Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/7323875 http://hdl.handle.net/11449/173243 |
Resumo: | The present study compared the production and the catalytic properties of amylolytic enzymes obtained from the fungi Lichtheimia ramosa (mesophilic) and Thermoascus aurantiacus (thermophilic). The highest amylase production in both fungi was observed in wheat bran supplemented with nutrient solution (pH 4.0) after 96 hours of cultivation, reaching 417.2 U/g of dry substrate (or 41.72 U/mL) and 144.5 U/g of dry substrate (or 14.45 U/mL) for L. ramosa and T. aurantiacus, respectively. The enzymes showed higher catalytic activity at pH 6.0 at 60°C. The amylases produced by L. ramosa and T. aurantiacus were stable between pH 3.5-10.5 and pH 4.5-9.5, respectively. The amylase of L. ramosa was stable at 55°C after 1 hour of incubation, whereas that of T. aurantiacus maintained 60% of its original activity under the same conditions. Both enzymes were active in the presence of ethanol. The enzymes hydrolyzed starch from different sources, with the best results obtained with corn starch. The enzymatic complex produced by L. ramosa showed dextrinizing and saccharifying potential. The enzymatic extract produced by the fungus T. aurantiacus presented only saccharifying potential, releasing glucose monomers as the main hydrolysis product. |
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Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentationThe present study compared the production and the catalytic properties of amylolytic enzymes obtained from the fungi Lichtheimia ramosa (mesophilic) and Thermoascus aurantiacus (thermophilic). The highest amylase production in both fungi was observed in wheat bran supplemented with nutrient solution (pH 4.0) after 96 hours of cultivation, reaching 417.2 U/g of dry substrate (or 41.72 U/mL) and 144.5 U/g of dry substrate (or 14.45 U/mL) for L. ramosa and T. aurantiacus, respectively. The enzymes showed higher catalytic activity at pH 6.0 at 60°C. The amylases produced by L. ramosa and T. aurantiacus were stable between pH 3.5-10.5 and pH 4.5-9.5, respectively. The amylase of L. ramosa was stable at 55°C after 1 hour of incubation, whereas that of T. aurantiacus maintained 60% of its original activity under the same conditions. Both enzymes were active in the presence of ethanol. The enzymes hydrolyzed starch from different sources, with the best results obtained with corn starch. The enzymatic complex produced by L. ramosa showed dextrinizing and saccharifying potential. The enzymatic extract produced by the fungus T. aurantiacus presented only saccharifying potential, releasing glucose monomers as the main hydrolysis product.Laboratory of Enzymology and Fermentation Processes Faculty of Biological and Environmental Sciences Federal University of Grande Dourados (FCBA/UFGD), Rodovia Dourados/Itahum, km 12Faculty of Engineering Department of Phytotechnology Food Technology and Social Economy São Paulo State University (FEIS/UNESP), Avenida Brasil, No. 56Laboratory of Fungal Ecology and Systematics Biosciences Institute Department of Biochemistry and Microbiology São Paulo State University (IB/UNESP), Avenida 24A, No. 1515Laboratory of Bioengineering Faculty of Biological and Environmental Sciences Federal University of Grande Dourados (FCBA/UFGD), Rodovia Dourados/Itahum, km 12Faculty of Engineering Department of Phytotechnology Food Technology and Social Economy São Paulo State University (FEIS/UNESP), Avenida Brasil, No. 56Laboratory of Fungal Ecology and Systematics Biosciences Institute Department of Biochemistry and Microbiology São Paulo State University (IB/UNESP), Avenida 24A, No. 1515Federal University of Grande Dourados (FCBA/UFGD)Universidade Estadual Paulista (Unesp)De Oliveira, Ana Paula AgueroSilvestre, Maria AliceGarcia, Nayara Fernanda LisboaAlves-Prado, Heloíza Ferreira [UNESP]Rodrigues, André [UNESP]Paz, Marcelo Fossa DaFonseca, Gustavo GracianoLeite, Rodrigo Simões Ribeiro2018-12-11T17:04:18Z2018-12-11T17:04:18Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/7323875Scientific World Journal, v. 2016.1537-744X2356-6140http://hdl.handle.net/11449/17324310.1155/2016/73238752-s2.0-849787059502-s2.0-84978705950.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2024-07-05T19:08:00Zoai:repositorio.unesp.br:11449/173243Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:17:05.934754Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
title |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
spellingShingle |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation De Oliveira, Ana Paula Aguero |
title_short |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
title_full |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
title_fullStr |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
title_full_unstemmed |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
title_sort |
Production and catalytic properties of amylases from Lichtheimia ramosa and Thermoascus aurantiacus by solid-state fermentation |
author |
De Oliveira, Ana Paula Aguero |
author_facet |
De Oliveira, Ana Paula Aguero Silvestre, Maria Alice Garcia, Nayara Fernanda Lisboa Alves-Prado, Heloíza Ferreira [UNESP] Rodrigues, André [UNESP] Paz, Marcelo Fossa Da Fonseca, Gustavo Graciano Leite, Rodrigo Simões Ribeiro |
author_role |
author |
author2 |
Silvestre, Maria Alice Garcia, Nayara Fernanda Lisboa Alves-Prado, Heloíza Ferreira [UNESP] Rodrigues, André [UNESP] Paz, Marcelo Fossa Da Fonseca, Gustavo Graciano Leite, Rodrigo Simões Ribeiro |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Grande Dourados (FCBA/UFGD) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
De Oliveira, Ana Paula Aguero Silvestre, Maria Alice Garcia, Nayara Fernanda Lisboa Alves-Prado, Heloíza Ferreira [UNESP] Rodrigues, André [UNESP] Paz, Marcelo Fossa Da Fonseca, Gustavo Graciano Leite, Rodrigo Simões Ribeiro |
description |
The present study compared the production and the catalytic properties of amylolytic enzymes obtained from the fungi Lichtheimia ramosa (mesophilic) and Thermoascus aurantiacus (thermophilic). The highest amylase production in both fungi was observed in wheat bran supplemented with nutrient solution (pH 4.0) after 96 hours of cultivation, reaching 417.2 U/g of dry substrate (or 41.72 U/mL) and 144.5 U/g of dry substrate (or 14.45 U/mL) for L. ramosa and T. aurantiacus, respectively. The enzymes showed higher catalytic activity at pH 6.0 at 60°C. The amylases produced by L. ramosa and T. aurantiacus were stable between pH 3.5-10.5 and pH 4.5-9.5, respectively. The amylase of L. ramosa was stable at 55°C after 1 hour of incubation, whereas that of T. aurantiacus maintained 60% of its original activity under the same conditions. Both enzymes were active in the presence of ethanol. The enzymes hydrolyzed starch from different sources, with the best results obtained with corn starch. The enzymatic complex produced by L. ramosa showed dextrinizing and saccharifying potential. The enzymatic extract produced by the fungus T. aurantiacus presented only saccharifying potential, releasing glucose monomers as the main hydrolysis product. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:04:18Z 2018-12-11T17:04:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/7323875 Scientific World Journal, v. 2016. 1537-744X 2356-6140 http://hdl.handle.net/11449/173243 10.1155/2016/7323875 2-s2.0-84978705950 2-s2.0-84978705950.pdf |
url |
http://dx.doi.org/10.1155/2016/7323875 http://hdl.handle.net/11449/173243 |
identifier_str_mv |
Scientific World Journal, v. 2016. 1537-744X 2356-6140 10.1155/2016/7323875 2-s2.0-84978705950 2-s2.0-84978705950.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific World Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128340271300608 |