Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992005000200004 http://hdl.handle.net/11449/211851 |
Resumo: | Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis. |
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Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.delta toxinPCRStaphylococcuscoagulase-negative staphylococciCoagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis.Universidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Botucatu School of MedicineUniversidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Botucatu School of MedicineCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Marconi, C. [UNESP]Cunha, M. L. R. S. [UNESP]Araújo Jr, J. P. [UNESP]Rugolo, L. M. S. S. [UNESP]2021-07-14T10:30:35Z2021-07-14T10:30:35Z2005-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article117-128application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000200004Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005.1678-9199http://hdl.handle.net/11449/21185110.1590/S1678-91992005000200004S1678-91992005000200004S1678-91992005000200004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-11-30T06:21:41Zoai:repositorio.unesp.br:11449/211851Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:12:03.982650Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
title |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
spellingShingle |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. Marconi, C. [UNESP] delta toxin PCR Staphylococcus coagulase-negative staphylococci |
title_short |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
title_full |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
title_fullStr |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
title_full_unstemmed |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
title_sort |
Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp. |
author |
Marconi, C. [UNESP] |
author_facet |
Marconi, C. [UNESP] Cunha, M. L. R. S. [UNESP] Araújo Jr, J. P. [UNESP] Rugolo, L. M. S. S. [UNESP] |
author_role |
author |
author2 |
Cunha, M. L. R. S. [UNESP] Araújo Jr, J. P. [UNESP] Rugolo, L. M. S. S. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Marconi, C. [UNESP] Cunha, M. L. R. S. [UNESP] Araújo Jr, J. P. [UNESP] Rugolo, L. M. S. S. [UNESP] |
dc.subject.por.fl_str_mv |
delta toxin PCR Staphylococcus coagulase-negative staphylococci |
topic |
delta toxin PCR Staphylococcus coagulase-negative staphylococci |
description |
Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06 2021-07-14T10:30:35Z 2021-07-14T10:30:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992005000200004 Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005. 1678-9199 http://hdl.handle.net/11449/211851 10.1590/S1678-91992005000200004 S1678-91992005000200004 S1678-91992005000200004.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992005000200004 http://hdl.handle.net/11449/211851 |
identifier_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005. 1678-9199 10.1590/S1678-91992005000200004 S1678-91992005000200004 S1678-91992005000200004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
117-128 application/pdf |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129032748793856 |