Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0257992 http://hdl.handle.net/11449/229734 |
Resumo: | Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group. |
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Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complexTriatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Parasitologia Departamento de Ciências Biológicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, São PauloInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” IBB UNESP, São PauloLaboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” IBILCE/UNESP, São PauloLaboratório de Parasitologia Departamento de Ciências Biológicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, São PauloInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” IBB UNESP, São PauloLaboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” IBILCE/UNESP, São PauloUniversidade Estadual Paulista (UNESP)Pinotti, Heloisa [UNESP]de Oliveira, Jader [UNESP]Ravazi, Amanda [UNESP]Madeira, Fernanda Fernandez [UNESP]dos Reis, Yago Visinho [UNESP]de Oliveira, Ana Beatriz Bortolozo [UNESP]de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]da Rosa, João Aristeu [UNESP]Alevi, Kaio Cesar Chaboli [UNESP]2022-04-29T08:35:33Z2022-04-29T08:35:33Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0257992PLoS ONE, v. 16, n. 10 October, 2021.1932-6203http://hdl.handle.net/11449/22973410.1371/journal.pone.02579922-s2.0-85117327357Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-06-24T13:07:27Zoai:repositorio.unesp.br:11449/229734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:45:22.683002Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
title |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
spellingShingle |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex Pinotti, Heloisa [UNESP] |
title_short |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
title_full |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
title_fullStr |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
title_full_unstemmed |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
title_sort |
Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex |
author |
Pinotti, Heloisa [UNESP] |
author_facet |
Pinotti, Heloisa [UNESP] de Oliveira, Jader [UNESP] Ravazi, Amanda [UNESP] Madeira, Fernanda Fernandez [UNESP] dos Reis, Yago Visinho [UNESP] de Oliveira, Ana Beatriz Bortolozo [UNESP] de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] da Rosa, João Aristeu [UNESP] Alevi, Kaio Cesar Chaboli [UNESP] |
author_role |
author |
author2 |
de Oliveira, Jader [UNESP] Ravazi, Amanda [UNESP] Madeira, Fernanda Fernandez [UNESP] dos Reis, Yago Visinho [UNESP] de Oliveira, Ana Beatriz Bortolozo [UNESP] de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] da Rosa, João Aristeu [UNESP] Alevi, Kaio Cesar Chaboli [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Pinotti, Heloisa [UNESP] de Oliveira, Jader [UNESP] Ravazi, Amanda [UNESP] Madeira, Fernanda Fernandez [UNESP] dos Reis, Yago Visinho [UNESP] de Oliveira, Ana Beatriz Bortolozo [UNESP] de Azeredo-Oliveira, Maria Tercília Vilela [UNESP] da Rosa, João Aristeu [UNESP] Alevi, Kaio Cesar Chaboli [UNESP] |
description |
Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-01 2022-04-29T08:35:33Z 2022-04-29T08:35:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0257992 PLoS ONE, v. 16, n. 10 October, 2021. 1932-6203 http://hdl.handle.net/11449/229734 10.1371/journal.pone.0257992 2-s2.0-85117327357 |
url |
http://dx.doi.org/10.1371/journal.pone.0257992 http://hdl.handle.net/11449/229734 |
identifier_str_mv |
PLoS ONE, v. 16, n. 10 October, 2021. 1932-6203 10.1371/journal.pone.0257992 2-s2.0-85117327357 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128696413847552 |