Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/tbed.13545 http://hdl.handle.net/11449/201658 |
Resumo: | The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil. |
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Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder XenarthraanteaterarmadillobartonellaBrazilgenetic diversityslothThe superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPInstituto de Ciências Biomédicas Universidade de São PauloICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e RodoviasFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPFAPESP: 2018/02753-0FAPESP: 2018/13838-6CNPq: 302420/2017-7Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)ICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e RodoviasCalchi, Ana Cláudia [UNESP]Vultão, Juliana GaboardiAlves, Mario HenriqueYogui, Débora ReginaDesbiez, Arnaud Leonard JeanAmaral, Renan Bressianini [UNESP]Santi, Mariele [UNESP]Teixeira, Marta Maria GeraldesWerther, Karin [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2020-12-12T02:38:21Z2020-12-12T02:38:21Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-2033http://dx.doi.org/10.1111/tbed.13545Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020.1865-16821865-1674http://hdl.handle.net/11449/20165810.1111/tbed.135452-s2.0-85082622345Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransboundary and Emerging Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:03:18Zoai:repositorio.unesp.br:11449/201658Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:55.158401Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
title |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
spellingShingle |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra Calchi, Ana Cláudia [UNESP] anteater armadillo bartonella Brazil genetic diversity sloth |
title_short |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
title_full |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
title_fullStr |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
title_full_unstemmed |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
title_sort |
Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra |
author |
Calchi, Ana Cláudia [UNESP] |
author_facet |
Calchi, Ana Cláudia [UNESP] Vultão, Juliana Gaboardi Alves, Mario Henrique Yogui, Débora Regina Desbiez, Arnaud Leonard Jean Amaral, Renan Bressianini [UNESP] Santi, Mariele [UNESP] Teixeira, Marta Maria Geraldes Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author_role |
author |
author2 |
Vultão, Juliana Gaboardi Alves, Mario Henrique Yogui, Débora Regina Desbiez, Arnaud Leonard Jean Amaral, Renan Bressianini [UNESP] Santi, Mariele [UNESP] Teixeira, Marta Maria Geraldes Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) ICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e Rodovias |
dc.contributor.author.fl_str_mv |
Calchi, Ana Cláudia [UNESP] Vultão, Juliana Gaboardi Alves, Mario Henrique Yogui, Débora Regina Desbiez, Arnaud Leonard Jean Amaral, Renan Bressianini [UNESP] Santi, Mariele [UNESP] Teixeira, Marta Maria Geraldes Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] André, Marcos Rogério [UNESP] |
dc.subject.por.fl_str_mv |
anteater armadillo bartonella Brazil genetic diversity sloth |
topic |
anteater armadillo bartonella Brazil genetic diversity sloth |
description |
The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:38:21Z 2020-12-12T02:38:21Z 2020-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/tbed.13545 Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020. 1865-1682 1865-1674 http://hdl.handle.net/11449/201658 10.1111/tbed.13545 2-s2.0-85082622345 |
url |
http://dx.doi.org/10.1111/tbed.13545 http://hdl.handle.net/11449/201658 |
identifier_str_mv |
Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020. 1865-1682 1865-1674 10.1111/tbed.13545 2-s2.0-85082622345 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Transboundary and Emerging Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2020-2033 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129572866097152 |