The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jplph.2020.153243 http://hdl.handle.net/11449/200851 |
Resumo: | The low-auxin-sensitivity tomato mutant, dgt, despite displaying reduced plant growth, has been linked to greater resistance to N deficiency. This led us to test the role of auxin resistance of dgt in NH4+ toxicity and N deficiency, compared to wild type tomato (cv. Micro-Tom, MT), grown in hydroponic media. A completely randomized design with three replications in a 2 × 4 factorial scheme was adopted, corresponding to the two tomato genotypes (MT and dgt), involving four nutritional treatments: NO3− (5 mM); NH4+ (5 mM); NO3− (5 mM) plus exogenous auxin (10 μM IAA); and N omission. The results show that NH4+ was toxic to MT but not to dgt. Under N deficiency, MT displayed a lower shoot NO3− content, a lower photosynthetic rate, and a decrease in both shoot and root dry weight. However, in dgt, no difference was observed in shoot NO3− content and photosynthetic rate between plants grown on NO3− or under N deficiency. In addition, dgt showed an increase in shoot dry weight under N deficiency. We highlight the role of auxin resistance in the adaptation of plants to NH4+ toxicity and N deficiency. |
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The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiencyAmmonium toxicityIndoleacetic acidLow nitrogenNitrogen limitation adaptationThe low-auxin-sensitivity tomato mutant, dgt, despite displaying reduced plant growth, has been linked to greater resistance to N deficiency. This led us to test the role of auxin resistance of dgt in NH4+ toxicity and N deficiency, compared to wild type tomato (cv. Micro-Tom, MT), grown in hydroponic media. A completely randomized design with three replications in a 2 × 4 factorial scheme was adopted, corresponding to the two tomato genotypes (MT and dgt), involving four nutritional treatments: NO3− (5 mM); NH4+ (5 mM); NO3− (5 mM) plus exogenous auxin (10 μM IAA); and N omission. The results show that NH4+ was toxic to MT but not to dgt. Under N deficiency, MT displayed a lower shoot NO3− content, a lower photosynthetic rate, and a decrease in both shoot and root dry weight. However, in dgt, no difference was observed in shoot NO3− content and photosynthetic rate between plants grown on NO3− or under N deficiency. In addition, dgt showed an increase in shoot dry weight under N deficiency. We highlight the role of auxin resistance in the adaptation of plants to NH4+ toxicity and N deficiency.Department of Soils and Fertilizers São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Jaboticabal Via de Acesso Prof. Paulo Donato Castelane, S/N, Vila IndustrialDepartment Ciencias del Medio Natural Universidad Publica de NavarraInstituto de Agrobiotecnologia UPNA-CSICDepartment of Biology Applied to Agriculture São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences Jaboticabal Via de Acesso Prof. Paulo Donato Castelane, S/NDepartment of Soils and Fertilizers São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Jaboticabal Via de Acesso Prof. Paulo Donato Castelane, S/N, Vila IndustrialDepartment of Biology Applied to Agriculture São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences Jaboticabal Via de Acesso Prof. Paulo Donato Castelane, S/NUniversidade Estadual Paulista (Unesp)Universidad Publica de NavarraUPNA-CSICSantos, Luiz Cláudio Nascimento [UNESP]Barreto, Rafael Ferreira [UNESP]Prado, Renato Mello [UNESP]Silva, Gilmara Pereira [UNESP]Santos, Janaína Maria RodriguesLasa, BertaAparicio-Tejo, Pedro MariaMichelena, Iker AranjueloCarvalho, Rogério Falleiros [UNESP]2020-12-12T02:17:47Z2020-12-12T02:17:47Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jplph.2020.153243Journal of Plant Physiology, v. 252.0176-1617http://hdl.handle.net/11449/20085110.1016/j.jplph.2020.1532432-s2.0-8508900448441253447531004540000-0003-1270-7372Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Physiologyinfo:eu-repo/semantics/openAccess2024-06-07T14:23:27Zoai:repositorio.unesp.br:11449/200851Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:04:39.435217Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
title |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
spellingShingle |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency Santos, Luiz Cláudio Nascimento [UNESP] Ammonium toxicity Indoleacetic acid Low nitrogen Nitrogen limitation adaptation |
title_short |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
title_full |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
title_fullStr |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
title_full_unstemmed |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
title_sort |
The auxin-resistant dgt tomato mutant grows less than the wild type but is less sensitive to ammonium toxicity and nitrogen deficiency |
author |
Santos, Luiz Cláudio Nascimento [UNESP] |
author_facet |
Santos, Luiz Cláudio Nascimento [UNESP] Barreto, Rafael Ferreira [UNESP] Prado, Renato Mello [UNESP] Silva, Gilmara Pereira [UNESP] Santos, Janaína Maria Rodrigues Lasa, Berta Aparicio-Tejo, Pedro Maria Michelena, Iker Aranjuelo Carvalho, Rogério Falleiros [UNESP] |
author_role |
author |
author2 |
Barreto, Rafael Ferreira [UNESP] Prado, Renato Mello [UNESP] Silva, Gilmara Pereira [UNESP] Santos, Janaína Maria Rodrigues Lasa, Berta Aparicio-Tejo, Pedro Maria Michelena, Iker Aranjuelo Carvalho, Rogério Falleiros [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad Publica de Navarra UPNA-CSIC |
dc.contributor.author.fl_str_mv |
Santos, Luiz Cláudio Nascimento [UNESP] Barreto, Rafael Ferreira [UNESP] Prado, Renato Mello [UNESP] Silva, Gilmara Pereira [UNESP] Santos, Janaína Maria Rodrigues Lasa, Berta Aparicio-Tejo, Pedro Maria Michelena, Iker Aranjuelo Carvalho, Rogério Falleiros [UNESP] |
dc.subject.por.fl_str_mv |
Ammonium toxicity Indoleacetic acid Low nitrogen Nitrogen limitation adaptation |
topic |
Ammonium toxicity Indoleacetic acid Low nitrogen Nitrogen limitation adaptation |
description |
The low-auxin-sensitivity tomato mutant, dgt, despite displaying reduced plant growth, has been linked to greater resistance to N deficiency. This led us to test the role of auxin resistance of dgt in NH4+ toxicity and N deficiency, compared to wild type tomato (cv. Micro-Tom, MT), grown in hydroponic media. A completely randomized design with three replications in a 2 × 4 factorial scheme was adopted, corresponding to the two tomato genotypes (MT and dgt), involving four nutritional treatments: NO3− (5 mM); NH4+ (5 mM); NO3− (5 mM) plus exogenous auxin (10 μM IAA); and N omission. The results show that NH4+ was toxic to MT but not to dgt. Under N deficiency, MT displayed a lower shoot NO3− content, a lower photosynthetic rate, and a decrease in both shoot and root dry weight. However, in dgt, no difference was observed in shoot NO3− content and photosynthetic rate between plants grown on NO3− or under N deficiency. In addition, dgt showed an increase in shoot dry weight under N deficiency. We highlight the role of auxin resistance in the adaptation of plants to NH4+ toxicity and N deficiency. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:17:47Z 2020-12-12T02:17:47Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jplph.2020.153243 Journal of Plant Physiology, v. 252. 0176-1617 http://hdl.handle.net/11449/200851 10.1016/j.jplph.2020.153243 2-s2.0-85089004484 4125344753100454 0000-0003-1270-7372 |
url |
http://dx.doi.org/10.1016/j.jplph.2020.153243 http://hdl.handle.net/11449/200851 |
identifier_str_mv |
Journal of Plant Physiology, v. 252. 0176-1617 10.1016/j.jplph.2020.153243 2-s2.0-85089004484 4125344753100454 0000-0003-1270-7372 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128752015638528 |