Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2016.07.023 http://hdl.handle.net/11449/161922 |
Resumo: | Hemocyanins are large extracellular respiratory proteins distributed within the hemolymph of arthropods, molluscs and larval stages of certain insects. In the present study, we characterized the humoral immune functions of cation specific antibacterial hemocyanin (Ab-Hcy) from mud crab, S. serrata. The bacteriolytic activity of Ab-Hcy was against pathogenic and non-pathogenic to crustaceans which includes, Bacillus sp. N1, B. flexus N3, E. coli, P. aeruginosa, V. harveyi, V. parahaemolyticus and V. vulnificus and also expressed bacteriostatic activity against E. coli, B. flexus N3 and V. harveyi. The agglutination activity of Ab-Hcy ranged from 4-16 against B. flexus N3, E. coli, V. harveyi and V. vulnificus and Ab-Hcy also agglutinated with rat and human O erythrocytes (HA titer: 2). The Ab-Hcy lysed the panel of mammalian erythrocytes and strong hemolytic activity of 1.40, 1.28 and 1.22 units.min(-1) . mg protein(-1) against human A, B and O erythrocytes respectively. It was also found that the Ab-Hcy possess phenoloxidase (PO) activity by oxidizing L-DOPA. These results suggest that Ab-Hcy from the serumofmud crab, S. serrata, is capable of performing multiple humoral immune functions in addition to oxygen transportation. Statement of relevance: This manuscript deals with the humoral immune functions, particularly antibacterial property of hemocyanin in crustacean. In this paper we report the different type of antibacterial activity and other humoral immune functions of a hemocyanin isolated from the serum of mud crab, Scylla serrata. This work will help to understand the role of respiratory molecule in immune system, also will be helpful improve disease free crustacean aquaculture industry. And we also state this research work is relevant and suitable to publish in the Journal Aquaculture. (C) 2016 Elsevier B.V. All rights reserved. |
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Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrataScylla serrataHemocyaninAntibacterialHumoral functionsHemocyanins are large extracellular respiratory proteins distributed within the hemolymph of arthropods, molluscs and larval stages of certain insects. In the present study, we characterized the humoral immune functions of cation specific antibacterial hemocyanin (Ab-Hcy) from mud crab, S. serrata. The bacteriolytic activity of Ab-Hcy was against pathogenic and non-pathogenic to crustaceans which includes, Bacillus sp. N1, B. flexus N3, E. coli, P. aeruginosa, V. harveyi, V. parahaemolyticus and V. vulnificus and also expressed bacteriostatic activity against E. coli, B. flexus N3 and V. harveyi. The agglutination activity of Ab-Hcy ranged from 4-16 against B. flexus N3, E. coli, V. harveyi and V. vulnificus and Ab-Hcy also agglutinated with rat and human O erythrocytes (HA titer: 2). The Ab-Hcy lysed the panel of mammalian erythrocytes and strong hemolytic activity of 1.40, 1.28 and 1.22 units.min(-1) . mg protein(-1) against human A, B and O erythrocytes respectively. It was also found that the Ab-Hcy possess phenoloxidase (PO) activity by oxidizing L-DOPA. These results suggest that Ab-Hcy from the serumofmud crab, S. serrata, is capable of performing multiple humoral immune functions in addition to oxygen transportation. Statement of relevance: This manuscript deals with the humoral immune functions, particularly antibacterial property of hemocyanin in crustacean. In this paper we report the different type of antibacterial activity and other humoral immune functions of a hemocyanin isolated from the serum of mud crab, Scylla serrata. This work will help to understand the role of respiratory molecule in immune system, also will be helpful improve disease free crustacean aquaculture industry. And we also state this research work is relevant and suitable to publish in the Journal Aquaculture. (C) 2016 Elsevier B.V. All rights reserved.University Grants CommissionDepartment of Biotechnology, New Delhi, IndiaUGC, New Delhi, IndiaUniv Madras, Dept Zool, Guindy Campus, Madras 600025, Tamil Nadu, IndiaSao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, Rua Humaita 1680, BR-14801903 Sao Paulo, BrazilSao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, Rua Humaita 1680, BR-14801903 Sao Paulo, BrazilUniversity Grants Commission: 4-3/2006 (BSR)/5-133/2007Department of Biotechnology, New Delhi, India: BT/PR11998/AAQ/03/462/2009Elsevier B.V.Univ MadrasUniversidade Estadual Paulista (Unesp)Velayutham, Meiyalagan [UNESP]Munusamy, Arumugam2018-11-26T17:06:11Z2018-11-26T17:06:11Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article428-433application/pdfhttp://dx.doi.org/10.1016/j.aquaculture.2016.07.023Aquaculture. Amsterdam: Elsevier Science Bv, v. 464, p. 428-433, 2016.0044-8486http://hdl.handle.net/11449/16192210.1016/j.aquaculture.2016.07.023WOS:000383370300056WOS000383370300056.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture1,152info:eu-repo/semantics/openAccess2023-11-05T06:07:22Zoai:repositorio.unesp.br:11449/161922Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:56:41.342629Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
title |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
spellingShingle |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata Velayutham, Meiyalagan [UNESP] Scylla serrata Hemocyanin Antibacterial Humoral functions |
title_short |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
title_full |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
title_fullStr |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
title_full_unstemmed |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
title_sort |
Humoral immune responses of antibacterial hemocyanin (Ab-Hcy) in mud crab, Scylla serrata |
author |
Velayutham, Meiyalagan [UNESP] |
author_facet |
Velayutham, Meiyalagan [UNESP] Munusamy, Arumugam |
author_role |
author |
author2 |
Munusamy, Arumugam |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Univ Madras Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Velayutham, Meiyalagan [UNESP] Munusamy, Arumugam |
dc.subject.por.fl_str_mv |
Scylla serrata Hemocyanin Antibacterial Humoral functions |
topic |
Scylla serrata Hemocyanin Antibacterial Humoral functions |
description |
Hemocyanins are large extracellular respiratory proteins distributed within the hemolymph of arthropods, molluscs and larval stages of certain insects. In the present study, we characterized the humoral immune functions of cation specific antibacterial hemocyanin (Ab-Hcy) from mud crab, S. serrata. The bacteriolytic activity of Ab-Hcy was against pathogenic and non-pathogenic to crustaceans which includes, Bacillus sp. N1, B. flexus N3, E. coli, P. aeruginosa, V. harveyi, V. parahaemolyticus and V. vulnificus and also expressed bacteriostatic activity against E. coli, B. flexus N3 and V. harveyi. The agglutination activity of Ab-Hcy ranged from 4-16 against B. flexus N3, E. coli, V. harveyi and V. vulnificus and Ab-Hcy also agglutinated with rat and human O erythrocytes (HA titer: 2). The Ab-Hcy lysed the panel of mammalian erythrocytes and strong hemolytic activity of 1.40, 1.28 and 1.22 units.min(-1) . mg protein(-1) against human A, B and O erythrocytes respectively. It was also found that the Ab-Hcy possess phenoloxidase (PO) activity by oxidizing L-DOPA. These results suggest that Ab-Hcy from the serumofmud crab, S. serrata, is capable of performing multiple humoral immune functions in addition to oxygen transportation. Statement of relevance: This manuscript deals with the humoral immune functions, particularly antibacterial property of hemocyanin in crustacean. In this paper we report the different type of antibacterial activity and other humoral immune functions of a hemocyanin isolated from the serum of mud crab, Scylla serrata. This work will help to understand the role of respiratory molecule in immune system, also will be helpful improve disease free crustacean aquaculture industry. And we also state this research work is relevant and suitable to publish in the Journal Aquaculture. (C) 2016 Elsevier B.V. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T17:06:11Z 2018-11-26T17:06:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2016.07.023 Aquaculture. Amsterdam: Elsevier Science Bv, v. 464, p. 428-433, 2016. 0044-8486 http://hdl.handle.net/11449/161922 10.1016/j.aquaculture.2016.07.023 WOS:000383370300056 WOS000383370300056.pdf |
url |
http://dx.doi.org/10.1016/j.aquaculture.2016.07.023 http://hdl.handle.net/11449/161922 |
identifier_str_mv |
Aquaculture. Amsterdam: Elsevier Science Bv, v. 464, p. 428-433, 2016. 0044-8486 10.1016/j.aquaculture.2016.07.023 WOS:000383370300056 WOS000383370300056.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture 1,152 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
428-433 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128725733081088 |