Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.actatropica.2017.06.007 http://hdl.handle.net/11449/178975 |
Resumo: | Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi from the genus Paracoccidioides in Latin America. PCM-patients (PCM-p) are classified as having acute/subacute or chronic (CF) clinical forms. CF is responsible for 75%–90% of all cases, affects mainly adults over 30 years old and the clinical manifestation are associated mainly with lungs and mucosa of upper airdigestive tract. In addition, the CF patients exhibit fibrosis of the lungs, oral mucous membranes and adrenals, and pulmonary emphysema. Consequently, CF PCM-p with active disease, as well as those that have been apparently cured, seem to be an interesting model for studies aiming to understand the long-term host-fungi relationship and hypoxia. Dendritic cells (DCs) constitute a system that serve as a major link between innate and adaptive immunity composed of several subpopulations of cells including two main subsets: myeloid (mDCs) and plasmacytoid (pDCs). The present study aimed to access the distribution of PBDC subsets of CF PCM-p who were not treated (NT) or treated (apparently cured – AC). CF PCM-p were categorized into two groups, consisting of 9 NTs and 9 ACs. Twenty-one healthy individuals were used as the control group. The determination of the PBDC subsets was performed by FACS (fluorescence-activated cell sorting) and the dosage of serum TNF-α, IL1β, IL-18, CCL3, IL-10 and basic fibroblast growth factor (bFGF) by ELISA (enzyme-linked immunosorbent assay). A high count and percentage of mDCs was observed before treatment, along with a low count of pDCs in treated patients. Furthermore, the mDC:pDC ratio and serum levels of TNF-α was higher in both of the PCM-p groups than in the control group. In conclusion, our findings demonstrated that active PCM influences the distribution of mDCs and pDCs, and after treatment, PCM-p retained a lower count of pDCs associated with pro-inflammatory profile. Therefore, we identified new evidences of persistent immunological abnormalities in PCM-p after treatment. Even these patients showing fungal clearance after successful antifungal treatment; the hypoxia, triggered by the persistent pulmonary sequelae, possibly continues to interfere in the immune response. |
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Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosisAntifungal treatmentEmphysemaMyeloid dendritic cellsParacoccidioidesPlasmacytoid dendritic cellsPulmonary fibrosisParacoccidioidomycosis (PCM) is a systemic mycosis caused by fungi from the genus Paracoccidioides in Latin America. PCM-patients (PCM-p) are classified as having acute/subacute or chronic (CF) clinical forms. CF is responsible for 75%–90% of all cases, affects mainly adults over 30 years old and the clinical manifestation are associated mainly with lungs and mucosa of upper airdigestive tract. In addition, the CF patients exhibit fibrosis of the lungs, oral mucous membranes and adrenals, and pulmonary emphysema. Consequently, CF PCM-p with active disease, as well as those that have been apparently cured, seem to be an interesting model for studies aiming to understand the long-term host-fungi relationship and hypoxia. Dendritic cells (DCs) constitute a system that serve as a major link between innate and adaptive immunity composed of several subpopulations of cells including two main subsets: myeloid (mDCs) and plasmacytoid (pDCs). The present study aimed to access the distribution of PBDC subsets of CF PCM-p who were not treated (NT) or treated (apparently cured – AC). CF PCM-p were categorized into two groups, consisting of 9 NTs and 9 ACs. Twenty-one healthy individuals were used as the control group. The determination of the PBDC subsets was performed by FACS (fluorescence-activated cell sorting) and the dosage of serum TNF-α, IL1β, IL-18, CCL3, IL-10 and basic fibroblast growth factor (bFGF) by ELISA (enzyme-linked immunosorbent assay). A high count and percentage of mDCs was observed before treatment, along with a low count of pDCs in treated patients. Furthermore, the mDC:pDC ratio and serum levels of TNF-α was higher in both of the PCM-p groups than in the control group. In conclusion, our findings demonstrated that active PCM influences the distribution of mDCs and pDCs, and after treatment, PCM-p retained a lower count of pDCs associated with pro-inflammatory profile. Therefore, we identified new evidences of persistent immunological abnormalities in PCM-p after treatment. Even these patients showing fungal clearance after successful antifungal treatment; the hypoxia, triggered by the persistent pulmonary sequelae, possibly continues to interfere in the immune response.Faculdade de Medicina de Botucatu UNESP − Univ Estadual Paulista, Distrito de Rubião Junior s/nFaculdade de Ciências UNESP − Univ Estadual Paulista, Av. Eng. Luiz Edmundo C. Coube 14-01Universidade do Oeste Paulista − UNOESTE, Rua José Bongiovani, 700Faculdade de Medicina de Botucatu UNESP − Univ Estadual Paulista, Distrito de Rubião Junior s/nFaculdade de Ciências UNESP − Univ Estadual Paulista, Av. Eng. Luiz Edmundo C. Coube 14-01Universidade Estadual Paulista (Unesp)Universidade do Oeste Paulista − UNOESTEVenturini, James [UNESP]Cavalcante, Ricardo Souza [UNESP]Moris, Daniela VanessaGolim, Márjorie de Assis [UNESP]Levorato, Adriele Dandara [UNESP]Reis, Karoline Hagatha dos [UNESP]Arruda, Maria Sueli Parreira de [UNESP]Mendes, Rinaldo Poncio [UNESP]2018-12-11T17:32:58Z2018-12-11T17:32:58Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article185-190application/pdfhttp://dx.doi.org/10.1016/j.actatropica.2017.06.007Acta Tropica, v. 173, p. 185-190.1873-62540001-706Xhttp://hdl.handle.net/11449/17897510.1016/j.actatropica.2017.06.0072-s2.0-850216303122-s2.0-85021630312.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2024-08-15T15:23:00Zoai:repositorio.unesp.br:11449/178975Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
title |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
spellingShingle |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis Venturini, James [UNESP] Antifungal treatment Emphysema Myeloid dendritic cells Paracoccidioides Plasmacytoid dendritic cells Pulmonary fibrosis |
title_short |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
title_full |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
title_fullStr |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
title_full_unstemmed |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
title_sort |
Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis |
author |
Venturini, James [UNESP] |
author_facet |
Venturini, James [UNESP] Cavalcante, Ricardo Souza [UNESP] Moris, Daniela Vanessa Golim, Márjorie de Assis [UNESP] Levorato, Adriele Dandara [UNESP] Reis, Karoline Hagatha dos [UNESP] Arruda, Maria Sueli Parreira de [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author_role |
author |
author2 |
Cavalcante, Ricardo Souza [UNESP] Moris, Daniela Vanessa Golim, Márjorie de Assis [UNESP] Levorato, Adriele Dandara [UNESP] Reis, Karoline Hagatha dos [UNESP] Arruda, Maria Sueli Parreira de [UNESP] Mendes, Rinaldo Poncio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade do Oeste Paulista − UNOESTE |
dc.contributor.author.fl_str_mv |
Venturini, James [UNESP] Cavalcante, Ricardo Souza [UNESP] Moris, Daniela Vanessa Golim, Márjorie de Assis [UNESP] Levorato, Adriele Dandara [UNESP] Reis, Karoline Hagatha dos [UNESP] Arruda, Maria Sueli Parreira de [UNESP] Mendes, Rinaldo Poncio [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal treatment Emphysema Myeloid dendritic cells Paracoccidioides Plasmacytoid dendritic cells Pulmonary fibrosis |
topic |
Antifungal treatment Emphysema Myeloid dendritic cells Paracoccidioides Plasmacytoid dendritic cells Pulmonary fibrosis |
description |
Paracoccidioidomycosis (PCM) is a systemic mycosis caused by fungi from the genus Paracoccidioides in Latin America. PCM-patients (PCM-p) are classified as having acute/subacute or chronic (CF) clinical forms. CF is responsible for 75%–90% of all cases, affects mainly adults over 30 years old and the clinical manifestation are associated mainly with lungs and mucosa of upper airdigestive tract. In addition, the CF patients exhibit fibrosis of the lungs, oral mucous membranes and adrenals, and pulmonary emphysema. Consequently, CF PCM-p with active disease, as well as those that have been apparently cured, seem to be an interesting model for studies aiming to understand the long-term host-fungi relationship and hypoxia. Dendritic cells (DCs) constitute a system that serve as a major link between innate and adaptive immunity composed of several subpopulations of cells including two main subsets: myeloid (mDCs) and plasmacytoid (pDCs). The present study aimed to access the distribution of PBDC subsets of CF PCM-p who were not treated (NT) or treated (apparently cured – AC). CF PCM-p were categorized into two groups, consisting of 9 NTs and 9 ACs. Twenty-one healthy individuals were used as the control group. The determination of the PBDC subsets was performed by FACS (fluorescence-activated cell sorting) and the dosage of serum TNF-α, IL1β, IL-18, CCL3, IL-10 and basic fibroblast growth factor (bFGF) by ELISA (enzyme-linked immunosorbent assay). A high count and percentage of mDCs was observed before treatment, along with a low count of pDCs in treated patients. Furthermore, the mDC:pDC ratio and serum levels of TNF-α was higher in both of the PCM-p groups than in the control group. In conclusion, our findings demonstrated that active PCM influences the distribution of mDCs and pDCs, and after treatment, PCM-p retained a lower count of pDCs associated with pro-inflammatory profile. Therefore, we identified new evidences of persistent immunological abnormalities in PCM-p after treatment. Even these patients showing fungal clearance after successful antifungal treatment; the hypoxia, triggered by the persistent pulmonary sequelae, possibly continues to interfere in the immune response. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-12-11T17:32:58Z 2018-12-11T17:32:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.actatropica.2017.06.007 Acta Tropica, v. 173, p. 185-190. 1873-6254 0001-706X http://hdl.handle.net/11449/178975 10.1016/j.actatropica.2017.06.007 2-s2.0-85021630312 2-s2.0-85021630312.pdf |
url |
http://dx.doi.org/10.1016/j.actatropica.2017.06.007 http://hdl.handle.net/11449/178975 |
identifier_str_mv |
Acta Tropica, v. 173, p. 185-190. 1873-6254 0001-706X 10.1016/j.actatropica.2017.06.007 2-s2.0-85021630312 2-s2.0-85021630312.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Tropica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
185-190 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128157165813760 |