Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line

Detalhes bibliográficos
Autor(a) principal: Mondin, Mateus
Data de Publicação: 2014
Outros Autores: Santos-Serejo, Janay A., Bertão, Mônica Rosa [UNESP], Laborda, Prianda, Pizzaia, Daniel, Aguiar-Perecin, Margarida L. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fpls.2014.00544
http://hdl.handle.net/11449/117511
Resumo: Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.
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spelling Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard linemaizechromosomessatellite DNAcentromereheterochromatic knobsMaize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Genet, BR-13418900 Piracicaba, SP, BrazilBrazilian Agr Res Corp, Embrapa Cassava & Fruits, Cruz Das Almas, BrazilSao Paulo State Univ, Fac Sci & Letters, Dept Biol Sci, Assis, BrazilUniv Estadual Campinas, Ctr Mol Biol & Genet Engn, Campinas, BrazilHerminio Ometto Fdn, Herminio Ometto Univ Ctr, Araras, BrazilSao Paulo State Univ, Fac Sci & Letters, Dept Biol Sci, Assis, BrazilFAPESP: 98/011289-0Frontiers Research FoundationUniversidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Herminio Ometto FdnMondin, MateusSantos-Serejo, Janay A.Bertão, Mônica Rosa [UNESP]Laborda, PriandaPizzaia, DanielAguiar-Perecin, Margarida L. R.2015-03-18T15:56:20Z2015-03-18T15:56:20Z2014-10-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://dx.doi.org/10.3389/fpls.2014.00544Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014.1664-462Xhttp://hdl.handle.net/11449/11751110.3389/fpls.2014.00544WOS:000344766900001WOS000344766900001.pdf4478021043584581Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers In Plant Science3.678info:eu-repo/semantics/openAccess2024-06-13T17:38:41Zoai:repositorio.unesp.br:11449/117511Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:59:08.585083Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
title Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
spellingShingle Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
Mondin, Mateus
maize
chromosomes
satellite DNA
centromere
heterochromatic knobs
title_short Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
title_full Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
title_fullStr Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
title_full_unstemmed Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
title_sort Karyotype variability in tropical maize sister inbred lines and hybrids compared with KYS standard line
author Mondin, Mateus
author_facet Mondin, Mateus
Santos-Serejo, Janay A.
Bertão, Mônica Rosa [UNESP]
Laborda, Prianda
Pizzaia, Daniel
Aguiar-Perecin, Margarida L. R.
author_role author
author2 Santos-Serejo, Janay A.
Bertão, Mônica Rosa [UNESP]
Laborda, Prianda
Pizzaia, Daniel
Aguiar-Perecin, Margarida L. R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Herminio Ometto Fdn
dc.contributor.author.fl_str_mv Mondin, Mateus
Santos-Serejo, Janay A.
Bertão, Mônica Rosa [UNESP]
Laborda, Prianda
Pizzaia, Daniel
Aguiar-Perecin, Margarida L. R.
dc.subject.por.fl_str_mv maize
chromosomes
satellite DNA
centromere
heterochromatic knobs
topic maize
chromosomes
satellite DNA
centromere
heterochromatic knobs
description Maize karyotype variability has been extensively investigated. The identification of maize somatic and pachytene chromosomes has improved with the development of fluorescence in situ hybridization (FISH) using tandemly repeated DNA sequences as probes. We identified the somatic chromosomes of sister inbred lines that were derived from a tropical flint maize population (Jac Duro [JD]), and hybrids between them, using FISH probes for the 180-bp knob repeat, centromeric satellite (CentC), centromeric satellite 4 (Cent4), subtelomeric clone 4-12-1, 5S ribosomal DNA and nucleolus organizing region DNA sequences. The observations were integrated with data based on C-banded mitotic metaphases and conventional analysis of pachytene chromosomes. Heterochromatic knobs visible at pachynema were coincident with C-bands and 180-bp FISH signals on somatic chromosomes, and most of them were large. Variation in the presence of some knobs was observed among lines. Small 180-bp knob signals were invariant on the short arms of chromosomes 1, 6, and 9. The subtelomeric 4-12-1 signal was also invariant and useful for identifying some chromosomes. The centromere location of chromosomes 2 and 4 differed from previous reports on standard maize lines. Somatic chromosomes of a JD line and the commonly used KYS line were compared by FISH in a hybrid of these lines. The pairing behavior of chromosomes 2 and 4 at pachytene stage in this hybrid was investigated using FISH with chromosome-specific probes. The homologues were fully synapsed, including the 5S rDNA and CentC sites on chromosome 2, and Cent4 and subtelomeric 4-12-1 sites on chromosome 4. This suggests that homologous chromosomes could pair through differential degrees of chromatin packaging in homologous arms differing in size. The results contribute to current knowledge of maize global diversity and also raise questions concerning the meiotic pairing of homologous chromosomes possibly differing in their amounts of repetitive DNA.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-13
2015-03-18T15:56:20Z
2015-03-18T15:56:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fpls.2014.00544
Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014.
1664-462X
http://hdl.handle.net/11449/117511
10.3389/fpls.2014.00544
WOS:000344766900001
WOS000344766900001.pdf
4478021043584581
url http://dx.doi.org/10.3389/fpls.2014.00544
http://hdl.handle.net/11449/117511
identifier_str_mv Frontiers In Plant Science. Lausanne: Frontiers Research Foundation, v. 5, 12 p., 2014.
1664-462X
10.3389/fpls.2014.00544
WOS:000344766900001
WOS000344766900001.pdf
4478021043584581
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers In Plant Science
3.678
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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