Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2020.01605 http://hdl.handle.net/11449/200826 |
Resumo: | In the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis. |
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Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral CandidiasisbiofilmCandida albicansfilamentationoral candidiasisStreptococcus mutansIn the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis.Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Division of Infectious Diseases Rhode Island Hospital Warren Alpert Medical School of Brown UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Warren Alpert Medical School of Brown Universitydos Santos, Jéssica Diane [UNESP]Fugisaki, Luciana Ruano de Oliveira [UNESP]Medina, Rebeca Previate [UNESP]Scorzoni, Liliana [UNESP]Alves, Mariana de Sá [UNESP]de Barros, Patrícia Pimentel [UNESP]Ribeiro, Felipe Camargo [UNESP]Fuchs, Beth BurgwynMylonakis, EleftheriosSilva, Dulce Helena Siqueira [UNESP]Junqueira, Juliana Campos [UNESP]2020-12-12T02:17:06Z2020-12-12T02:17:06Z2020-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2020.01605Frontiers in Microbiology, v. 11.1664-302Xhttp://hdl.handle.net/11449/20082610.3389/fmicb.2020.016052-s2.0-85088788900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T15:17:15Zoai:repositorio.unesp.br:11449/200826Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:23:18.555435Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
title |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
spellingShingle |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis dos Santos, Jéssica Diane [UNESP] biofilm Candida albicans filamentation oral candidiasis Streptococcus mutans |
title_short |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
title_full |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
title_fullStr |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
title_full_unstemmed |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
title_sort |
Streptococcus mutans Secreted Products Inhibit Candida albicans Induced Oral Candidiasis |
author |
dos Santos, Jéssica Diane [UNESP] |
author_facet |
dos Santos, Jéssica Diane [UNESP] Fugisaki, Luciana Ruano de Oliveira [UNESP] Medina, Rebeca Previate [UNESP] Scorzoni, Liliana [UNESP] Alves, Mariana de Sá [UNESP] de Barros, Patrícia Pimentel [UNESP] Ribeiro, Felipe Camargo [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Silva, Dulce Helena Siqueira [UNESP] Junqueira, Juliana Campos [UNESP] |
author_role |
author |
author2 |
Fugisaki, Luciana Ruano de Oliveira [UNESP] Medina, Rebeca Previate [UNESP] Scorzoni, Liliana [UNESP] Alves, Mariana de Sá [UNESP] de Barros, Patrícia Pimentel [UNESP] Ribeiro, Felipe Camargo [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Silva, Dulce Helena Siqueira [UNESP] Junqueira, Juliana Campos [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Warren Alpert Medical School of Brown University |
dc.contributor.author.fl_str_mv |
dos Santos, Jéssica Diane [UNESP] Fugisaki, Luciana Ruano de Oliveira [UNESP] Medina, Rebeca Previate [UNESP] Scorzoni, Liliana [UNESP] Alves, Mariana de Sá [UNESP] de Barros, Patrícia Pimentel [UNESP] Ribeiro, Felipe Camargo [UNESP] Fuchs, Beth Burgwyn Mylonakis, Eleftherios Silva, Dulce Helena Siqueira [UNESP] Junqueira, Juliana Campos [UNESP] |
dc.subject.por.fl_str_mv |
biofilm Candida albicans filamentation oral candidiasis Streptococcus mutans |
topic |
biofilm Candida albicans filamentation oral candidiasis Streptococcus mutans |
description |
In the oral cavity, Candida species form mixed biofilms with Streptococcus mutans, a pathogenic bacterium that can secrete quorum sensing molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of S. mutans, seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by Candida albicans. Active S. mutans UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve S. mutans fraction 1 (SM-F1) and fraction 2 (SM-F2). We found anti-biofilm activity for both SM-F1 and SM-F2 in a dose dependent manner and fungal growth was reduced by 2.59 and 5.98 log for SM-F1 and SM-F2, respectively. The SM-F1 and SM-F2 fractions were also capable of reducing C. albicans filamentation, however statistically significant differences were only observed for the SM-F2 (p = 0.004). SM-F2 efficacy to inhibit C. albicans was confirmed by its capacity to downregulate filamentation genes CPH1, EFG1, HWP1, and UME6. Using Galleria mellonella as an invertebrate infection model, therapeutic treatment with SM-F2 prolonged larvae survival. Examination of the antifungal capacity was extended to a murine model of oral candidiasis that exhibited a reduction in C. albicans colonization (CFU/mL) in the oral cavity when treated with SM-F1 (2.46 log) and SM-F2 (2.34 log) compared to the control (3.25 log). Although both SM-F1 and SM-F2 fractions decreased candidiasis in mice, only SM-F2 exhibited significant quantitative differences compared to the non-treated group for macroscopic lesions, hyphae invasion, tissue lesions, and inflammatory infiltrate. Taken together, these results indicate that the SM-F2 fraction contains antifungal components, providing a promising resource in the discovery of new inhibitors for oral candidiasis. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:17:06Z 2020-12-12T02:17:06Z 2020-07-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2020.01605 Frontiers in Microbiology, v. 11. 1664-302X http://hdl.handle.net/11449/200826 10.3389/fmicb.2020.01605 2-s2.0-85088788900 |
url |
http://dx.doi.org/10.3389/fmicb.2020.01605 http://hdl.handle.net/11449/200826 |
identifier_str_mv |
Frontiers in Microbiology, v. 11. 1664-302X 10.3389/fmicb.2020.01605 2-s2.0-85088788900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129514598825984 |