Determination of isotretinoin (13-cis-retinoic acid) using a sensor based on reduced graphene oxide modified with copper nanoparticles

• Autor(a) principal: Fernandes, Victoria Quintas [UNESP]
• Data de Publicação: 2020
• Outros Autores: Silva, Martin K. Leme [UNESP], Cesarino, Ivana [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.jelechem.2019.113692; http://hdl.handle.net/11449/198255
• topic: 13-cis-Retinoic acid; Copper nanoparticles; Graphene; Isotretinoin

The electrochemical behaviour of isotretinoin (13-cis-RA) on an electrode modified with a composite based on reduced graphene oxide (rGO) modified with copper nanoparticles (CuNPs) was investigated. The rGO-CuNPs composite was successfully synthesized by a simple chemical reduction step and then characterized by microscopic and electrochemical techniques. The rGO-CuNPs modified glassy carbon (GC) electrode was applied successfully in the direct electrochemical detection of isotretinoin in a 0.2 mol L− 1 phosphate buffer solution (PBS - pH 7.0) solution (as the electrolyte). Differential pulse voltammetry (DPV) was the methodology used. The presented method displayed linearity in the concentration range from 0.2 to 3.0 μmol L− 1 with a limit of detection (LOD) of 15.3 nmol L− 1. This is the lowest LOD for isotretinoin reported in the literature using an electroanalytical approach. The proposed sensor was applied in the determination of isotretinoin in synthetic plasmatic serum with a recovery of 91% from the original signal.