Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish

Detalhes bibliográficos
Autor(a) principal: Gravato, Carlos
Data de Publicação: 2021
Outros Autores: Abe, Flávia Renata, de Oliveira, Danielle Palma [UNESP], Soares, Amadeu M. V. M., Domingues, Inês
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-0716-1091-6_10
http://hdl.handle.net/11449/205765
Resumo: Acetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.
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spelling Acetylcholinesterase (AChE) Activity in Embryos of ZebrafishAChEBiomarkersDanio rerioEllman’s techniqueEmbryosNeurotoxicityAcetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.Faculdade de Ciências & CESAM Universidade de LisboaSchool of Pharmaceutical Sciences of Ribeirão Preto University of São PauloDepartment of Biology & CESAM Universidade de Aveiro Campus de SantiagoNational Institute for Alternative Technologies ofremoval of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of ChemistryNational Institute for Alternative Technologies ofremoval of Detection Toxicological Evaluation and Removal of Micropollutants and Radioactives (INCT-DATREM) Unesp Institute of ChemistryUniversidade de LisboaUniversidade de São Paulo (USP)Universidade de AveiroUniversidade Estadual Paulista (Unesp)Gravato, CarlosAbe, Flávia Renatade Oliveira, Danielle Palma [UNESP]Soares, Amadeu M. V. M.Domingues, Inês2021-06-25T10:20:50Z2021-06-25T10:20:50Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart119-124http://dx.doi.org/10.1007/978-1-0716-1091-6_10Methods in Molecular Biology, v. 2240, p. 119-124.1940-60291064-3745http://hdl.handle.net/11449/20576510.1007/978-1-0716-1091-6_102-s2.0-85099678031Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-22T17:11:25Zoai:repositorio.unesp.br:11449/205765Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:01:41.486850Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
title Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
spellingShingle Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
Gravato, Carlos
AChE
Biomarkers
Danio rerio
Ellman’s technique
Embryos
Neurotoxicity
title_short Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
title_full Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
title_fullStr Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
title_full_unstemmed Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
title_sort Acetylcholinesterase (AChE) Activity in Embryos of Zebrafish
author Gravato, Carlos
author_facet Gravato, Carlos
Abe, Flávia Renata
de Oliveira, Danielle Palma [UNESP]
Soares, Amadeu M. V. M.
Domingues, Inês
author_role author
author2 Abe, Flávia Renata
de Oliveira, Danielle Palma [UNESP]
Soares, Amadeu M. V. M.
Domingues, Inês
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Lisboa
Universidade de São Paulo (USP)
Universidade de Aveiro
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Gravato, Carlos
Abe, Flávia Renata
de Oliveira, Danielle Palma [UNESP]
Soares, Amadeu M. V. M.
Domingues, Inês
dc.subject.por.fl_str_mv AChE
Biomarkers
Danio rerio
Ellman’s technique
Embryos
Neurotoxicity
topic AChE
Biomarkers
Danio rerio
Ellman’s technique
Embryos
Neurotoxicity
description Acetylcholinesterase (AChE) is a useful biomarker for organophosphate and carbamate pesticides exposure. The inhibition of this enzyme has been associated with neurotoxicity and alterations at higher levels of biological organization, such as behavior and development impairments. In this chapter, we describe the methodologies for analyses of AChE activity in pools of 96 h of embryos of zebrafish (Danio rerio) using a spectrophotometric method adapted to 96-well microtiter plates.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:20:50Z
2021-06-25T10:20:50Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-0716-1091-6_10
Methods in Molecular Biology, v. 2240, p. 119-124.
1940-6029
1064-3745
http://hdl.handle.net/11449/205765
10.1007/978-1-0716-1091-6_10
2-s2.0-85099678031
url http://dx.doi.org/10.1007/978-1-0716-1091-6_10
http://hdl.handle.net/11449/205765
identifier_str_mv Methods in Molecular Biology, v. 2240, p. 119-124.
1940-6029
1064-3745
10.1007/978-1-0716-1091-6_10
2-s2.0-85099678031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 119-124
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129573634703360

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