Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses

Detalhes bibliográficos
Autor(a) principal: Abrantes, Daniele Carvalho [UNESP]
Data de Publicação: 2022
Outros Autores: Rogerio, Carolina Barbara [UNESP], Campos, Estefânia Vangelie Ramos [UNESP], Germano-Costa, Tais, Vigato, Aryane Alves, Machado, Ian Pompermeyer, Sepulveda, Anderson Ferreira, Lima, Renata, de Araujo, Daniele Ribeiro, Fraceto, Leonardo Fernandes [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12951-022-01729-7
http://hdl.handle.net/11449/249456
Resumo: Dengue, yellow fever, Chinkungunya, Zika virus, and West Nile fever have infected millions and killed a considerable number of humans since their emergence. These arboviruses are transmitted by mosquito bites and topical chemical repellents are the most commonly used method to protect against vector arthropod species. This study aimed to develop a new generation of repellent formulations to promote improved arboviruses transmission control. A repellent system based on polycaprolactone (PCL)-polymeric nanoparticles was developed for the dual encapsulation of IR3535 and geraniol and further incorporation into a thermosensitive hydrogel. The physicochemical and morphological parameters of the prepared formulations were evaluated by dynamic light scattering (DLS), nano tracking analysis (NTA), atomic force microscopy (AFM). In vitro release mechanisms and permeation performance were evaluated before and after nanoparticles incorporation into the hydrogels. FTIR analysis was performed to evaluate the effect of formulation epidermal contact. Potential cytotoxicity was evaluated using the MTT reduction test and disc diffusion methods. The nanoparticle formulations were stable over 120 days with encapsulation efficiency (EE) of 60% and 99% for IR3535 and geraniol, respectively. AFM analysis revealed a spherical nanoparticle morphology. After 24 h, 7 ± 0.1% and 83 ± 2% of the GRL and IR3535, respectively, were released while the same formulation incorporated in poloxamer 407 hydrogel released 11 ± 0.9% and 29 ± 3% of the loaded GRL and IR3535, respectively. GRL permeation from PCL nanoparticles and PCL nanoparticles in the hydrogel showed similar profiles, while IR3535 permeation was modulated by formulation compositions. Differences in IR3535 permeated amounts were higher for PCL nanoparticles in the hydrogels (36.9 ± 1.1 mg/cm2) compared to the IR3535-PCL nanoparticles (29.2 ± 1.5 mg/cm2). However, both active permeation concentrations were low at 24 h, indicating that the formulations (PCL nanoparticles and PCL in hydrogel) controlled the bioactive percutaneous absorption. Minor changes in the stratum corneum (SC) caused by interaction with the formulations may not represent a consumer safety risk. The cytotoxicity results presented herein indicate the carrier systems based on poly-epsilon caprolactone (PCL) exhibited a reduced toxic effect when compared to emulsions, opening perspectives for these systems to be used as a tool to prolong protection times with lower active repellent concentrations.
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spelling Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arbovirusesArbovirusesGeraniolInsect repellentIR3535PCL nanoparticleDengue, yellow fever, Chinkungunya, Zika virus, and West Nile fever have infected millions and killed a considerable number of humans since their emergence. These arboviruses are transmitted by mosquito bites and topical chemical repellents are the most commonly used method to protect against vector arthropod species. This study aimed to develop a new generation of repellent formulations to promote improved arboviruses transmission control. A repellent system based on polycaprolactone (PCL)-polymeric nanoparticles was developed for the dual encapsulation of IR3535 and geraniol and further incorporation into a thermosensitive hydrogel. The physicochemical and morphological parameters of the prepared formulations were evaluated by dynamic light scattering (DLS), nano tracking analysis (NTA), atomic force microscopy (AFM). In vitro release mechanisms and permeation performance were evaluated before and after nanoparticles incorporation into the hydrogels. FTIR analysis was performed to evaluate the effect of formulation epidermal contact. Potential cytotoxicity was evaluated using the MTT reduction test and disc diffusion methods. The nanoparticle formulations were stable over 120 days with encapsulation efficiency (EE) of 60% and 99% for IR3535 and geraniol, respectively. AFM analysis revealed a spherical nanoparticle morphology. After 24 h, 7 ± 0.1% and 83 ± 2% of the GRL and IR3535, respectively, were released while the same formulation incorporated in poloxamer 407 hydrogel released 11 ± 0.9% and 29 ± 3% of the loaded GRL and IR3535, respectively. GRL permeation from PCL nanoparticles and PCL nanoparticles in the hydrogel showed similar profiles, while IR3535 permeation was modulated by formulation compositions. Differences in IR3535 permeated amounts were higher for PCL nanoparticles in the hydrogels (36.9 ± 1.1 mg/cm2) compared to the IR3535-PCL nanoparticles (29.2 ± 1.5 mg/cm2). However, both active permeation concentrations were low at 24 h, indicating that the formulations (PCL nanoparticles and PCL in hydrogel) controlled the bioactive percutaneous absorption. Minor changes in the stratum corneum (SC) caused by interaction with the formulations may not represent a consumer safety risk. The cytotoxicity results presented herein indicate the carrier systems based on poly-epsilon caprolactone (PCL) exhibited a reduced toxic effect when compared to emulsions, opening perspectives for these systems to be used as a tool to prolong protection times with lower active repellent concentrations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) Institute of Science and Technology, Avenida Três de Março 511, Alto da Boa Vista, São PauloLaboratory of Bioactivity Assessment and Toxicology of Nanomaterials University of Sorocaba, São PauloHuman and Natural Sciences Center Federal University of ABC, São PauloSão Paulo State University (UNESP) Institute of Science and Technology, Avenida Três de Março 511, Alto da Boa Vista, São PauloFAPESP: #2018/02404-5FAPESP: 2017/24402-1FAPESP: 2018/14734-0FAPESP: 2019/14773-8CAPES: 88887.620205/2021-00Universidade Estadual Paulista (UNESP)University of SorocabaFederal University of ABCAbrantes, Daniele Carvalho [UNESP]Rogerio, Carolina Barbara [UNESP]Campos, Estefânia Vangelie Ramos [UNESP]Germano-Costa, TaisVigato, Aryane AlvesMachado, Ian PompermeyerSepulveda, Anderson FerreiraLima, Renatade Araujo, Daniele RibeiroFraceto, Leonardo Fernandes [UNESP]2023-07-29T15:41:51Z2023-07-29T15:41:51Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12951-022-01729-7Journal of Nanobiotechnology, v. 20, n. 1, 2022.1477-3155http://hdl.handle.net/11449/24945610.1186/s12951-022-01729-72-s2.0-85143653979Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Nanobiotechnologyinfo:eu-repo/semantics/openAccess2023-07-29T15:41:51Zoai:repositorio.unesp.br:11449/249456Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:48:21.251201Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
title Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
spellingShingle Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
Abrantes, Daniele Carvalho [UNESP]
Arboviruses
Geraniol
Insect repellent
IR3535
PCL nanoparticle
title_short Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
title_full Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
title_fullStr Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
title_full_unstemmed Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
title_sort Repellent active ingredients encapsulated in polymeric nanoparticles: potential alternative formulations to control arboviruses
author Abrantes, Daniele Carvalho [UNESP]
author_facet Abrantes, Daniele Carvalho [UNESP]
Rogerio, Carolina Barbara [UNESP]
Campos, Estefânia Vangelie Ramos [UNESP]
Germano-Costa, Tais
Vigato, Aryane Alves
Machado, Ian Pompermeyer
Sepulveda, Anderson Ferreira
Lima, Renata
de Araujo, Daniele Ribeiro
Fraceto, Leonardo Fernandes [UNESP]
author_role author
author2 Rogerio, Carolina Barbara [UNESP]
Campos, Estefânia Vangelie Ramos [UNESP]
Germano-Costa, Tais
Vigato, Aryane Alves
Machado, Ian Pompermeyer
Sepulveda, Anderson Ferreira
Lima, Renata
de Araujo, Daniele Ribeiro
Fraceto, Leonardo Fernandes [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
University of Sorocaba
Federal University of ABC
dc.contributor.author.fl_str_mv Abrantes, Daniele Carvalho [UNESP]
Rogerio, Carolina Barbara [UNESP]
Campos, Estefânia Vangelie Ramos [UNESP]
Germano-Costa, Tais
Vigato, Aryane Alves
Machado, Ian Pompermeyer
Sepulveda, Anderson Ferreira
Lima, Renata
de Araujo, Daniele Ribeiro
Fraceto, Leonardo Fernandes [UNESP]
dc.subject.por.fl_str_mv Arboviruses
Geraniol
Insect repellent
IR3535
PCL nanoparticle
topic Arboviruses
Geraniol
Insect repellent
IR3535
PCL nanoparticle
description Dengue, yellow fever, Chinkungunya, Zika virus, and West Nile fever have infected millions and killed a considerable number of humans since their emergence. These arboviruses are transmitted by mosquito bites and topical chemical repellents are the most commonly used method to protect against vector arthropod species. This study aimed to develop a new generation of repellent formulations to promote improved arboviruses transmission control. A repellent system based on polycaprolactone (PCL)-polymeric nanoparticles was developed for the dual encapsulation of IR3535 and geraniol and further incorporation into a thermosensitive hydrogel. The physicochemical and morphological parameters of the prepared formulations were evaluated by dynamic light scattering (DLS), nano tracking analysis (NTA), atomic force microscopy (AFM). In vitro release mechanisms and permeation performance were evaluated before and after nanoparticles incorporation into the hydrogels. FTIR analysis was performed to evaluate the effect of formulation epidermal contact. Potential cytotoxicity was evaluated using the MTT reduction test and disc diffusion methods. The nanoparticle formulations were stable over 120 days with encapsulation efficiency (EE) of 60% and 99% for IR3535 and geraniol, respectively. AFM analysis revealed a spherical nanoparticle morphology. After 24 h, 7 ± 0.1% and 83 ± 2% of the GRL and IR3535, respectively, were released while the same formulation incorporated in poloxamer 407 hydrogel released 11 ± 0.9% and 29 ± 3% of the loaded GRL and IR3535, respectively. GRL permeation from PCL nanoparticles and PCL nanoparticles in the hydrogel showed similar profiles, while IR3535 permeation was modulated by formulation compositions. Differences in IR3535 permeated amounts were higher for PCL nanoparticles in the hydrogels (36.9 ± 1.1 mg/cm2) compared to the IR3535-PCL nanoparticles (29.2 ± 1.5 mg/cm2). However, both active permeation concentrations were low at 24 h, indicating that the formulations (PCL nanoparticles and PCL in hydrogel) controlled the bioactive percutaneous absorption. Minor changes in the stratum corneum (SC) caused by interaction with the formulations may not represent a consumer safety risk. The cytotoxicity results presented herein indicate the carrier systems based on poly-epsilon caprolactone (PCL) exhibited a reduced toxic effect when compared to emulsions, opening perspectives for these systems to be used as a tool to prolong protection times with lower active repellent concentrations.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-01
2023-07-29T15:41:51Z
2023-07-29T15:41:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12951-022-01729-7
Journal of Nanobiotechnology, v. 20, n. 1, 2022.
1477-3155
http://hdl.handle.net/11449/249456
10.1186/s12951-022-01729-7
2-s2.0-85143653979
url http://dx.doi.org/10.1186/s12951-022-01729-7
http://hdl.handle.net/11449/249456
identifier_str_mv Journal of Nanobiotechnology, v. 20, n. 1, 2022.
1477-3155
10.1186/s12951-022-01729-7
2-s2.0-85143653979
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Nanobiotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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