Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex

Detalhes bibliográficos
Autor(a) principal: Pinotti, Heloisa [UNESP]
Data de Publicação: 2021
Outros Autores: de Oliveira, Jader [UNESP], Ravazi, Amanda [UNESP], Madeira, Fernanda Fernandez [UNESP], dos Reis, Yago Visinho [UNESP], de Oliveira, Ana Beatriz Bortolozo [UNESP], de Azeredo-Oliveira, Maria Tercília Vilela [UNESP], da Rosa, João Aristeu [UNESP], Alevi, Kaio Cesar Chaboli [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0257992
http://hdl.handle.net/11449/229734
Resumo: Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.
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spelling Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complexTriatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Parasitologia Departamento de Ciências Biológicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, São PauloInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” IBB UNESP, São PauloLaboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” IBILCE/UNESP, São PauloLaboratório de Parasitologia Departamento de Ciências Biológicas Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Ciências Farmacêuticas, São PauloInstituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” IBB UNESP, São PauloLaboratório de Biologia Celular Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas Universidade Estadual Paulista “Júlio de Mesquita Filho” IBILCE/UNESP, São PauloUniversidade Estadual Paulista (UNESP)Pinotti, Heloisa [UNESP]de Oliveira, Jader [UNESP]Ravazi, Amanda [UNESP]Madeira, Fernanda Fernandez [UNESP]dos Reis, Yago Visinho [UNESP]de Oliveira, Ana Beatriz Bortolozo [UNESP]de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]da Rosa, João Aristeu [UNESP]Alevi, Kaio Cesar Chaboli [UNESP]2022-04-29T08:35:33Z2022-04-29T08:35:33Z2021-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0257992PLoS ONE, v. 16, n. 10 October, 2021.1932-6203http://hdl.handle.net/11449/22973410.1371/journal.pone.02579922-s2.0-85117327357Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-06-24T13:07:27Zoai:repositorio.unesp.br:11449/229734Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:45:22.683002Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
spellingShingle Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
Pinotti, Heloisa [UNESP]
title_short Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_full Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_fullStr Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_full_unstemmed Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
title_sort Revisiting the hybridization processes in the Triatoma brasiliensis complex (Hemiptera, Triatominae): Interspecific genomic compatibility point to a possible recent diversification of the species grouped in this monophyletic complex
author Pinotti, Heloisa [UNESP]
author_facet Pinotti, Heloisa [UNESP]
de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
Madeira, Fernanda Fernandez [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
author_role author
author2 de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
Madeira, Fernanda Fernandez [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Pinotti, Heloisa [UNESP]
de Oliveira, Jader [UNESP]
Ravazi, Amanda [UNESP]
Madeira, Fernanda Fernandez [UNESP]
dos Reis, Yago Visinho [UNESP]
de Oliveira, Ana Beatriz Bortolozo [UNESP]
de Azeredo-Oliveira, Maria Tercília Vilela [UNESP]
da Rosa, João Aristeu [UNESP]
Alevi, Kaio Cesar Chaboli [UNESP]
description Triatomines are hematophagous insects of great epidemiological importance, since they are vectors of the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Triatoma brasiliensis complex is a monophyletic group formed by two subspecies and six species: T. b. brasiliensis, T. b. macromelasoma, T. bahiensis, T. juazeirensis, T. lenti, T. melanica, T. petrocchiae and T. sherlocki. The specific status of several species grouped in the T. brasiliensis complex was confirmed from experimental crossing and analysis of reproductive barriers. Thus, we perform interspecific experimental crosses between T. lenti and other species and subspecies of the T. brasiliensis complex and perform morphological analysis of the gonads and cytogenetic analysis in the homeologous chromosomes of the hybrids of first generation (F1). Besides that, we rescue all the literature data associated with the study of reproductive barriers in this monophyletic complex of species and subspecies. For all crosses performed between T. b. brasiliensis, T. b. macromelasoma, T. juazeirensis and T. melanica with T. lenti, interspecific copulas occurred (showing absence of mechanical isolation), hybrids were obtained, none of the male hybrids presented the phenomenon of gonadal dysgenesis and 100% pairing between the chromosomes homeologous of the hybrids was observed. Thus, we demonstrate that there are no pre-zygotic reproductive barriers installed between T. lenti and the species and subspecies of the T. brasiliensis complex. In addition, we demonstrate that the hybrids obtained between these crosses have high genomic compatibility and the absence of gonadal dysgenesis. These results point to reproductive compatibility between T. lenti and species and subspecies of the T. brasiliensis complex (confirming its inclusion in the complex) and lead us to suggest a possible recent diversification of the taxa of this monophyletic group.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-01
2022-04-29T08:35:33Z
2022-04-29T08:35:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0257992
PLoS ONE, v. 16, n. 10 October, 2021.
1932-6203
http://hdl.handle.net/11449/229734
10.1371/journal.pone.0257992
2-s2.0-85117327357
url http://dx.doi.org/10.1371/journal.pone.0257992
http://hdl.handle.net/11449/229734
identifier_str_mv PLoS ONE, v. 16, n. 10 October, 2021.
1932-6203
10.1371/journal.pone.0257992
2-s2.0-85117327357
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS ONE
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
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institution UNESP
reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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