePhotodynamic therapyStaphylococcus aureusThe occurrence of a variety of pathogens resistant to current antibiotics remains the major problem in medical care, especially when bacterial infections are established as biofilms. In this study, we propose the use of photodynamic therapy (PDT) as a monotherapy and associated with antibiotic as an alternative treatment. The aim of this study was to analyze the effects of PDT mediated by methylene blue (MB) on Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in both biofilm and planktonic phases. Several concentrations of MB and light doses were tested. The bactericidal effects of PDT as a monotherapy did not increase with the concentration of photosensitizer, but were light dose-dependent. In addition, bacteria in biofilms were less affected than cells in the planktonic phase. Although not concentration-dependent, the disruption effect of PDT on biofilms was clearly illustrated by scanning electron microscopy (SEM). We also carried out experiments that evaluated the synergistic effect of photodynamic therapy and the antibiotic ciprofloxacin. The best results were obtained after combination treatment of photodynamic therapy followed by ciprofloxacin on biofilms, which increased bacterial reduction on biofilms, resulting in a 5.4 log reduction for S. aureus biofilm and approximately 7 log for E. coli biofilm.Faculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP) Campos Ville, Rodovia Araraquara-Jau, km 01 - s/nFaculdade de Ciencias Farmaceuticas Univ. Estadual Paulista (UNESP) Campos Ville, Rodovia Araraquara-Jau, km 01 - s/nUniversidade Estadual Paulista (Unesp)Ronqui, Maria Rita [UNESP]De Aguiar Coletti, Tatiana Maria Starck Fogaça [UNESP]De Freitas, Laura Marise [UNESP]Miranda, Elaine Toscano [UNESP]Fontana, Carla Raquel [UNESP]2018-12-11T16:41:33Z2018-12-11T16:41:33Z2016-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article122-129application/pdfhttp://dx.doi.org/10.1016/j.jphotobiol.2016.02.036Journal of Photochemistry and Photobiology B: Biology, v. 158, p. 122-129.1873-26821011-1344http://hdl.handle.net/11449/16850410.1016/j.jphotobiol.2016.02.0362-s2.0-849616160632-s2.0-84961616063.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Photochemistry and Photobiology B: Biology0,698info:eu-repo/semantics/openAccess2023-10-01T06:04:14Zoai:repositorio.unesp.br:11449/168504Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:39:52.861860Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
title |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
spellingShingle |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin Ronqui, Maria Rita [UNESP] Combined therapy Escherichia coli Methylene blue Photodynamic therapy Staphylococcus aureus |
title_short |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
title_full |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
title_fullStr |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
title_full_unstemmed |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
title_sort |
Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin |
author |
Ronqui, Maria Rita [UNESP] |
author_facet |
Ronqui, Maria Rita [UNESP] De Aguiar Coletti, Tatiana Maria Starck Fogaça [UNESP] De Freitas, Laura Marise [UNESP] Miranda, Elaine Toscano [UNESP] Fontana, Carla Raquel [UNESP] |
author_role |
author |
author2 |
De Aguiar Coletti, Tatiana Maria Starck Fogaça [UNESP] De Freitas, Laura Marise [UNESP] Miranda, Elaine Toscano [UNESP] Fontana, Carla Raquel [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ronqui, Maria Rita [UNESP] De Aguiar Coletti, Tatiana Maria Starck Fogaça [UNESP] De Freitas, Laura Marise [UNESP] Miranda, Elaine Toscano [UNESP] Fontana, Carla Raquel [UNESP] |
dc.subject.por.fl_str_mv |
Combined therapy Escherichia coli Methylene blue Photodynamic therapy Staphylococcus aureus |
topic |
Combined therapy Escherichia coli Methylene blue Photodynamic therapy Staphylococcus aureus |
description |
The occurrence of a variety of pathogens resistant to current antibiotics remains the major problem in medical care, especially when bacterial infections are established as biofilms. In this study, we propose the use of photodynamic therapy (PDT) as a monotherapy and associated with antibiotic as an alternative treatment. The aim of this study was to analyze the effects of PDT mediated by methylene blue (MB) on Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) in both biofilm and planktonic phases. Several concentrations of MB and light doses were tested. The bactericidal effects of PDT as a monotherapy did not increase with the concentration of photosensitizer, but were light dose-dependent. In addition, bacteria in biofilms were less affected than cells in the planktonic phase. Although not concentration-dependent, the disruption effect of PDT on biofilms was clearly illustrated by scanning electron microscopy (SEM). We also carried out experiments that evaluated the synergistic effect of photodynamic therapy and the antibiotic ciprofloxacin. The best results were obtained after combination treatment of photodynamic therapy followed by ciprofloxacin on biofilms, which increased bacterial reduction on biofilms, resulting in a 5.4 log reduction for S. aureus biofilm and approximately 7 log for E. coli biofilm. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 2018-12-11T16:41:33Z 2018-12-11T16:41:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jphotobiol.2016.02.036 Journal of Photochemistry and Photobiology B: Biology, v. 158, p. 122-129. 1873-2682 1011-1344 http://hdl.handle.net/11449/168504 10.1016/j.jphotobiol.2016.02.036 2-s2.0-84961616063 2-s2.0-84961616063.pdf |
url |
http://dx.doi.org/10.1016/j.jphotobiol.2016.02.036 http://hdl.handle.net/11449/168504 |
identifier_str_mv |
Journal of Photochemistry and Photobiology B: Biology, v. 158, p. 122-129. 1873-2682 1011-1344 10.1016/j.jphotobiol.2016.02.036 2-s2.0-84961616063 2-s2.0-84961616063.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Photochemistry and Photobiology B: Biology 0,698 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
122-129 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128261018877952 |