Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.flora.2019.151459 http://hdl.handle.net/11449/189575 |
Resumo: | The morphology, histochemistry and ultrastructure of glandular trichomes have been studied in several plant species and their subcellular features are closely related to the composition of the secretion produced. Recent studies have proposed a fundamental role for cytoskeletal elements in the intracellular processes of secretion, culminating with storage of the exudate in cellular compartments or its extracellular release. However, this topic has been poorly studied in plant secretory cells. Ocimum gratissimum L. (Lamiaceae) is an aromatic species widely utilized as a condiment and in popular medicine. Here, we analyzed the distribution, morphology and histochemistry of the glandular trichomes in O. gratissimum, highlighting the distribution of the microtubules and actin filaments in the different glandular morphotypes. Glandular trichomes were observed in the stem, leaves, bracts, sepals and adaxial surface of the petals. Three morphotypes of glandular trichomes were identified and found to secrete different categories of chemical compounds. Plastids devoid of grana and smooth endoplasmic reticulum were abundant in cells secreting lipophilic substances. In these cells, microtubules were intensively labeled. Cells producing mainly hydrophilic compounds were characterized by the presence of dictyosomes with vesicles sprouting. Actin filaments were strongly marked in these cells. Our convergent techniques in microscopy enabled us to establish an association among the glandular morphotypes, their subcellular aspects, the prevalence of the different cytoskeletal elements in their cells and the composition of the secretion produced by them. |
id |
UNSP_43d1fbccc4ba1ac301138b7637e62286 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/189575 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeletonActin filamentsGlandsMicrotubulesSecretionSubcellular featuresThe morphology, histochemistry and ultrastructure of glandular trichomes have been studied in several plant species and their subcellular features are closely related to the composition of the secretion produced. Recent studies have proposed a fundamental role for cytoskeletal elements in the intracellular processes of secretion, culminating with storage of the exudate in cellular compartments or its extracellular release. However, this topic has been poorly studied in plant secretory cells. Ocimum gratissimum L. (Lamiaceae) is an aromatic species widely utilized as a condiment and in popular medicine. Here, we analyzed the distribution, morphology and histochemistry of the glandular trichomes in O. gratissimum, highlighting the distribution of the microtubules and actin filaments in the different glandular morphotypes. Glandular trichomes were observed in the stem, leaves, bracts, sepals and adaxial surface of the petals. Three morphotypes of glandular trichomes were identified and found to secrete different categories of chemical compounds. Plastids devoid of grana and smooth endoplasmic reticulum were abundant in cells secreting lipophilic substances. In these cells, microtubules were intensively labeled. Cells producing mainly hydrophilic compounds were characterized by the presence of dictyosomes with vesicles sprouting. Actin filaments were strongly marked in these cells. Our convergent techniques in microscopy enabled us to establish an association among the glandular morphotypes, their subcellular aspects, the prevalence of the different cytoskeletal elements in their cells and the composition of the secretion produced by them.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual PaulistaUNESP - São Paulo State University Institute of Biosciences Department of Botany, Botucatu cityUFRRJ - Federal Rural University of Rio de Janeiro Institute of Biological and Health Sciences Department of Botany, Seropédica cityUNESP - São Paulo State University Institute of Biosciences Department of Botany, Botucatu cityUniversidade Estadual Paulista (Unesp)Institute of Biological and Health SciencesTozin, Luiz Ricardo dos Santos [UNESP]Rodrigues, Tatiane Maria [UNESP]2019-10-06T16:45:07Z2019-10-06T16:45:07Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.flora.2019.151459Flora: Morphology, Distribution, Functional Ecology of Plants, v. 259.0367-2530http://hdl.handle.net/11449/18957510.1016/j.flora.2019.1514592-s2.0-85071466455Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFlora: Morphology, Distribution, Functional Ecology of Plantsinfo:eu-repo/semantics/openAccess2021-10-23T19:49:55Zoai:repositorio.unesp.br:11449/189575Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:49:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
title |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
spellingShingle |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton Tozin, Luiz Ricardo dos Santos [UNESP] Actin filaments Glands Microtubules Secretion Subcellular features |
title_short |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
title_full |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
title_fullStr |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
title_full_unstemmed |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
title_sort |
Glandular trichomes in the tree-basil (Ocimum gratissimum L., Lamiaceae): Morphological features with emphasis on the cytoskeleton |
author |
Tozin, Luiz Ricardo dos Santos [UNESP] |
author_facet |
Tozin, Luiz Ricardo dos Santos [UNESP] Rodrigues, Tatiane Maria [UNESP] |
author_role |
author |
author2 |
Rodrigues, Tatiane Maria [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute of Biological and Health Sciences |
dc.contributor.author.fl_str_mv |
Tozin, Luiz Ricardo dos Santos [UNESP] Rodrigues, Tatiane Maria [UNESP] |
dc.subject.por.fl_str_mv |
Actin filaments Glands Microtubules Secretion Subcellular features |
topic |
Actin filaments Glands Microtubules Secretion Subcellular features |
description |
The morphology, histochemistry and ultrastructure of glandular trichomes have been studied in several plant species and their subcellular features are closely related to the composition of the secretion produced. Recent studies have proposed a fundamental role for cytoskeletal elements in the intracellular processes of secretion, culminating with storage of the exudate in cellular compartments or its extracellular release. However, this topic has been poorly studied in plant secretory cells. Ocimum gratissimum L. (Lamiaceae) is an aromatic species widely utilized as a condiment and in popular medicine. Here, we analyzed the distribution, morphology and histochemistry of the glandular trichomes in O. gratissimum, highlighting the distribution of the microtubules and actin filaments in the different glandular morphotypes. Glandular trichomes were observed in the stem, leaves, bracts, sepals and adaxial surface of the petals. Three morphotypes of glandular trichomes were identified and found to secrete different categories of chemical compounds. Plastids devoid of grana and smooth endoplasmic reticulum were abundant in cells secreting lipophilic substances. In these cells, microtubules were intensively labeled. Cells producing mainly hydrophilic compounds were characterized by the presence of dictyosomes with vesicles sprouting. Actin filaments were strongly marked in these cells. Our convergent techniques in microscopy enabled us to establish an association among the glandular morphotypes, their subcellular aspects, the prevalence of the different cytoskeletal elements in their cells and the composition of the secretion produced by them. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:45:07Z 2019-10-06T16:45:07Z 2019-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.flora.2019.151459 Flora: Morphology, Distribution, Functional Ecology of Plants, v. 259. 0367-2530 http://hdl.handle.net/11449/189575 10.1016/j.flora.2019.151459 2-s2.0-85071466455 |
url |
http://dx.doi.org/10.1016/j.flora.2019.151459 http://hdl.handle.net/11449/189575 |
identifier_str_mv |
Flora: Morphology, Distribution, Functional Ecology of Plants, v. 259. 0367-2530 10.1016/j.flora.2019.151459 2-s2.0-85071466455 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Flora: Morphology, Distribution, Functional Ecology of Plants |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964881678499840 |