Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra

Detalhes bibliográficos
Autor(a) principal: Calchi, Ana Cláudia [UNESP]
Data de Publicação: 2020
Outros Autores: Vultão, Juliana Gaboardi, Alves, Mario Henrique, Yogui, Débora Regina, Desbiez, Arnaud Leonard Jean, Amaral, Renan Bressianini [UNESP], Santi, Mariele [UNESP], Teixeira, Marta Maria Geraldes, Werther, Karin [UNESP], Machado, Rosangela Zacarias [UNESP], André, Marcos Rogério [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/tbed.13545
http://hdl.handle.net/11449/201658
Resumo: The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil.
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spelling Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder XenarthraanteaterarmadillobartonellaBrazilgenetic diversityslothThe superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPInstituto de Ciências Biomédicas Universidade de São PauloICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e RodoviasFaculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista UNESPFAPESP: 2018/02753-0FAPESP: 2018/13838-6CNPq: 302420/2017-7Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)ICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e RodoviasCalchi, Ana Cláudia [UNESP]Vultão, Juliana GaboardiAlves, Mario HenriqueYogui, Débora ReginaDesbiez, Arnaud Leonard JeanAmaral, Renan Bressianini [UNESP]Santi, Mariele [UNESP]Teixeira, Marta Maria GeraldesWerther, Karin [UNESP]Machado, Rosangela Zacarias [UNESP]André, Marcos Rogério [UNESP]2020-12-12T02:38:21Z2020-12-12T02:38:21Z2020-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-2033http://dx.doi.org/10.1111/tbed.13545Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020.1865-16821865-1674http://hdl.handle.net/11449/20165810.1111/tbed.135452-s2.0-85082622345Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransboundary and Emerging Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:03:18Zoai:repositorio.unesp.br:11449/201658Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:55.158401Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
title Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
spellingShingle Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
Calchi, Ana Cláudia [UNESP]
anteater
armadillo
bartonella
Brazil
genetic diversity
sloth
title_short Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
title_full Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
title_fullStr Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
title_full_unstemmed Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
title_sort Multi-locus sequencing reveals a novel Bartonella in mammals from the Superorder Xenarthra
author Calchi, Ana Cláudia [UNESP]
author_facet Calchi, Ana Cláudia [UNESP]
Vultão, Juliana Gaboardi
Alves, Mario Henrique
Yogui, Débora Regina
Desbiez, Arnaud Leonard Jean
Amaral, Renan Bressianini [UNESP]
Santi, Mariele [UNESP]
Teixeira, Marta Maria Geraldes
Werther, Karin [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author_role author
author2 Vultão, Juliana Gaboardi
Alves, Mario Henrique
Yogui, Débora Regina
Desbiez, Arnaud Leonard Jean
Amaral, Renan Bressianini [UNESP]
Santi, Mariele [UNESP]
Teixeira, Marta Maria Geraldes
Werther, Karin [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
ICAS – Instituto de Conservação de Animais Silvestres – Projeto Bandeiras e Rodovias
dc.contributor.author.fl_str_mv Calchi, Ana Cláudia [UNESP]
Vultão, Juliana Gaboardi
Alves, Mario Henrique
Yogui, Débora Regina
Desbiez, Arnaud Leonard Jean
Amaral, Renan Bressianini [UNESP]
Santi, Mariele [UNESP]
Teixeira, Marta Maria Geraldes
Werther, Karin [UNESP]
Machado, Rosangela Zacarias [UNESP]
André, Marcos Rogério [UNESP]
dc.subject.por.fl_str_mv anteater
armadillo
bartonella
Brazil
genetic diversity
sloth
topic anteater
armadillo
bartonella
Brazil
genetic diversity
sloth
description The superorder Xenarthra consists of sloths, anteaters and armadillos, mammals originated from South America and currently distributed from the south of North America to the south of South America. The present study aimed to investigate the occurrence and genetic diversity of Bartonella spp. in blood and spleen samples from free-living Xenarthra mammals in the states of São Paulo (SP), Mato Grosso do Sul (MS), Rondônia (RO) and Pará (PA). Based on a quantitative real-time PCR (qPCR) assay, a Bartonella spp. nuoG gene fragment was detected in 1.51% (5/330) of the samples: 4 six-banded armadillos (Euphractus sexcinctus) sampled in the MS and 1 southern tamandua (Tamandua tetradactyla) sampled in the PA. Eight sequences (5 ftsZ, 2 gltA and 1 rpoB) were obtained in the conventional PCR assays. In both phylogenetic analyses based on Bayesian and distance (SplitsTree) methods, the obtained ftsZ, gltA and rpoB sequences were positioned in a distinct clade, but related to B. washoensis. The analysis of SplitsTree and genotype networks based on B. washoensis sequences from several hosts from various localities of the world showed that the sequences of the present study were allocated in a group separated from the other sequences, indicating that they probably originated from median vectors and large numbers of mutational events. Additionally, the analyses performed by BLAST showed low percentages of identities of the sequences obtained in the present study when compared to those previously deposited in GenBank. Therefore, we propose a new Candidatus to Bartonella occurring in Xenarthra in Brazil. The present study was the first to report the occurrence of Bartonella sp. in mammals of the superorder Xenarthra in the world, and it was the first to describe a new Candidatus related to B. washoensis in Brazil.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:38:21Z
2020-12-12T02:38:21Z
2020-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/tbed.13545
Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020.
1865-1682
1865-1674
http://hdl.handle.net/11449/201658
10.1111/tbed.13545
2-s2.0-85082622345
url http://dx.doi.org/10.1111/tbed.13545
http://hdl.handle.net/11449/201658
identifier_str_mv Transboundary and Emerging Diseases, v. 67, n. 5, p. 2020-2033, 2020.
1865-1682
1865-1674
10.1111/tbed.13545
2-s2.0-85082622345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Transboundary and Emerging Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2020-2033
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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