Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization

Detalhes bibliográficos
Autor(a) principal: Rego, Letícia do Nascimento Andrade de Almeida [UNESP]
Data de Publicação: 2013
Outros Autores: Silistino-Souza, Rosana [UNESP], Oliveira, Maria Tercília Vilela de Azeredo [UNESP], Madi-Ravazzi, Lilian [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572013000100008
http://hdl.handle.net/11449/74846
Resumo: Zaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old) compared to Z. sepsoides males, which showed a higher frequency of cells at the early stages of spermatogenesis. The head of the sperm was strongly marked by silver staining, lacto-acetic orcein and the Feulgen reaction; the P.A.S. reaction revealed glycogen granules in the testes of both species. Both species presented similar arrangement of microtubules (9+9+2), two mitochondrial derivatives of different size and 64 spermatozoa per bundle. Such similarity within the genus Zaprionus with other species of Drosophila, indicates that these structures are conserved in the family Drosophilidae. The differences observed the number and frequency of sperm cells in the early stages of spermatogenesis, between the young males of Z. indianus and Z. sepsoides, are features that may interfere with reproductive success and be related to the invasive potential of Z. indianus. © 2013, Sociedade Brasileira de Genética. Printed in Brazil.
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spelling Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterizationCytochemistryMeiosisSpermatogenesisUltrastructureZaprionus indianusZaprionus sepsoidesglycogenanimal cellanimal tissuecontrolled studycytochemistrydiploidyDrosophilidaemalemicrotubulemorphologynonhumanreproductive successspecies differentiationspermatocytespermatogenesisspermatozoonspermatozoon headstainingtestisultrastructureZaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old) compared to Z. sepsoides males, which showed a higher frequency of cells at the early stages of spermatogenesis. The head of the sperm was strongly marked by silver staining, lacto-acetic orcein and the Feulgen reaction; the P.A.S. reaction revealed glycogen granules in the testes of both species. Both species presented similar arrangement of microtubules (9+9+2), two mitochondrial derivatives of different size and 64 spermatozoa per bundle. Such similarity within the genus Zaprionus with other species of Drosophila, indicates that these structures are conserved in the family Drosophilidae. The differences observed the number and frequency of sperm cells in the early stages of spermatogenesis, between the young males of Z. indianus and Z. sepsoides, are features that may interfere with reproductive success and be related to the invasive potential of Z. indianus. © 2013, Sociedade Brasileira de Genética. Printed in Brazil.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Biologia Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista 'Julio Mesquita Filho', São José do Rio Preto, SPDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas, Universidade Estadual Paulista 'Julio Mesquita Filho', São José do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Rego, Letícia do Nascimento Andrade de Almeida [UNESP]Silistino-Souza, Rosana [UNESP]Oliveira, Maria Tercília Vilela de Azeredo [UNESP]Madi-Ravazzi, Lilian [UNESP]2014-05-27T11:28:40Z2014-05-27T11:28:40Z2013-03-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article50-60application/pdfhttp://dx.doi.org/10.1590/S1415-47572013000100008Genetics and Molecular Biology, v. 36, n. 1, p. 50-60, 2013.1415-47571678-4685http://hdl.handle.net/11449/7484610.1590/S1415-47572013000100008S1415-47572013000100008WOS:0003156521000082-s2.0-848748213132-s2.0-84874821313.pdf6045437265946340Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-27T06:12:17Zoai:repositorio.unesp.br:11449/74846Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:11:42.718536Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
title Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
spellingShingle Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
Rego, Letícia do Nascimento Andrade de Almeida [UNESP]
Cytochemistry
Meiosis
Spermatogenesis
Ultrastructure
Zaprionus indianus
Zaprionus sepsoides
glycogen
animal cell
animal tissue
controlled study
cytochemistry
diploidy
Drosophilidae
male
microtubule
morphology
nonhuman
reproductive success
species differentiation
spermatocyte
spermatogenesis
spermatozoon
spermatozoon head
staining
testis
ultrastructure
title_short Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
title_full Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
title_fullStr Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
title_full_unstemmed Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
title_sort Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): Cytochemical, structural and ultrastructural characterization
author Rego, Letícia do Nascimento Andrade de Almeida [UNESP]
author_facet Rego, Letícia do Nascimento Andrade de Almeida [UNESP]
Silistino-Souza, Rosana [UNESP]
Oliveira, Maria Tercília Vilela de Azeredo [UNESP]
Madi-Ravazzi, Lilian [UNESP]
author_role author
author2 Silistino-Souza, Rosana [UNESP]
Oliveira, Maria Tercília Vilela de Azeredo [UNESP]
Madi-Ravazzi, Lilian [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rego, Letícia do Nascimento Andrade de Almeida [UNESP]
Silistino-Souza, Rosana [UNESP]
Oliveira, Maria Tercília Vilela de Azeredo [UNESP]
Madi-Ravazzi, Lilian [UNESP]
dc.subject.por.fl_str_mv Cytochemistry
Meiosis
Spermatogenesis
Ultrastructure
Zaprionus indianus
Zaprionus sepsoides
glycogen
animal cell
animal tissue
controlled study
cytochemistry
diploidy
Drosophilidae
male
microtubule
morphology
nonhuman
reproductive success
species differentiation
spermatocyte
spermatogenesis
spermatozoon
spermatozoon head
staining
testis
ultrastructure
topic Cytochemistry
Meiosis
Spermatogenesis
Ultrastructure
Zaprionus indianus
Zaprionus sepsoides
glycogen
animal cell
animal tissue
controlled study
cytochemistry
diploidy
Drosophilidae
male
microtubule
morphology
nonhuman
reproductive success
species differentiation
spermatocyte
spermatogenesis
spermatozoon
spermatozoon head
staining
testis
ultrastructure
description Zaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old) compared to Z. sepsoides males, which showed a higher frequency of cells at the early stages of spermatogenesis. The head of the sperm was strongly marked by silver staining, lacto-acetic orcein and the Feulgen reaction; the P.A.S. reaction revealed glycogen granules in the testes of both species. Both species presented similar arrangement of microtubules (9+9+2), two mitochondrial derivatives of different size and 64 spermatozoa per bundle. Such similarity within the genus Zaprionus with other species of Drosophila, indicates that these structures are conserved in the family Drosophilidae. The differences observed the number and frequency of sperm cells in the early stages of spermatogenesis, between the young males of Z. indianus and Z. sepsoides, are features that may interfere with reproductive success and be related to the invasive potential of Z. indianus. © 2013, Sociedade Brasileira de Genética. Printed in Brazil.
publishDate 2013
dc.date.none.fl_str_mv 2013-03-18
2014-05-27T11:28:40Z
2014-05-27T11:28:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572013000100008
Genetics and Molecular Biology, v. 36, n. 1, p. 50-60, 2013.
1415-4757
1678-4685
http://hdl.handle.net/11449/74846
10.1590/S1415-47572013000100008
S1415-47572013000100008
WOS:000315652100008
2-s2.0-84874821313
2-s2.0-84874821313.pdf
6045437265946340
url http://dx.doi.org/10.1590/S1415-47572013000100008
http://hdl.handle.net/11449/74846
identifier_str_mv Genetics and Molecular Biology, v. 36, n. 1, p. 50-60, 2013.
1415-4757
1678-4685
10.1590/S1415-47572013000100008
S1415-47572013000100008
WOS:000315652100008
2-s2.0-84874821313
2-s2.0-84874821313.pdf
6045437265946340
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 50-60
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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