Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype

Detalhes bibliográficos
Autor(a) principal: Garcia, Bruna G.
Data de Publicação: 2019
Outros Autores: Castro, Felipe S., Vieira, Mônica A.M., Girão, Dennys M., Uenishi, Lucas T., Cergole-Novella, Maria C., dos Santos, Luis F., Piazza, Roxane M.F., Hernandes, Rodrigo T. [UNESP], Gomes, Tânia A.T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ijmm.2018.12.001
http://hdl.handle.net/11449/188490
Resumo: Typical enteropathogenic Escherichia coli strains (tEPEC) cause attaching/effacing lesions in eukaryotic cells and produce the bundle-forming pilus (BFP), which interweaves and aggregates bacteria, resulting in the localized adherence (LA) pattern on eukaryotic cells. Previously, we identified tEPEC strains (serotype O119:H6) that exhibited LA simultaneously with an aggregative adherence (AA)-like pattern (LA/AA-like+). Remarkably, AA is characteristically produced by strains of enteroaggregative E. coli (EAEC), another diarrheagenic E. coli pathovar. In one LA/AA-like + strain (Ec404/03), we identified a conjugative plasmid containing the pil operon, which encodes the Pil fimbriae. Moreover, a pil operon associated with an AA pattern and plasmid transfer had been previously described in the EAEC C1096 strain. In this study, we investigated the occurrence of the two pilS alleles (pilSEc404 and pilSC1096) in tEPEC strains of different serotypes, origins and years of isolation. We also examined the potential relationship of pilS with the AA-like phenotype, its ability to be transferred by conjugation, and occurrence among strains of the other E. coli pathovars. The pilS alleles were found in 90 (55.2%) of 163 tEPEC strains, with pilSEc404 occurring more often (30.7%) than pilSC1096 (25.1%). About 21 tEPEC serotypes carried pilS. The pilS alleles were found in tEPEC strains from Chile, Peru and different Brazilian cities, with the oldest strain being isolated in 1966. No absolute correlation was found between the presence of pilS and the AA-like pattern. Conjugative pilS transfer was detected in 26.2% of pilSEc404+ strains and in 65.1% of pilSC1096+ strains, but only pilSEc404+ transconjugants were AA-like+, thus suggesting that the latter allele might need a different genetic background to express this phenotype. pilS was found in all other E. coli pathovars, where it was most prevalent in enterotoxigenic E. coli. More studies are needed to understand the mechanisms involved in the regulation of Pil expression and production.
id UNSP_447ff21ae157a987b048689b3b5f76a4
oai_identifier_str oai:repositorio.unesp.br:11449/188490
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotypeAggregative adherenceDiarrheagenic E. coliEnteropathogenic Escherichia coliHorizontal gene transferPil fimbriapilSTypical enteropathogenic Escherichia coli strains (tEPEC) cause attaching/effacing lesions in eukaryotic cells and produce the bundle-forming pilus (BFP), which interweaves and aggregates bacteria, resulting in the localized adherence (LA) pattern on eukaryotic cells. Previously, we identified tEPEC strains (serotype O119:H6) that exhibited LA simultaneously with an aggregative adherence (AA)-like pattern (LA/AA-like+). Remarkably, AA is characteristically produced by strains of enteroaggregative E. coli (EAEC), another diarrheagenic E. coli pathovar. In one LA/AA-like + strain (Ec404/03), we identified a conjugative plasmid containing the pil operon, which encodes the Pil fimbriae. Moreover, a pil operon associated with an AA pattern and plasmid transfer had been previously described in the EAEC C1096 strain. In this study, we investigated the occurrence of the two pilS alleles (pilSEc404 and pilSC1096) in tEPEC strains of different serotypes, origins and years of isolation. We also examined the potential relationship of pilS with the AA-like phenotype, its ability to be transferred by conjugation, and occurrence among strains of the other E. coli pathovars. The pilS alleles were found in 90 (55.2%) of 163 tEPEC strains, with pilSEc404 occurring more often (30.7%) than pilSC1096 (25.1%). About 21 tEPEC serotypes carried pilS. The pilS alleles were found in tEPEC strains from Chile, Peru and different Brazilian cities, with the oldest strain being isolated in 1966. No absolute correlation was found between the presence of pilS and the AA-like pattern. Conjugative pilS transfer was detected in 26.2% of pilSEc404+ strains and in 65.1% of pilSC1096+ strains, but only pilSEc404+ transconjugants were AA-like+, thus suggesting that the latter allele might need a different genetic background to express this phenotype. pilS was found in all other E. coli pathovars, where it was most prevalent in enterotoxigenic E. coli. More studies are needed to understand the mechanisms involved in the regulation of Pil expression and production.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Microbiologia Imunologia e Parasitologia Escola Paulista de Medicina Universidade Federal de São PauloInstituto de Microbiologia Paulo de Góes Universidade Federal do Rio de JaneiroLaboratório Regional de Santo André VIII Instituto Adolfo LutzCentro de Bacteriologia Núcleo de Doenças Entéricas Instituto Adolfo LutzLaboratório de Bacteriologia Instituto ButantanDepartamento de Microbiologia e Imunologia Universidade Estadual Paulista (UNESP) Instituto de BiociênciasDepartamento de Microbiologia e Imunologia Universidade Estadual Paulista (UNESP) Instituto de BiociênciasCNPq: #150392/2017-6FAPESP: #2015/05971-0Universidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)Instituto Adolfo LutzInstituto ButantanUniversidade Estadual Paulista (Unesp)Garcia, Bruna G.Castro, Felipe S.Vieira, Mônica A.M.Girão, Dennys M.Uenishi, Lucas T.Cergole-Novella, Maria C.dos Santos, Luis F.Piazza, Roxane M.F.Hernandes, Rodrigo T. [UNESP]Gomes, Tânia A.T.2019-10-06T16:09:56Z2019-10-06T16:09:56Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article66-72http://dx.doi.org/10.1016/j.ijmm.2018.12.001International Journal of Medical Microbiology, v. 309, n. 1, p. 66-72, 2019.1618-06071438-4221http://hdl.handle.net/11449/18849010.1016/j.ijmm.2018.12.0012-s2.0-85058403502Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Medical Microbiologyinfo:eu-repo/semantics/openAccess2021-10-22T21:16:14Zoai:repositorio.unesp.br:11449/188490Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:10:25.374096Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
title Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
spellingShingle Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
Garcia, Bruna G.
Aggregative adherence
Diarrheagenic E. coli
Enteropathogenic Escherichia coli
Horizontal gene transfer
Pil fimbria
pilS
title_short Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
title_full Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
title_fullStr Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
title_full_unstemmed Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
title_sort Distribution of the pilS gene in Escherichia coli pathovars, its transfer ability and influence in the typical enteropathogenic E. coli adherence phenotype
author Garcia, Bruna G.
author_facet Garcia, Bruna G.
Castro, Felipe S.
Vieira, Mônica A.M.
Girão, Dennys M.
Uenishi, Lucas T.
Cergole-Novella, Maria C.
dos Santos, Luis F.
Piazza, Roxane M.F.
Hernandes, Rodrigo T. [UNESP]
Gomes, Tânia A.T.
author_role author
author2 Castro, Felipe S.
Vieira, Mônica A.M.
Girão, Dennys M.
Uenishi, Lucas T.
Cergole-Novella, Maria C.
dos Santos, Luis F.
Piazza, Roxane M.F.
Hernandes, Rodrigo T. [UNESP]
Gomes, Tânia A.T.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Instituto Adolfo Lutz
Instituto Butantan
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Garcia, Bruna G.
Castro, Felipe S.
Vieira, Mônica A.M.
Girão, Dennys M.
Uenishi, Lucas T.
Cergole-Novella, Maria C.
dos Santos, Luis F.
Piazza, Roxane M.F.
Hernandes, Rodrigo T. [UNESP]
Gomes, Tânia A.T.
dc.subject.por.fl_str_mv Aggregative adherence
Diarrheagenic E. coli
Enteropathogenic Escherichia coli
Horizontal gene transfer
Pil fimbria
pilS
topic Aggregative adherence
Diarrheagenic E. coli
Enteropathogenic Escherichia coli
Horizontal gene transfer
Pil fimbria
pilS
description Typical enteropathogenic Escherichia coli strains (tEPEC) cause attaching/effacing lesions in eukaryotic cells and produce the bundle-forming pilus (BFP), which interweaves and aggregates bacteria, resulting in the localized adherence (LA) pattern on eukaryotic cells. Previously, we identified tEPEC strains (serotype O119:H6) that exhibited LA simultaneously with an aggregative adherence (AA)-like pattern (LA/AA-like+). Remarkably, AA is characteristically produced by strains of enteroaggregative E. coli (EAEC), another diarrheagenic E. coli pathovar. In one LA/AA-like + strain (Ec404/03), we identified a conjugative plasmid containing the pil operon, which encodes the Pil fimbriae. Moreover, a pil operon associated with an AA pattern and plasmid transfer had been previously described in the EAEC C1096 strain. In this study, we investigated the occurrence of the two pilS alleles (pilSEc404 and pilSC1096) in tEPEC strains of different serotypes, origins and years of isolation. We also examined the potential relationship of pilS with the AA-like phenotype, its ability to be transferred by conjugation, and occurrence among strains of the other E. coli pathovars. The pilS alleles were found in 90 (55.2%) of 163 tEPEC strains, with pilSEc404 occurring more often (30.7%) than pilSC1096 (25.1%). About 21 tEPEC serotypes carried pilS. The pilS alleles were found in tEPEC strains from Chile, Peru and different Brazilian cities, with the oldest strain being isolated in 1966. No absolute correlation was found between the presence of pilS and the AA-like pattern. Conjugative pilS transfer was detected in 26.2% of pilSEc404+ strains and in 65.1% of pilSC1096+ strains, but only pilSEc404+ transconjugants were AA-like+, thus suggesting that the latter allele might need a different genetic background to express this phenotype. pilS was found in all other E. coli pathovars, where it was most prevalent in enterotoxigenic E. coli. More studies are needed to understand the mechanisms involved in the regulation of Pil expression and production.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:09:56Z
2019-10-06T16:09:56Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ijmm.2018.12.001
International Journal of Medical Microbiology, v. 309, n. 1, p. 66-72, 2019.
1618-0607
1438-4221
http://hdl.handle.net/11449/188490
10.1016/j.ijmm.2018.12.001
2-s2.0-85058403502
url http://dx.doi.org/10.1016/j.ijmm.2018.12.001
http://hdl.handle.net/11449/188490
identifier_str_mv International Journal of Medical Microbiology, v. 309, n. 1, p. 66-72, 2019.
1618-0607
1438-4221
10.1016/j.ijmm.2018.12.001
2-s2.0-85058403502
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Medical Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 66-72
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129592494391296