Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jprot.2022.104508 http://hdl.handle.net/11449/234148 |
Resumo: | This study aimed to characterize the proteome of spermatozoa and seminal plasma of 4 purebred dogs (Golden Retriever, Great Dane, Bernese Mountain Dog, and Maremmano-Abruzzese Sheepdog). The ejaculate of 13 dogs was collected, and sperm characteristics were subjectively evaluated. Seminal plasma and sperm cells were separated and prepared individually for mass spectrometry. Data were evaluated by univariate and multivariate statistical analysis. A total of 162 proteins were identified, 47 in spermatozoa, 109 in seminal plasma, and 6 in both samples. Serum albumin in spermatozoa and tubulin alpha-3E chain, acrosin binding protein, and tubulin alpha-3 chain in plasma seminal were statistically relevant. Serum albumin and acrosin binding protein improve the sperm capacitation, acrosome reaction, and seminal quality. The tubulin family proteins are related to structural cell organization and flagella movement, and their presence in seminal plasma may be related to sample handling. According to cluster formation, a high association was observed among Bernese Mountain Dog and Great Dane, Golden Retriever, and Maremmano-Abruzzese Sheepdog for sperm proteins. For seminal plasma proteins, Bernese Mountain Dog, Great Dane, and Maremmano-Abruzzese Sheepdog were related. Further studies on breed-specific proteins in the semen of purebred dogs need to be performed to clarify its fertility roles. Significance: For the first time spermatozoa proteins of dogs are described. The comparison of spermatozoa and seminal plasma proteins of four purebred dogs were performed. These results supporting that differences in semen protein profile of different canine breeds exist, which can improve the biotechnologies of reproduction in this species. |
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Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasmaCanine fertilityMass spectrometryProteinPurebred dogSemenThis study aimed to characterize the proteome of spermatozoa and seminal plasma of 4 purebred dogs (Golden Retriever, Great Dane, Bernese Mountain Dog, and Maremmano-Abruzzese Sheepdog). The ejaculate of 13 dogs was collected, and sperm characteristics were subjectively evaluated. Seminal plasma and sperm cells were separated and prepared individually for mass spectrometry. Data were evaluated by univariate and multivariate statistical analysis. A total of 162 proteins were identified, 47 in spermatozoa, 109 in seminal plasma, and 6 in both samples. Serum albumin in spermatozoa and tubulin alpha-3E chain, acrosin binding protein, and tubulin alpha-3 chain in plasma seminal were statistically relevant. Serum albumin and acrosin binding protein improve the sperm capacitation, acrosome reaction, and seminal quality. The tubulin family proteins are related to structural cell organization and flagella movement, and their presence in seminal plasma may be related to sample handling. According to cluster formation, a high association was observed among Bernese Mountain Dog and Great Dane, Golden Retriever, and Maremmano-Abruzzese Sheepdog for sperm proteins. For seminal plasma proteins, Bernese Mountain Dog, Great Dane, and Maremmano-Abruzzese Sheepdog were related. Further studies on breed-specific proteins in the semen of purebred dogs need to be performed to clarify its fertility roles. Significance: For the first time spermatozoa proteins of dogs are described. The comparison of spermatozoa and seminal plasma proteins of four purebred dogs were performed. These results supporting that differences in semen protein profile of different canine breeds exist, which can improve the biotechnologies of reproduction in this species.São Paulo State University (UNESP) School of Veterinary Medicine and Animal ScienceSão Paulo State University (UNESP) School of Veterinary Medicine and Animal ScienceUniversidade Estadual Paulista (UNESP)Araujo, Michelle Silva [UNESP]de Oliveira Henriques Paulo, Otávio Luís [UNESP]Scott, Caroline [UNESP]Paranzini, Cristiane Sella [UNESP]Codognoto, Viviane Maria [UNESP]de Paula Freitas Dell'Aqua, Camila [UNESP]Papa, Frederico Ozanam [UNESP]de Souza, Fabiana Ferreira [UNESP]2022-05-01T13:41:38Z2022-05-01T13:41:38Z2022-04-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2022.104508Journal of Proteomics, v. 257.1876-77371874-3919http://hdl.handle.net/11449/23414810.1016/j.jprot.2022.1045082-s2.0-85124625969Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2024-09-09T14:06:06Zoai:repositorio.unesp.br:11449/234148Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
title |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
spellingShingle |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma Araujo, Michelle Silva [UNESP] Canine fertility Mass spectrometry Protein Purebred dog Semen |
title_short |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
title_full |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
title_fullStr |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
title_full_unstemmed |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
title_sort |
Insights into the influence of canine breed on proteomics of the spermatozoa and seminal plasma |
author |
Araujo, Michelle Silva [UNESP] |
author_facet |
Araujo, Michelle Silva [UNESP] de Oliveira Henriques Paulo, Otávio Luís [UNESP] Scott, Caroline [UNESP] Paranzini, Cristiane Sella [UNESP] Codognoto, Viviane Maria [UNESP] de Paula Freitas Dell'Aqua, Camila [UNESP] Papa, Frederico Ozanam [UNESP] de Souza, Fabiana Ferreira [UNESP] |
author_role |
author |
author2 |
de Oliveira Henriques Paulo, Otávio Luís [UNESP] Scott, Caroline [UNESP] Paranzini, Cristiane Sella [UNESP] Codognoto, Viviane Maria [UNESP] de Paula Freitas Dell'Aqua, Camila [UNESP] Papa, Frederico Ozanam [UNESP] de Souza, Fabiana Ferreira [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Araujo, Michelle Silva [UNESP] de Oliveira Henriques Paulo, Otávio Luís [UNESP] Scott, Caroline [UNESP] Paranzini, Cristiane Sella [UNESP] Codognoto, Viviane Maria [UNESP] de Paula Freitas Dell'Aqua, Camila [UNESP] Papa, Frederico Ozanam [UNESP] de Souza, Fabiana Ferreira [UNESP] |
dc.subject.por.fl_str_mv |
Canine fertility Mass spectrometry Protein Purebred dog Semen |
topic |
Canine fertility Mass spectrometry Protein Purebred dog Semen |
description |
This study aimed to characterize the proteome of spermatozoa and seminal plasma of 4 purebred dogs (Golden Retriever, Great Dane, Bernese Mountain Dog, and Maremmano-Abruzzese Sheepdog). The ejaculate of 13 dogs was collected, and sperm characteristics were subjectively evaluated. Seminal plasma and sperm cells were separated and prepared individually for mass spectrometry. Data were evaluated by univariate and multivariate statistical analysis. A total of 162 proteins were identified, 47 in spermatozoa, 109 in seminal plasma, and 6 in both samples. Serum albumin in spermatozoa and tubulin alpha-3E chain, acrosin binding protein, and tubulin alpha-3 chain in plasma seminal were statistically relevant. Serum albumin and acrosin binding protein improve the sperm capacitation, acrosome reaction, and seminal quality. The tubulin family proteins are related to structural cell organization and flagella movement, and their presence in seminal plasma may be related to sample handling. According to cluster formation, a high association was observed among Bernese Mountain Dog and Great Dane, Golden Retriever, and Maremmano-Abruzzese Sheepdog for sperm proteins. For seminal plasma proteins, Bernese Mountain Dog, Great Dane, and Maremmano-Abruzzese Sheepdog were related. Further studies on breed-specific proteins in the semen of purebred dogs need to be performed to clarify its fertility roles. Significance: For the first time spermatozoa proteins of dogs are described. The comparison of spermatozoa and seminal plasma proteins of four purebred dogs were performed. These results supporting that differences in semen protein profile of different canine breeds exist, which can improve the biotechnologies of reproduction in this species. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T13:41:38Z 2022-05-01T13:41:38Z 2022-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jprot.2022.104508 Journal of Proteomics, v. 257. 1876-7737 1874-3919 http://hdl.handle.net/11449/234148 10.1016/j.jprot.2022.104508 2-s2.0-85124625969 |
url |
http://dx.doi.org/10.1016/j.jprot.2022.104508 http://hdl.handle.net/11449/234148 |
identifier_str_mv |
Journal of Proteomics, v. 257. 1876-7737 1874-3919 10.1016/j.jprot.2022.104508 2-s2.0-85124625969 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021349177950208 |