Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1636/CSh07-132.1 http://hdl.handle.net/11449/19947 |
Resumo: | Pholcidae is the most diverse family among haplogyne spiders but only 15 species have been analyzed cytogenetically. These studies revealed that the diploid number varies from 2n = 15 to 2n = 32, that there are three types of sex chromosome systems in males (X, X(1)X(2) and X(1)X(2)Y), and that the chromosomes are predominantly biarmed. Within the genus Mesabolivar, only Mesabolivar luteus (Keyserling 1891) has bee karyotyped, and it showed 2n = 15 = 14 + X, with all chromosomes being metacentric. In the present work, we characterize the mitotic and meiotic chromosomes of Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891). Male mitotic metaphases of the two species showed the diploid number 2n = 17 = 16 + X; oogonial cells of M. brasiliensis showed 2n = 18 = 16 + XX. In bot species, the chromosomes were exclusively biarmed, and the X chromosome was the largest element of the karyotype. Diplotene spermatocytes of the two species exhibited 811 + X and the occurrence of only one terminal or interstitial chiasma per bivalent. In M. cyancotaeniaus, metaphases II with n = 9 = 8 + X and n = 8 were found, indicating the regular segregation of all chromosomes during meiosis I. Mitotic metaphases of M. brasiliensis stained with CMA(3)/DA/DAPI revealed GC-rich chromatin in the terminal region of almost all autosomes, especially in pair 2. An earlier revision of the New World pholeids grouped M. brasiliensis and M. cyaneotaeniatus in a "southern group" and placed M. luteus in a "miscellaneous group." A molecular study showed a closer relationship between M. brasiliensis and M. cyaneotaeniatus than between M. luteus and either of these two species. The 2n = 17 found in M. brasiliensis and M. cyaneotaeniatus corroborates this hypothesis, given that M. luteus has a diploid number of 2n = 15. |
id |
UNSP_45dfac6ce1506ff2eac3ceaa95adc1c3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/19947 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analysesChromosomeHaplogynaemeiosissex chromosome systemspiderPholcidae is the most diverse family among haplogyne spiders but only 15 species have been analyzed cytogenetically. These studies revealed that the diploid number varies from 2n = 15 to 2n = 32, that there are three types of sex chromosome systems in males (X, X(1)X(2) and X(1)X(2)Y), and that the chromosomes are predominantly biarmed. Within the genus Mesabolivar, only Mesabolivar luteus (Keyserling 1891) has bee karyotyped, and it showed 2n = 15 = 14 + X, with all chromosomes being metacentric. In the present work, we characterize the mitotic and meiotic chromosomes of Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891). Male mitotic metaphases of the two species showed the diploid number 2n = 17 = 16 + X; oogonial cells of M. brasiliensis showed 2n = 18 = 16 + XX. In bot species, the chromosomes were exclusively biarmed, and the X chromosome was the largest element of the karyotype. Diplotene spermatocytes of the two species exhibited 811 + X and the occurrence of only one terminal or interstitial chiasma per bivalent. In M. cyancotaeniaus, metaphases II with n = 9 = 8 + X and n = 8 were found, indicating the regular segregation of all chromosomes during meiosis I. Mitotic metaphases of M. brasiliensis stained with CMA(3)/DA/DAPI revealed GC-rich chromatin in the terminal region of almost all autosomes, especially in pair 2. An earlier revision of the New World pholeids grouped M. brasiliensis and M. cyaneotaeniatus in a "southern group" and placed M. luteus in a "miscellaneous group." A molecular study showed a closer relationship between M. brasiliensis and M. cyaneotaeniatus than between M. luteus and either of these two species. The 2n = 17 found in M. brasiliensis and M. cyaneotaeniatus corroborates this hypothesis, given that M. luteus has a diploid number of 2n = 15.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Inst Biociencias, Dept Biol, UNESP, BR-13506900 São Paulo, BrazilUniv Estadual Mato Grosso do Sul, UEMS, Unidade Univ Mundo Novo, BR-79980000 Mundo Novo, MS, BrazilInst Butantan, Lab Artropodes, BR-05503900 São Paulo, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, UNESP, BR-13506900 São Paulo, BrazilFAPESP: 06/53275-3CNPq: 301776/2004-0Amer Arachnological SocUniversidade Estadual Paulista (Unesp)Univ Estadual Mato Grosso do SulInstituto ButantanRamlho, Manuela Oliveira [UNESP]Araujo, DouglasSchneider, Marielle Cristina [UNESP]Brescovit, Antonio DomingosCella, Doralice Maria [UNESP]2014-05-20T13:55:42Z2014-05-20T13:55:42Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article453-456http://dx.doi.org/10.1636/CSh07-132.1Journal of Arachnology. College Park: Amer Arachnological Soc, v. 36, n. 2, p. 453-456, 2008.0161-8202http://hdl.handle.net/11449/1994710.1636/CSh07-132.1WOS:000260696100028Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Arachnology1.2360,557info:eu-repo/semantics/openAccess2021-10-22T18:56:50Zoai:repositorio.unesp.br:11449/19947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:56:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
title |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
spellingShingle |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses Ramlho, Manuela Oliveira [UNESP] Chromosome Haplogynae meiosis sex chromosome system spider |
title_short |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
title_full |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
title_fullStr |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
title_full_unstemmed |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
title_sort |
Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891) (Araneomorphae, Pholcidae): close relationship reinforced by cytogenetic analyses |
author |
Ramlho, Manuela Oliveira [UNESP] |
author_facet |
Ramlho, Manuela Oliveira [UNESP] Araujo, Douglas Schneider, Marielle Cristina [UNESP] Brescovit, Antonio Domingos Cella, Doralice Maria [UNESP] |
author_role |
author |
author2 |
Araujo, Douglas Schneider, Marielle Cristina [UNESP] Brescovit, Antonio Domingos Cella, Doralice Maria [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Estadual Mato Grosso do Sul Instituto Butantan |
dc.contributor.author.fl_str_mv |
Ramlho, Manuela Oliveira [UNESP] Araujo, Douglas Schneider, Marielle Cristina [UNESP] Brescovit, Antonio Domingos Cella, Doralice Maria [UNESP] |
dc.subject.por.fl_str_mv |
Chromosome Haplogynae meiosis sex chromosome system spider |
topic |
Chromosome Haplogynae meiosis sex chromosome system spider |
description |
Pholcidae is the most diverse family among haplogyne spiders but only 15 species have been analyzed cytogenetically. These studies revealed that the diploid number varies from 2n = 15 to 2n = 32, that there are three types of sex chromosome systems in males (X, X(1)X(2) and X(1)X(2)Y), and that the chromosomes are predominantly biarmed. Within the genus Mesabolivar, only Mesabolivar luteus (Keyserling 1891) has bee karyotyped, and it showed 2n = 15 = 14 + X, with all chromosomes being metacentric. In the present work, we characterize the mitotic and meiotic chromosomes of Mesabolivar brasiliensis (Moenkhaus 1898) and Mesabolivar cyaneotaeniatus (Keyserling 1891). Male mitotic metaphases of the two species showed the diploid number 2n = 17 = 16 + X; oogonial cells of M. brasiliensis showed 2n = 18 = 16 + XX. In bot species, the chromosomes were exclusively biarmed, and the X chromosome was the largest element of the karyotype. Diplotene spermatocytes of the two species exhibited 811 + X and the occurrence of only one terminal or interstitial chiasma per bivalent. In M. cyancotaeniaus, metaphases II with n = 9 = 8 + X and n = 8 were found, indicating the regular segregation of all chromosomes during meiosis I. Mitotic metaphases of M. brasiliensis stained with CMA(3)/DA/DAPI revealed GC-rich chromatin in the terminal region of almost all autosomes, especially in pair 2. An earlier revision of the New World pholeids grouped M. brasiliensis and M. cyaneotaeniatus in a "southern group" and placed M. luteus in a "miscellaneous group." A molecular study showed a closer relationship between M. brasiliensis and M. cyaneotaeniatus than between M. luteus and either of these two species. The 2n = 17 found in M. brasiliensis and M. cyaneotaeniatus corroborates this hypothesis, given that M. luteus has a diploid number of 2n = 15. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T13:55:42Z 2014-05-20T13:55:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1636/CSh07-132.1 Journal of Arachnology. College Park: Amer Arachnological Soc, v. 36, n. 2, p. 453-456, 2008. 0161-8202 http://hdl.handle.net/11449/19947 10.1636/CSh07-132.1 WOS:000260696100028 |
url |
http://dx.doi.org/10.1636/CSh07-132.1 http://hdl.handle.net/11449/19947 |
identifier_str_mv |
Journal of Arachnology. College Park: Amer Arachnological Soc, v. 36, n. 2, p. 453-456, 2008. 0161-8202 10.1636/CSh07-132.1 WOS:000260696100028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Arachnology 1.236 0,557 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
453-456 |
dc.publisher.none.fl_str_mv |
Amer Arachnological Soc |
publisher.none.fl_str_mv |
Amer Arachnological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789828017815552 |