Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2017/1491405 http://hdl.handle.net/11449/175103 |
Resumo: | A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes. |
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Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory GenesA high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes.Department of Morphology School of Dentistry at Araraquara São Paulo State University (UNESP)Department of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)Postgraduate Program in Sciences of the University of FrancaDepartment of Genetics Faculty of Medicine of Ribeirão Preto University of São Paulo (USP)Department of Biology Faculty of Philosophy Sciences and Letters at Ribeirão Preto University of São Paulo (USP)Department of Morphology School of Dentistry at Araraquara São Paulo State University (UNESP)Department of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Postgraduate Program in Sciences of the University of FrancaUniversidade de São Paulo (USP)Nepomuceno, Rafael [UNESP]Villela, Bárbara Scoralick [UNESP]Corbi, Sâmia Cruz Tfaile [UNESP]Bastos, Alliny De Souza [UNESP]Dos Santos, Raquel AlvesTakahashi, Catarina SatieOrrico, Silvana Regina Perez [UNESP]Scarel-Caminaga, Raquel Mantuaneli [UNESP]2018-12-11T17:14:23Z2018-12-11T17:14:23Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2017/1491405Mediators of Inflammation, v. 2017.1466-18610962-9351http://hdl.handle.net/11449/17510310.1155/2017/14914052-s2.0-850285055582-s2.0-85028505558.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators of Inflammation1,3701,370info:eu-repo/semantics/openAccess2023-11-21T06:10:17Zoai:repositorio.unesp.br:11449/175103Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-21T06:10:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
title |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
spellingShingle |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes Nepomuceno, Rafael [UNESP] |
title_short |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
title_full |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
title_fullStr |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
title_full_unstemmed |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
title_sort |
Dyslipidemia rather than Type 2 Diabetes Mellitus or Chronic Periodontitis Affects the Systemic Expression of Pro- and Anti-Inflammatory Genes |
author |
Nepomuceno, Rafael [UNESP] |
author_facet |
Nepomuceno, Rafael [UNESP] Villela, Bárbara Scoralick [UNESP] Corbi, Sâmia Cruz Tfaile [UNESP] Bastos, Alliny De Souza [UNESP] Dos Santos, Raquel Alves Takahashi, Catarina Satie Orrico, Silvana Regina Perez [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author_role |
author |
author2 |
Villela, Bárbara Scoralick [UNESP] Corbi, Sâmia Cruz Tfaile [UNESP] Bastos, Alliny De Souza [UNESP] Dos Santos, Raquel Alves Takahashi, Catarina Satie Orrico, Silvana Regina Perez [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Postgraduate Program in Sciences of the University of Franca Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nepomuceno, Rafael [UNESP] Villela, Bárbara Scoralick [UNESP] Corbi, Sâmia Cruz Tfaile [UNESP] Bastos, Alliny De Souza [UNESP] Dos Santos, Raquel Alves Takahashi, Catarina Satie Orrico, Silvana Regina Perez [UNESP] Scarel-Caminaga, Raquel Mantuaneli [UNESP] |
description |
A high percentage of type 2 diabetes mellitus (T2D) patients are also affected by dyslipidemia and chronic periodontitis (CP), but no studies have determined the gene expression in patients that are simultaneously affected by all three diseases. We investigated the systemic expression of immune-related genes in T2D, dyslipidemia, and CP patients. One hundred and fifty patients were separated into five groups containing 30 individuals each: (G1) poorly controlled T2D with dyslipidemia and CP; (G2) well-controlled T2D with dyslipidemia and CP; (G3) normoglycemic individuals with dyslipidemia and CP; (G4) healthy individuals with CP; (G5) systemic and periodontally healthy individuals. Blood analyses of lipid and glycemic profiles were carried out. The expression of genes, including IL10, JAK1, STAT3, SOCS3, IP10, ICAM1, IFNA, IFNG, STAT1, and IRF1, was investigated by RT-qPCR. Patients with dyslipidemia demonstrated statistically higher expression of the IL10 and IFNA genes, while IFNG, IP10, IRF1, JAK1, and STAT3 were lower in comparison with nondyslipidemic patients. Anti-inflammatory genes, such as IL10, positively correlated with parameters of glucose, lipid, and periodontal profiles, while proinflammatory genes, such as IFNG, were negatively correlated with these parameters. We conclude that dyslipidemia appears to be the primary disease that is associated with gene expression of immune-related genes, while parameters of T2D and CP were correlated with the expression of these important immune genes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:14:23Z 2018-12-11T17:14:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2017/1491405 Mediators of Inflammation, v. 2017. 1466-1861 0962-9351 http://hdl.handle.net/11449/175103 10.1155/2017/1491405 2-s2.0-85028505558 2-s2.0-85028505558.pdf |
url |
http://dx.doi.org/10.1155/2017/1491405 http://hdl.handle.net/11449/175103 |
identifier_str_mv |
Mediators of Inflammation, v. 2017. 1466-1861 0962-9351 10.1155/2017/1491405 2-s2.0-85028505558 2-s2.0-85028505558.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators of Inflammation 1,370 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965016102797312 |