Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1742-4658.2008.06480.x http://hdl.handle.net/11449/70454 |
Resumo: | We have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene contains four exons separated by three introns. The coding region of the gene and expressed sequence tags code for a polypeptide of 131 amino acids (14 kDa, pI 6.59). The putative zebrafish Fabp6 protein shared greatest sequence identity with human FABP6 (55.3%) compared to other orthologous mammalian FABPs and paralogous zebrafish Fabps. Phylogenetic analysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian FABP6s. The zebrafish fabp6 gene was assigned to linkage group (chromosome) 21 by radiation hybrid mapping. Conserved gene synteny was evident between the zebrafish fabp6 gene on chromosome 21 and the FABP6/Fabp6 genes on human chromosome 5, rat chromosome 10 and mouse chromosome 11. Zebrafish fabp6 transcripts were first detected in the distal region of the intestine of embryos at 72 h postfertilization. This spatial distribution remained constant to 7-day-old larvae, the last stage assayed during larval development. In adult zebrafish, fabp6 transcripts were detected by RT-PCR in RNA extracted from liver, heart, intestine, ovary and kidney (most likely adrenal tissue), but not in RNA from skin, brain, gill, eye or muscle. In situ hybridization of a fabp6 riboprobe to adult zebrafish sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intestine, and to a lesser extent in ovary and liver, a transcript distribution that is similar, but not identical, to that seen for the mammalian FABP6/Fabp6 gene. © 2008 The Authors. |
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Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio)Adrenal glandConserved gene syntenyIleumIn situ hybridizationLinkage group assignmentfatty acid binding proteinfatty acid binding protein 6amino acid sequencechromosome 11chromosome 21chromosome 5controlled studydevelopmental stageexonexpressed sequence taggenetic linkagegenetic transcriptionin situ hybridizationnonhumannucleotide sequencephylogenypriority journalradiation hybrid mappingreverse transcription polymerase chain reactiontissue distributionzebra fishAmino Acid SequenceAnimalsBase SequenceDNA, ComplementaryFatty Acid-Binding ProteinsGastrointestinal HormonesGene Expression Regulation, DevelopmentalIn Situ HybridizationMolecular Sequence DataMultigene FamilyPhylogenySequence Homology, Amino AcidTime FactorsTissue DistributionTranscription, GeneticZebrafishDanio rerioMammaliaRattusVertebrataWe have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene contains four exons separated by three introns. The coding region of the gene and expressed sequence tags code for a polypeptide of 131 amino acids (14 kDa, pI 6.59). The putative zebrafish Fabp6 protein shared greatest sequence identity with human FABP6 (55.3%) compared to other orthologous mammalian FABPs and paralogous zebrafish Fabps. Phylogenetic analysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian FABP6s. The zebrafish fabp6 gene was assigned to linkage group (chromosome) 21 by radiation hybrid mapping. Conserved gene synteny was evident between the zebrafish fabp6 gene on chromosome 21 and the FABP6/Fabp6 genes on human chromosome 5, rat chromosome 10 and mouse chromosome 11. Zebrafish fabp6 transcripts were first detected in the distal region of the intestine of embryos at 72 h postfertilization. This spatial distribution remained constant to 7-day-old larvae, the last stage assayed during larval development. In adult zebrafish, fabp6 transcripts were detected by RT-PCR in RNA extracted from liver, heart, intestine, ovary and kidney (most likely adrenal tissue), but not in RNA from skin, brain, gill, eye or muscle. In situ hybridization of a fabp6 riboprobe to adult zebrafish sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intestine, and to a lesser extent in ovary and liver, a transcript distribution that is similar, but not identical, to that seen for the mammalian FABP6/Fabp6 gene. © 2008 The Authors.Department of Biology Dalhousie University, Halifax, NS B3H 4J1Departamento de Genética Instituto de Biociências UNESP - Universidade Estadual Paulista, CEP 18618, Botucatu, São PauloDepartment of Biology Dalhousie University, Halifax, NSDepartment of Pharmacology, Halifax, NSDepartment of Cell Biology University of Virginia Health Sciences Center, Charlottesville, VADepartamento de Genética Instituto de Biociências UNESP - Universidade Estadual Paulista, CEP 18618, Botucatu, São PauloDalhousie UniversityUniversidade Estadual Paulista (Unesp)University of Virginia (UVA)Alves-Costa, Fernanda A. [UNESP]Denovan-Wright, Eileen M.Thisse, ChristineThisse, BernardWright, Jonathan M.2014-05-27T11:23:35Z2014-05-27T11:23:35Z2008-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3325-3334application/pdfhttp://dx.doi.org/10.1111/j.1742-4658.2008.06480.xFEBS Journal, v. 275, n. 13, p. 3325-3334, 2008.1742-464X1742-4658http://hdl.handle.net/11449/7045410.1111/j.1742-4658.2008.06480.x2-s2.0-449491491042-s2.0-44949149104.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFEBS Journal4.530info:eu-repo/semantics/openAccess2023-10-21T06:07:16Zoai:repositorio.unesp.br:11449/70454Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-21T06:07:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
title |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
spellingShingle |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) Alves-Costa, Fernanda A. [UNESP] Adrenal gland Conserved gene synteny Ileum In situ hybridization Linkage group assignment fatty acid binding protein fatty acid binding protein 6 amino acid sequence chromosome 11 chromosome 21 chromosome 5 controlled study developmental stage exon expressed sequence tag genetic linkage genetic transcription in situ hybridization nonhuman nucleotide sequence phylogeny priority journal radiation hybrid mapping reverse transcription polymerase chain reaction tissue distribution zebra fish Amino Acid Sequence Animals Base Sequence DNA, Complementary Fatty Acid-Binding Proteins Gastrointestinal Hormones Gene Expression Regulation, Developmental In Situ Hybridization Molecular Sequence Data Multigene Family Phylogeny Sequence Homology, Amino Acid Time Factors Tissue Distribution Transcription, Genetic Zebrafish Danio rerio Mammalia Rattus Vertebrata |
title_short |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
title_full |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
title_fullStr |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
title_full_unstemmed |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
title_sort |
Spatio-temporal distribution of fatty acid-binding protein 6 (fabp6) gene transcripts in the developing and adult zebrafish (Danio rerio) |
author |
Alves-Costa, Fernanda A. [UNESP] |
author_facet |
Alves-Costa, Fernanda A. [UNESP] Denovan-Wright, Eileen M. Thisse, Christine Thisse, Bernard Wright, Jonathan M. |
author_role |
author |
author2 |
Denovan-Wright, Eileen M. Thisse, Christine Thisse, Bernard Wright, Jonathan M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Dalhousie University Universidade Estadual Paulista (Unesp) University of Virginia (UVA) |
dc.contributor.author.fl_str_mv |
Alves-Costa, Fernanda A. [UNESP] Denovan-Wright, Eileen M. Thisse, Christine Thisse, Bernard Wright, Jonathan M. |
dc.subject.por.fl_str_mv |
Adrenal gland Conserved gene synteny Ileum In situ hybridization Linkage group assignment fatty acid binding protein fatty acid binding protein 6 amino acid sequence chromosome 11 chromosome 21 chromosome 5 controlled study developmental stage exon expressed sequence tag genetic linkage genetic transcription in situ hybridization nonhuman nucleotide sequence phylogeny priority journal radiation hybrid mapping reverse transcription polymerase chain reaction tissue distribution zebra fish Amino Acid Sequence Animals Base Sequence DNA, Complementary Fatty Acid-Binding Proteins Gastrointestinal Hormones Gene Expression Regulation, Developmental In Situ Hybridization Molecular Sequence Data Multigene Family Phylogeny Sequence Homology, Amino Acid Time Factors Tissue Distribution Transcription, Genetic Zebrafish Danio rerio Mammalia Rattus Vertebrata |
topic |
Adrenal gland Conserved gene synteny Ileum In situ hybridization Linkage group assignment fatty acid binding protein fatty acid binding protein 6 amino acid sequence chromosome 11 chromosome 21 chromosome 5 controlled study developmental stage exon expressed sequence tag genetic linkage genetic transcription in situ hybridization nonhuman nucleotide sequence phylogeny priority journal radiation hybrid mapping reverse transcription polymerase chain reaction tissue distribution zebra fish Amino Acid Sequence Animals Base Sequence DNA, Complementary Fatty Acid-Binding Proteins Gastrointestinal Hormones Gene Expression Regulation, Developmental In Situ Hybridization Molecular Sequence Data Multigene Family Phylogeny Sequence Homology, Amino Acid Time Factors Tissue Distribution Transcription, Genetic Zebrafish Danio rerio Mammalia Rattus Vertebrata |
description |
We have determined the structure of the fatty acid-binding protein 6 (fabp6) gene and the tissue-specific distribution of its transcripts in embryos, larvae and adult zebrafish (Danio rerio). Like most members of the vertebrate FABP multigene family, the zebrafish fabp6 gene contains four exons separated by three introns. The coding region of the gene and expressed sequence tags code for a polypeptide of 131 amino acids (14 kDa, pI 6.59). The putative zebrafish Fabp6 protein shared greatest sequence identity with human FABP6 (55.3%) compared to other orthologous mammalian FABPs and paralogous zebrafish Fabps. Phylogenetic analysis showed that the zebrafish Fabp6 formed a distinct clade with the mammalian FABP6s. The zebrafish fabp6 gene was assigned to linkage group (chromosome) 21 by radiation hybrid mapping. Conserved gene synteny was evident between the zebrafish fabp6 gene on chromosome 21 and the FABP6/Fabp6 genes on human chromosome 5, rat chromosome 10 and mouse chromosome 11. Zebrafish fabp6 transcripts were first detected in the distal region of the intestine of embryos at 72 h postfertilization. This spatial distribution remained constant to 7-day-old larvae, the last stage assayed during larval development. In adult zebrafish, fabp6 transcripts were detected by RT-PCR in RNA extracted from liver, heart, intestine, ovary and kidney (most likely adrenal tissue), but not in RNA from skin, brain, gill, eye or muscle. In situ hybridization of a fabp6 riboprobe to adult zebrafish sections revealed intense hybridization signals in the adrenal homolog of the kidney and the distal region of the intestine, and to a lesser extent in ovary and liver, a transcript distribution that is similar, but not identical, to that seen for the mammalian FABP6/Fabp6 gene. © 2008 The Authors. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07-01 2014-05-27T11:23:35Z 2014-05-27T11:23:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1742-4658.2008.06480.x FEBS Journal, v. 275, n. 13, p. 3325-3334, 2008. 1742-464X 1742-4658 http://hdl.handle.net/11449/70454 10.1111/j.1742-4658.2008.06480.x 2-s2.0-44949149104 2-s2.0-44949149104.pdf |
url |
http://dx.doi.org/10.1111/j.1742-4658.2008.06480.x http://hdl.handle.net/11449/70454 |
identifier_str_mv |
FEBS Journal, v. 275, n. 13, p. 3325-3334, 2008. 1742-464X 1742-4658 10.1111/j.1742-4658.2008.06480.x 2-s2.0-44949149104 2-s2.0-44949149104.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEBS Journal 4.530 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3325-3334 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649479688060928 |