Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis

Detalhes bibliográficos
Autor(a) principal: dos Santos, Jéssica Diane [UNESP]
Data de Publicação: 2020
Outros Autores: Fugisaki, Luciana Ruano de Oliveira [UNESP], Medina, Rebeca Previate [UNESP], Scorzoni, Liliana [UNESP], Alves, Mariana de Sá [UNESP], de Barros, Patrícia Pimentel [UNESP], Ribeiro, Felipe Camargo [UNESP], Fuchs, Beth Burgwyn, Mylonakis, Eleftherios, Silva, Dulce Helena Siqueira [UNESP], Junqueira, Juliana Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fmicb.2020.01605
http://hdl.handle.net/11449/200826
Resumo: In the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis.
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spelling Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral CandidiasisbiofilmCandida albicansfilamentationoral candidiasisStreptococcus mutansIn the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Division of Infectious Diseases Rhode Island Hospital Warren Alpert Medical School of Brown UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Warren Alpert Medical School of Brown Universitydos Santos, Jéssica Diane [UNESP]Fugisaki, Luciana Ruano de Oliveira [UNESP]Medina, Rebeca Previate [UNESP]Scorzoni, Liliana [UNESP]Alves, Mariana de Sá [UNESP]de Barros, Patrícia Pimentel [UNESP]Ribeiro, Felipe Camargo [UNESP]Fuchs, Beth BurgwynMylonakis, EleftheriosSilva, Dulce Helena Siqueira [UNESP]Junqueira, Juliana Campos [UNESP]2020-12-12T02:17:06Z2020-12-12T02:17:06Z2020-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2020.01605Frontiers in Microbiology, v. 11.1664-302Xhttp://hdl.handle.net/11449/20082610.3389/fmicb.2020.016052-s2.0-85088788900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T15:17:15Zoai:repositorio.unesp.br:11449/200826Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:18.555435Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
title Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
spellingShingle Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
dos Santos, Jéssica Diane [UNESP]
biofilm
Candida albicans
filamentation
oral candidiasis
Streptococcus mutans
title_short Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
title_full Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
title_fullStr Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
title_full_unstemmed Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
title_sort Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
author dos Santos, Jéssica Diane [UNESP]
author_facet dos Santos, Jéssica Diane [UNESP]
Fugisaki, Luciana Ruano de Oliveira [UNESP]
Medina, Rebeca Previate [UNESP]
Scorzoni, Liliana [UNESP]
Alves, Mariana de Sá [UNESP]
de Barros, Patrícia Pimentel [UNESP]
Ribeiro, Felipe Camargo [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Silva, Dulce Helena Siqueira [UNESP]
Junqueira, Juliana Campos [UNESP]
author_role author
author2 Fugisaki, Luciana Ruano de Oliveira [UNESP]
Medina, Rebeca Previate [UNESP]
Scorzoni, Liliana [UNESP]
Alves, Mariana de Sá [UNESP]
de Barros, Patrícia Pimentel [UNESP]
Ribeiro, Felipe Camargo [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Silva, Dulce Helena Siqueira [UNESP]
Junqueira, Juliana Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Warren Alpert Medical School of Brown University
dc.contributor.author.fl_str_mv dos Santos, Jéssica Diane [UNESP]
Fugisaki, Luciana Ruano de Oliveira [UNESP]
Medina, Rebeca Previate [UNESP]
Scorzoni, Liliana [UNESP]
Alves, Mariana de Sá [UNESP]
de Barros, Patrícia Pimentel [UNESP]
Ribeiro, Felipe Camargo [UNESP]
Fuchs, Beth Burgwyn
Mylonakis, Eleftherios
Silva, Dulce Helena Siqueira [UNESP]
Junqueira, Juliana Campos [UNESP]
dc.subject.por.fl_str_mv biofilm
Candida albicans
filamentation
oral candidiasis
Streptococcus mutans
topic biofilm
Candida albicans
filamentation
oral candidiasis
Streptococcus mutans
description In the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:17:06Z
2020-12-12T02:17:06Z
2020-07-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fmicb.2020.01605
Frontiers in Microbiology, v. 11.
1664-302X
http://hdl.handle.net/11449/200826
10.3389/fmicb.2020.01605
2-s2.0-85088788900
url http://dx.doi.org/10.3389/fmicb.2020.01605
http://hdl.handle.net/11449/200826
identifier_str_mv Frontiers in Microbiology, v. 11.
1664-302X
10.3389/fmicb.2020.01605
2-s2.0-85088788900
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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