Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.

Detalhes bibliográficos
Autor(a) principal: Marconi, C. [UNESP]
Data de Publicação: 2005
Outros Autores: Cunha, M. L. R. S. [UNESP], Araújo Jr, J. P. [UNESP], Rugolo, L. M. S. S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992005000200004
http://hdl.handle.net/11449/211851
Resumo: Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis.
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spelling Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.delta toxinPCRStaphylococcuscoagulase-negative staphylococciCoagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis.Universidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Botucatu School of MedicineUniversidade Estadual Paulista, Institute of BiosciencesUniversidade Estadual Paulista, Botucatu School of MedicineCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Marconi, C. [UNESP]Cunha, M. L. R. S. [UNESP]Araújo Jr, J. P. [UNESP]Rugolo, L. M. S. S. [UNESP]2021-07-14T10:30:35Z2021-07-14T10:30:35Z2005-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article117-128application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000200004Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005.1678-9199http://hdl.handle.net/11449/21185110.1590/S1678-91992005000200004S1678-91992005000200004S1678-91992005000200004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-11-30T06:21:41Zoai:repositorio.unesp.br:11449/211851Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:12:03.982650Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
title Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
spellingShingle Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
Marconi, C. [UNESP]
delta toxin
PCR
Staphylococcus
coagulase-negative staphylococci
title_short Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
title_full Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
title_fullStr Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
title_full_unstemmed Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
title_sort Standardization of the PCR technique for the detection of delta toxin in Staphylococcus spp.
author Marconi, C. [UNESP]
author_facet Marconi, C. [UNESP]
Cunha, M. L. R. S. [UNESP]
Araújo Jr, J. P. [UNESP]
Rugolo, L. M. S. S. [UNESP]
author_role author
author2 Cunha, M. L. R. S. [UNESP]
Araújo Jr, J. P. [UNESP]
Rugolo, L. M. S. S. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Marconi, C. [UNESP]
Cunha, M. L. R. S. [UNESP]
Araújo Jr, J. P. [UNESP]
Rugolo, L. M. S. S. [UNESP]
dc.subject.por.fl_str_mv delta toxin
PCR
Staphylococcus
coagulase-negative staphylococci
topic delta toxin
PCR
Staphylococcus
coagulase-negative staphylococci
description Coagulase-negative staphylococci (CNS), components of the normal flora of neonates, have emerged as important opportunistic pathogens of nosocomial infections that occur in neonatal intensive care units. Some authors have reported the ability of some CNS strains, particularly Staphylococcus epidermidis, to produce a toxin similar to S. aureus delta toxin. This toxin is an exoprotein that has a detergent action on the membranes of various cell types resulting in rapid cell lysis. The objectives of the present study were to standardize the Polymerase Chain Reaction (PCR) technique for the detection of the gene responsible for the production of delta toxin (hld gene) in staphylococcal species isolated from catheters and blood cultures obtained from neonates, and to compare the results to those obtained with the phenotypic synergistic hemolysis method. Detection of delta toxin by the phenotypic and genotypic method yielded similar results for the S. aureus isolates. However, in S. epidermidis, a higher positivity was observed for PCR (97.4%) compared to the synergistic hemolysis method (86.8%). Among CNS, S. epidermidis was the most frequent isolate and was a delta toxin producer. Staphylococcus simulans and S. warneri tested positive by the phenotypic method, but their positivity was not confirmed by PCR for the hld gene detection. These results indicate that different genes might be responsible for the production of this toxin in different CNS species, requiring highly specific primers for their detection. PCR was found to be a rapid and reliable method for the detection of the hld gene in S. aureus and S. epidermidis.
publishDate 2005
dc.date.none.fl_str_mv 2005-06
2021-07-14T10:30:35Z
2021-07-14T10:30:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992005000200004
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005.
1678-9199
http://hdl.handle.net/11449/211851
10.1590/S1678-91992005000200004
S1678-91992005000200004
S1678-91992005000200004.pdf
url http://dx.doi.org/10.1590/S1678-91992005000200004
http://hdl.handle.net/11449/211851
identifier_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 2, p. 117-128, 2005.
1678-9199
10.1590/S1678-91992005000200004
S1678-91992005000200004
S1678-91992005000200004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 117-128
application/pdf
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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