Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fpls.2014.00544 http://hdl.handle.net/11449/117511 |
Resumo: | Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA. |
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Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard linemaizechromosomessatellite DNAcentromereheterochromatic knobsMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, BR-13418900 Piracicaba, SP, BrazilBrazilian Agr Res Corp, Embrapa Cassava & Fruits, Cruz Das Almas, BrazilSao Paulo State Univ, Fac Sci & Letters, Dept Biol Sci, Assis, BrazilUniv Estadual Campinas, Ctr Mol Biol & Genet Engn, Campinas, BrazilHerminio Ometto Fdn, Herminio Ometto Univ Ctr, Araras, BrazilSao Paulo State Univ, Fac Sci & Letters, Dept Biol Sci, Assis, BrazilFAPESP: 98/011289-0Frontiers Research FoundationUniversidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Herminio Ometto FdnMondin, MateusSantos-Serejo, Janay A.Bertão, Mônica Rosa [UNESP]Laborda, PriandaPizzaia, DanielAguiar-Perecin, Margarida L. R.2015-03-18T15:56:20Z2015-03-18T15:56:20Z2014-10-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.3389/fpls.2014.00544Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014.1664-462Xhttp://hdl.handle.net/11449/11751110.3389/fpls.2014.00544WOS:000344766900001WOS000344766900001.pdf4478021043584581Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Plant Science3.678info:eu-repo/semantics/openAccess2024-06-13T17:38:41Zoai:repositorio.unesp.br:11449/117511Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:59:08.585083Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
title |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
spellingShingle |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line Mondin, Mateus maize chromosomes satellite DNA centromere heterochromatic knobs |
title_short |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
title_full |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
title_fullStr |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
title_full_unstemmed |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
title_sort |
Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line |
author |
Mondin, Mateus |
author_facet |
Mondin, Mateus Santos-Serejo, Janay A. Bertão, Mônica Rosa [UNESP] Laborda, Prianda Pizzaia, Daniel Aguiar-Perecin, Margarida L. R. |
author_role |
author |
author2 |
Santos-Serejo, Janay A. Bertão, Mônica Rosa [UNESP] Laborda, Prianda Pizzaia, Daniel Aguiar-Perecin, Margarida L. R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Herminio Ometto Fdn |
dc.contributor.author.fl_str_mv |
Mondin, Mateus Santos-Serejo, Janay A. Bertão, Mônica Rosa [UNESP] Laborda, Prianda Pizzaia, Daniel Aguiar-Perecin, Margarida L. R. |
dc.subject.por.fl_str_mv |
maize chromosomes satellite DNA centromere heterochromatic knobs |
topic |
maize chromosomes satellite DNA centromere heterochromatic knobs |
description |
Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-13 2015-03-18T15:56:20Z 2015-03-18T15:56:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fpls.2014.00544 Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014. 1664-462X http://hdl.handle.net/11449/117511 10.3389/fpls.2014.00544 WOS:000344766900001 WOS000344766900001.pdf 4478021043584581 |
url |
http://dx.doi.org/10.3389/fpls.2014.00544 http://hdl.handle.net/11449/117511 |
identifier_str_mv |
Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014. 1664-462X 10.3389/fpls.2014.00544 WOS:000344766900001 WOS000344766900001.pdf 4478021043584581 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers In Plant Science 3.678 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Research Foundation |
publisher.none.fl_str_mv |
Frontiers Research Foundation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129145749635072 |